Interactions Among Expressed MicroRNAs and mRNAs in the Early Stages of Fowl Adenovirus Aerotype 4-Infected Leghorn Male Hepatocellular Cells

Wu, Ning; Yang, Bo; Wen, Bo; Wang, Ting; Guo, Jiaona; Qi, Xuefeng; Wang, Jingyu

doi:10.3389/fmicb.2020.00831

Cited by 10 publications

(8 citation statements)

References 54 publications

(46 reference statements)

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“…A recent study reported that gga-miR-148a-5p was significantly downregulated during avian leukosis virus subgroup J (ALV-J) infection and directly targeted PDPK1 to inhibit the proliferation and cell cycle of ALV-J-infected CEF cells [28]. Similarly, Wu et al demonstrated that the expression of gga-miR-148a-5p was suppressed in fowl adenovirus aerotype 4-infected hepatocellular cells [29]. Apparently in disagreement, our results showed that gga-miR-148a-5p was upregulated by APEC infection.…”

Section: Discussionmentioning

confidence: 99%

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Sun

Cao

Yang

et al. 2022

IJMS

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Colibacillosis is an acute and chronic avian disease caused by avian pathogenic E. coli (APEC). Previous studies have demonstrated that RIP2 plays a significant role in APEC infection. Moreover, increasing evidence indicates that microRNAs (miRNAs) are involved in host–pathogen interactions and the immune response. However, the role of miRNAs in the host against APEC infection remains unclear. Herein, we attempted to reveal new miRNAs potentially involved in the regulation of the immune and inflammatory response against APEC infection, with a particular focus on those possibly correlated with RIP2 expression, via miRNA-seq, RT-qPCR, Western blotting, dual-luciferase reporter assay, and CCK-8. The results showed that a total of 93 and 148 differentially expressed (DE) miRNAs were identified in the knockdown of RIP2 cells following APEC infection (shRIP2+APEC) vs. knockdown of RIP2 cells (shRIP2) and shRIP2 vs. wild-type cells (WT), respectively. Among those identified DE miRNAs, the biological function of gga-miR-455-5p was investigated. It was found that gga-miR-455-5p regulated by RIP2 was involved in the immune and inflammatory response against APEC infection via targeting of IRF2 to modulate the expression of type I interferons. Additionally, RIP2 could directly regulate the production of the type I interferons. Altogether, these findings highlighted the crucial role of miRNAs, especially gga-miR-455-5p, in host defense against APEC infection.

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Section: Discussionmentioning

confidence: 99%

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Sun

Cao

Yang

et al. 2022

IJMS

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“…As a special class of regulatory factors in cells, miRNAs can regulate viral replication through different ways of action. FAdVcirc_013009 and FAdVcirc_011337 target the gga-miR-7475-5p, which negatively correlated with CREBZF and LFNG during FAdV-4 infection, and these genes were all associated with the host cell response to viral infection ( Wu et al, 2020 ). It is possible that viral circRNAs sequester functions from host miRNAs or viral miRNAs in order to regulate host or viral target genes.…”

Section: Discussionmentioning

confidence: 99%

The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection

Liu

Guo²,

Han³

et al. 2022

Front. Microbiol.

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Circular RNAs (circRNAs) are a new class of noncoding RNAs that play vital roles in many biological processes. Virus infection induces modifications in cellular circRNA transcriptomes and expresses viral circRNAs. The outbreaks of Hydropericardium-hepatitis syndrome (HHS) caused by fowl adenovirus serotype 4 (FAdV-4) have resulted in huge economic losses to the poultry industry worldwide. To investigate the expression of circRNAs during FAdV-4 infection, we performed transcriptome analysis of FAdV-4-infected leghorn male hepatoma (LMH) cells. In total, 19,154 cellular circRNAs and 135 differentially expressed (DE) cellular circRNAs were identified. The characteristics of the DE cellular circRNAs were analyzed and most of them were related to multiple biological processes according to GO and KEGG enrichment analysis. The accuracy of 10 cellular circRNAs were verified by semiquantitative RT-PCR and sequencing. The change trend was consistent with the RNA sequencing results. Moreover, 2014 viral circRNAs were identified and 10 circRNAs were verified by the same methods. Our analysis showed that seven circRNAs with the same 3′ terminal and variable 5′ terminal regions were located at pTP protein and DNA pol protein of FAdV-4, which may be generated via alternative splicing events. Moreover, the expression level of viral circRNAs was closely related to the replication efficiency of the virus and partial of the viral circRNAs promoted the replication of FAdV-4. Competing endogenous RNA analysis further showed that the effects of cellular and viral circRNAs on host or viral genes may act via miRNAs. Collectively, our findings first indicate that FAdV-4 infection induced the differential expression of cellular circRNAs and FAdV-4 also expressed viral circRNAs, some of which affected FAdV-4 replication. These findings will provide new clues for further understanding FAdV-4 and provide a basis for investigating host-virus interactions.

