Interaction with single-stranded DNA-binding protein modulates Escherichia coli RadD DNA repair activities

Garcia, Miguel A. Osorio; Wood, Elizabeth A.; Keck, James L.; Cox, Michael M.

doi:10.1016/j.jbc.2023.104773

Cited by 5 publications

(2 citation statements)

References 62 publications

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“…The helicase domain of IEE is similar to that of E. coli RadD (Figure S4). RadD binds to fork-structured DNA (Romero et al, 2020) and exhibits ATPase activity facilitated by SSB (Garcia et al, 2023). A mutation in motif I abolishes the ATPase activity of RadD and decreases its DNA-binding capacity in the presence of ATP (Chen et al, 2016).…”

Section: Strand Displacement Activity Of Iee and Its Helicase Domainmentioning

confidence: 99%

“…Each motif is drawn based on Chaar et al (2017) and Chen et al (2015). RecA‐like domains 1 (RD1) and RD2 are highlighted light blue and light green, respectively, which was inferred from data of RadD (Garcia et al, 2023). Dots at the bottom of the aligned sequences show residues acting either as incoming nucleotide ligands (black) or a water molecule ligand (red).…”

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confidence: 94%

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Insertion sequence excision is enhanced by a protein that catalyzes branch migration and promotes microhomology‐mediated end joining

Kishino,

Saito,

Muto

et al. 2023

Genes to Cells

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Insertion sequence (IS)‐excision enhancer (IEE) promotes the excision of ISs in the genome of enterohemorrhagic Escherichia coli O157. Because IEE‐dependent IS excision occurs in the presence of transposase, the process of IS transposition may be involved in IS excision; however, little is understood about the molecular mechanisms of IS excision. Our in vitro analysis revealed that IEE exhibits DNA‐dependent ATPase activity, which is activated by branched DNA. IEE also catalyzes the branch migration of fork‐structured DNA. These results suggest that IEE remodels branched structures of the IS transposition intermediate. Sequence analysis of recombination sites in IS‐excision products suggested that microhomologous sequences near the ends of the IS are involved in IS excision. IEE promoted microhomology‐mediated end joining (MMEJ), in which base pairing between 6‐nucleotides complementary ends of two 3′‐protruding DNAs and subsequent elongation of the paired DNA strand occurred. IS‐excision frequencies were significantly decreased in cells producing IEE mutants that had lost either branch migration or MMEJ activity, which suggests that these activities of IEE are required for IS excision. Based on our results, we propose a model for IS excision triggered by IEE and transposase.

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Section: Strand Displacement Activity Of Iee and Its Helicase Domainmentioning

confidence: 99%

mentioning

confidence: 94%

Insertion sequence excision is enhanced by a protein that catalyzes branch migration and promotes microhomology‐mediated end joining

Kishino,

Saito,

Muto

et al. 2023

Genes to Cells

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show abstract

Structural Basis for the Interaction of Redβ Single-Strand Annealing Protein with Escherichia coli Single-Stranded DNA-Binding Protein

Zakharova,

Liu,

Greenwald

et al. 2024

Journal of Molecular Biology

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Molecular insights into the prototypical single-stranded DNA-binding protein from E. coli

Bonde,

Kozlov,

Cox

et al. 2024

Critical Reviews in Biochemistry and Molecular Biology

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Interaction with single-stranded DNA-binding protein modulates Escherichia coli RadD DNA repair activities

Cited by 5 publications

References 62 publications

Insertion sequence excision is enhanced by a protein that catalyzes branch migration and promotes microhomology‐mediated end joining

Insertion sequence excision is enhanced by a protein that catalyzes branch migration and promotes microhomology‐mediated end joining

Structural Basis for the Interaction of Redβ Single-Strand Annealing Protein with Escherichia coli Single-Stranded DNA-Binding Protein

Molecular insights into the prototypical single-stranded DNA-binding protein from E. coli

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