Interactions among Interleukin-6, C-Reactive Protein and Interleukin-6 (-174) G/C Polymorphism in the Pathogenesis of Crohn’s Disease and Ulcerative Colitis

Abstract

-Inflammatory bowel diseases are multifactorial disorders the clinical manifestation of which depends on the interaction among immune response, genetic and environmental factors. There is growing evidence that cytokines and gene polymorphisms have an important role in disease pathogenesis in various populations although molecular mechanism of their signaling and interactions is not fully understood yet. The present study aimed at exploring the effects of interleukin-6, C-reactive protein and interleukin-6 rs1800795 polymorphism on the development of Crohn's disease, ulcerative colitis and inflammatory bowel diseases overall and at determining differences between inflammatory bowel disease patients and healthy controls. A total of 132 inflammatory bowel disease patients and 71 healthy blood donors were investigated. In order to assess the clinical relevance of interleukin-6 and C-reactive protein serum concentration and interleukin-6 rs1800795 single nucleotide polymorphism in patients with Crohn's disease and ulcerative colitis, we performed a cross-sectional, case-control study. Quantitative assessment of serum interleukin-6 and C-reactive protein was performed with solid-phase, enzyme-labeled, chemiluminescent sequential immunometric and immunoturbidimetric assay, respectively. A real-time fluorescence resonance energy transfer-based method on a LightCyclerTM PCR 1.2 was used for genotyping of IL-6 rs1800795 polymorphism. Both interleukin-6 and C-reactive protein serum levels were elevated in Crohn's disease and ulcerative colitis patients. Positive correlations were observed between C-reactive protein and interleukin-6 serum concentration and ulcerative colitis activity index as measured by modified Truelove-Witt's severity index scale. C-reactive protein serum level was higher in Crohn's disease patients without intestinal resection than in Crohn's disease patients with prior intestinal resection. In ulcerative colitis patients, interleukin-6 and C-reactive protein serum levels were statistically significantly higher in CC interleukin-6 genotype in comparison to GG+GC genotype.

Vitamin D levels in the assessment of Crohn's disease activity and their relation to nutritional status and inflammation

“…Boris Takač and colleagues also observed significant differences in IL-6 levels between patients with IBD and healthy subjects. 60 Poullis et al [61][62][63] found higher CRP concentrations in patients with CD. We observed high levels of all inflammatory markers in all patients, especially in those with active disease, which is consistent with the aforementioned findings.…”

“…Boris Takač and colleagues also observed significant differences in IL‐6 levels between patients with IBD and healthy subjects 60 . Poullis et al 61–63 found higher CRP concentrations in patients with CD.…”

Association between High Mobility Group Box 1 Protein Gene (Rs41369348) Polymorphism and Immunoglobulin A Vasculitis in Children

“…Previous studies have clearly suggested that common genetic components may underlie different immune-related diseases ( 38 , 39 ). The etiopathogenesis of IgAV is complex and not fully understood, and may involve a number of interactions between various environmental and genetic factors ( 10 , 11 , 40 ).…”

The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation

“…IL-6 is a common pro-inflammatory cytokine. It has been shown that serum levels of this pro-inflammatory cytokine were significantly increased in IBD patients [18]. Further research found a correlation between IL-6 expression and disease activity in patients with Crohn's disease (CD) and UC [19].…”