Interactional similarities and differences in the protein complex of PCNA and DNA replication factor C between rice and Arabidopsis

Abstract

Background Proliferating cell nuclear antigen (PCNA), a conserved trimeric ring complex, is loaded onto replication fork through a hetero-pentameric AAA+ ATPase complex termed replication factor C (RFC) to maintain genome stability. Although architectures of PCNA-RFC complex in yeast have been revealed, the functions of PCNA and protein-protein interactions of PCNA-RFC complex in higher plants are not very clear. Here, essential regions mediating interactions between PCNA and RFC subunits in Arabidopsis and rice were investigated via yeast-two-hybrid method and bimolecular fluorescence complementation techniques. Results We observed that OsPCNA could interact with all OsRFC subunits, while protein-protein interactions only exist between Arabidopsis RFC2/3/4/5 and AtPCNA1/2. The truncated analyses indicated that the C-terminal of Arabidopsis RFC2/3/4/5 and rice RFC1/2 is essential for binding PCNA while the region of rice RFC3/4/5 mediating interaction with PCNA distributed both at the N- and C-terminal. On the other hand, we found that the C- and N-terminal of Arabidopsis and rice PCNA contribute equally to PCNA-PCNA interaction, and the interdomain connecting loop (IDCL) domain and C-terminal of PCNAs are indispensable for interacting RFC subunits. Conclusions These results indicated that Arabidopsis and rice PCNAs are highly conserved in sequence, structure and pattern of interacting with other PCNA monomer. Nevertheless, there are also significant differences between the Arabidopsis and rice RFC subunits in binding PCNA. Taken together, our results could be helpful for revealing the biological functions of plant RFC-PCNA complex. Electronic supplementary material The online version of this article (10.1186/s12870-019-1874-z) contains supplementary material, which is available to authorized users.

TaPCNA plays a role in programmed cell death after UV-B exposure in wheat (

“…This result reflects that plant metabolism slows down after PCNA silencing, and growth may be blocked or stopped. These results suggest that after PCNA gene silencing, DNA replication is blocked and affects chromatin synthesis, which leads to cycle block and several instances of PCD (Qian et al 2019). The DNA laddering results showed the diffused DNA of plants after PCNA gene silencing (Fig.…”

Chromatin-remodeling factor CHR721 with non-canonical PIP-box interacts with OsPCNA in Rice

“…Previous work indicated OsPCNA and CHR721 showed similar functions in genomic stability and cell cycle regulation [15,25]. However, whether OsPCNA functions in the reproductive development just as CHR721 is not clear so far because no mutant of OsPCNA has been reported.…”

“…It has been reported that the homologous OsPCNA in rice played a role in the form of homotrimers [9]. OsPCNA could interact with several proteins, such as OsFEN1, OsGEN-L, and all OsRFC subunits [9,15,23].…”

The Armadillo BTB Protein ABAP1 Is a Crucial Player in DNA Replication and Transcription of Nematode-Induced Galls

“…No variation in the PCNA1 mRNA levels in galls suggests that its expression is independent of ABAP1 levels and that minimum concentration might be enough to hold polymerase activity. Also, in plants, PCNA1 and its paralogous PCNA2 are highly conserved genes, with similar sequences, structures, and pattern of protein-protein interaction (Qian et al, 2019), suggesting a redundant function that might overcome some imbalances in their expression as for in galls. PCNA1 is an auxiliary protein of DNA polymerase involved in the control of eukaryotic DNA replication and is the major coordinator of DNA repair at replication forks (Kosugi and Ohashi, 2002;Stevens et al, 2002).…”