Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy

Liu, Yuying; Wang, Peng‐Ye; Dou, S. X.; Lv, Hongfeng

doi:10.1063/1674-0068/27/01/115-120

Cited by 1 publication

(1 citation statement)

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“…Atomic force microscopy (AFM) has been widely used to visualize both local and global DNA topography and intermolecular interaction of DNA molecules . Importantly, transition of short oligonucleotides, interaction of DNA nucleotides with gold surface, characteristics of the interaction of DNA with proteins/small molecules, anticancer drug oxaliplatin, histone proteins, and even nanoparticles can be investigated by AFM‐based force spectroscopy. DNA chains were commonly immobilized on a solid substrate via either divalent or multivalent ions .…”

Section: Introductionmentioning

confidence: 99%

DNA aggregation induced by Mg²⁺ ions under different conditions

Bui

Nguyen

2018

J of Molecular Recognition

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Cations-induced DNA aggregation can modify the local structure of oligonucleotides and has potential applications in medicine and biotechnology. Here, we used atomic force microscopy to investigate λ-DNA aggregation on Mg -treated glass (Mg /glass) and in Mg solution. Atomic force microscopy topography images showed that some DNA fragments were slightly stacked together on 10 mM Mg /glass and stacked stronger on ≥50 mM Mg /glass. They also showed that DNA aggregated stronger in Mg solution than on Mg /glass, ie, DNAs are strongly stacked and twisted at 10 mM Mg , rolled together at 50 mM Mg , and slightly aggregated to form small particles at 100 mM Mg . At a specific condition, ie, heating λ-DNA to 92°C, cooling down to 75°C, adding Mg , and vortexing the resulting solution, DNA strongly aggregated and formed pancake-like shapes at 10 and 50 mM or a large aggregate at 100 mM Mg solutions. Our results may be helpful for medical applications and gene therapy using cation-DNA technology.

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