Interaction with fibronectin regulates cytokine gene expression in human melanoma cells

Lupetti, Raffaella; Mortarini, Roberta; Panceri, Pierangelo; Sensi, Marialuisa; Anichini, Andrea

doi:10.1002/(sici)1097-0215(19960328)66:1<110::aid-ijc19>3.3.co;2-5

Cited by 7 publications

(8 citation statements)

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“…Potential mediators of SPARC signaling are cell surface integrins. In that respect, integrin activation by fibronectin induced IL-8 and GRO expressions in melanoma cells and monocytes (32,33). In addition, integrins modulate leukotriene production and secretion by neutrophils (34).…”

Section: Resultsmentioning

confidence: 99%

Secreted Protein Acidic and Rich in Cysteine Produced by Human Melanoma Cells Modulates Polymorphonuclear Leukocyte Recruitment and Antitumor Cytotoxic Capacity

et al. 2005

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The expression of secreted protein acidic and rich in cysteine (SPARC) has been associated with the malignant progression of different types of human cancer. SPARC was associated with tumor cell capacity to migrate and invade, although its precise role in tumor progression is still elusive. In the present study, we show that SPARC produced by melanoma cells modulates the antitumor activity of polymorphonuclear leukocytes (PMN). Administration to nude mice of human melanoma cells in which SPARC expression was transiently or stably knocked down by antisense RNA (SPARC-sup cells) promoted PMN recruitment and obliterated tumor growth even when SPARC-sup cells accounted for only 10% of injected malignant cells. In addition, SPARC-sup cells stimulated the in vitro migration and triggered the antimelanoma cytotoxic capacity of human PMN, an effect that was reverted in the presence of SPARC purified from melanoma cells or by reexpressing SPARC in SPARC-sup cells. Leukotrienes, interleukin 8, and growth-related oncogene, in combination with Fas ligand and interleukin 1, mediated SPARC effects. These data indicate that SPARC plays an essential role in tumor evasion from immune surveillance through the inhibition of the antitumor PMN activity. (Cancer Res 2005; 65(12): 5123-32)

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Section: Resultsmentioning

confidence: 99%

Secreted Protein Acidic and Rich in Cysteine Produced by Human Melanoma Cells Modulates Polymorphonuclear Leukocyte Recruitment and Antitumor Cytotoxic Capacity

et al. 2005

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“…First‐strand cDNA was synthesized by reverse transcription of 5 μg of total RNA using 200 units of Moloney Murine Leukemia Virus Reverse Transcriptase (M‐MLV RT, Promega Co., Maison, WI, USA) and 500 ng of oligo‐dT primer at 42°C for 1 hour. For amplification of cDNAs, oligonucleotide primers for PCR were designed as shown in Table 2 (10–13). β‐actin was employed as a positive internal control for the suitability of the respective specimen for PCR amplification.…”

Section: Methodsmentioning

confidence: 99%

Expression of Basic Fibroblast Growth Factor, Vascular Endothelial Growth Factor, and Thrombospondin‐1 Related to Microvessel Density in Nonaggressive and Aggressive Basal Cell Carcinomas

Kwon

Kim

et al. 2003

The Journal of Dermatology

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The aggressive forms of basal cell carcinoma (BCC) are biologically different in a number of features from their nonaggressive counterparts. The expression of basic Fibroblast Growth Factor (bFGF), Vascular Endothelial Growth Factor (VEGF), and Thrombospondin-1 (TSP-1) was examined in primary culture samples of nonaggressive and aggressive BCCs. We studied the relationship between neoangiogenesis by counting microvessels, tumor aggressiveness, and the expression of three different angiogenic factors. bFGF mRNA was detectable in all BCC samples, but there was no significant difference in the levels of expression between the two types. VEGF mRNA was also detectable in all BCC samples. VEGF expression in the aggressive type was approximately 3.5-fold higher than in the nonaggressive type. TSP-1 expression was variable and not related to the type of BCC. The mean value of microvessel density (MVD) was significantly higher for the aggressive type than for the nonaggressive type. There was a significant correlation between VEGF levels and MVD. No significant relationships were found between bFGF, TSP-1 mRNA expression, and MVD. In conclusion, the results of present study suggest that VEGF expression and angiogenesis might play important roles in the progression to aggressive BCC.

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“…Human melanoma cell lines used were as follows: (i Primary and metastatic melanoma cell lines were derived from histologically diagnosed primary (cutaneous) and secondary (subcutaneous, lymphonodular, or visceral) melanoma lesions, respectively, as previously described (Lupetti et al, 1996;Jonjic et al, 1992;Mortarini et al, 1991Mortarini et al, , 1992Pietra et al, 2001;Rodolfo et al, 1988). All melanoma cell lines expressed melanoma antigens such as MART-1, gp100, and tyrosinase and members of the MAGE family.…”

Section: Cellsmentioning

confidence: 99%

Potent Phagocytic Activity Discriminates Metastatic and Primary Human Malignant Melanomas: A Key Role of Ezrin

Lugini

Lozupone

Matarrese

et al. 2003

Laboratory Investigation

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SUMMARY:Features of phagocytosis have been observed in human tumors, but the phagocytic apparatus of tumor cells and the mechanism(s) underlying this phenomenon have yet to be defined. To address the phenomenon of phagocytosis, its underlying mechanism(s), and its possible role in tumor biology, we used human melanoma cells as a prototypic model. Our results showed that a process of phagocytosis of apoptotic cells occurs in vivo in human melanoma. This finding was consistent with evidence that human melanoma cells in vitro express all of the known lysosomal and phagocytic markers on their cytoplasmic vesicles and that a process of phagocytosis occurs in these vesicles. However, exclusively human melanoma cells deriving from metastatic lesions possess an efficient phagocytic machinery responsible for a macrophage-like activity against latex beads, yeast, and apoptotic cells of different origins, which was comparable to that of human primary macrophages. Moreover, the actin-binding protein ezrin was expressed on phagocytic vacuoles of melanoma cells and of cells deriving from a human adenocarcinoma; both treatment with cytochalasin B and specific inhibition of ezrin synthesis strongly affected the phagocytic activity of melanoma cells. This suggests that the association with the actin cytoskeleton is a crucial requirement for the development of this phenomenon. Hence our data provide evidence for a potent phagocytic activity exerted by metastatic melanoma cells possibly involved in determining the level of aggressiveness of human melanoma. This suggests that the assessment of phagocytic activity may be exploited as a new tool to evaluate the malignancy of human melanoma. Moreover, our data suggest that gene therapy or drug treatments aimed at inhibiting actin assembly to the phagosomal membranes may be proposed as a new strategy for the control of tumor aggressiveness. (Lab Invest 2003, 83:1555-1567.

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Interaction with fibronectin regulates cytokine gene expression in human melanoma cells

Cited by 7 publications

References 0 publications

Secreted Protein Acidic and Rich in Cysteine Produced by Human Melanoma Cells Modulates Polymorphonuclear Leukocyte Recruitment and Antitumor Cytotoxic Capacity

Secreted Protein Acidic and Rich in Cysteine Produced by Human Melanoma Cells Modulates Polymorphonuclear Leukocyte Recruitment and Antitumor Cytotoxic Capacity

Expression of Basic Fibroblast Growth Factor, Vascular Endothelial Growth Factor, and Thrombospondin‐1 Related to Microvessel Density in Nonaggressive and Aggressive Basal Cell Carcinomas

Potent Phagocytic Activity Discriminates Metastatic and Primary Human Malignant Melanomas: A Key Role of Ezrin

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