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“…Based on this technology, several studies have revealed that changes in cell gene transcription are an important aspect of the interaction between FAdV-4 and its host [ 38 , 40 , 41 ]. It is worth noting that our previous study showed that the binding and entry of FAdV-4 into LMH cells profoundly affect early cellular miRNA transcription profiles and that cellular miRNAs play an important role in FAdV-4 entry [ 39 ]. As another type of noncoding RNA, lncRNAs play an important regulatory role in the battle between virus and host, involving the transcription of viral genes, transcription of host genes, stability of mRNAs, translation of mRNAs and host antiviral response [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…In view of these findings, lncRNAs are an emerging hotspot in host–virus interaction research. Recently, several studies have investigated the roles of transcription products in the interaction between FAdV-4 and its host [ 38 , 39 , 40 , 41 ] by transcriptome analysis. However, the function of cellular lncRNAs during FAdV-4 infection is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Potential Role of Long Noncoding RNAs in Regulating Fowl Adenovirus Serotype 4-Induced Apoptosis in Leghorn Male Hepatocellular Cells

Wen

Wang

et al. 2021

Viruses

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Hepatitis-hydropericardium syndrome (HHS) is caused by fowl adenovirus serotype 4 (FAdV-4) and has resulted in considerable economic losses to the poultry industry globally. FAdV-4 elicits apoptosis in host cells. Long noncoding RNAs (lncRNAs) have emerged as important regulatory RNAs with profound effects on various biological processes, including apoptosis. However, it remains unknown whether lncRNAs participate in FAdV-4-induced apoptosis. In this study, RNA sequencing was applied to determine the transcription of cellular lncRNA in leghorn male hepatocellular (LMH) cells infected with FAdV-4. Cellular RNA transcription analysis demonstrated that FAdV-4 infection elicited 1798 significantly differentially expressed (DE) lncRNAs in infected LMH cells at 24 h post-infection (hpi) compared to mock control infection. In addition, 2873 DE mRNAs were also found. Target prediction and analyses revealed that 775 DE lncRNAs whose 671 target mRNAs were among the DE mRNAs were involved in several signaling pathways, including the AMPK signaling pathway, p53 signaling pathway and insulin signaling pathway. From these 775 DE lncRNAs, we identified 71 DE lncRNAs related to apoptosis based on their target gene functions. Subsequently, lncRNA 54128 was selected from the 71 identified DE lncRNAs, and its role in FAdV-4-induced apoptosis was verified. LncRNA 54128 interference significantly suppressed the rate of apoptosis, which was accompanied by reduced BMP4 transcription levels. To the best of our knowledge, this is the first study to analyze host lncRNA transcription during FAdV-4 infection. Our findings provide a better understanding of host responses to FAdV-4 infection and provide new directions for understanding the potential association between lncRNAs and FAdV-4 pathogenesis.

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Interactions Among Expressed MicroRNAs and mRNAs in the Early Stages of Fowl Adenovirus Aerotype 4-Infected Leghorn Male Hepatocellular Cells

Cited by 10 publications

References 54 publications

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

The Cellular and Viral circRNAs Induced by Fowl Adenovirus Serotype 4 Infection

Transcriptome Analysis Reveals the Potential Role of Long Noncoding RNAs in Regulating Fowl Adenovirus Serotype 4-Induced Apoptosis in Leghorn Male Hepatocellular Cells

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