Interaction patterns between dentin and adhesive on prepared Class V cavitiesin vitro andin vivo

Schneider, Hartmut; Fröhlich, Matthias; Erler, Gitte; Engelke, C.; Merte, K

doi:10.1002/(sici)1097-4636(2000)53:1<86::aid-jbm12>3.0.co;2-z

Cited by 24 publications

(27 citation statements)

References 19 publications

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“…Finally, they were dehydrated in ethanol and hexamethyldisilazane. 17,18 The dentin-resin interdiffusion zones of the 16 hemidisks from each group were observed under fieldemission scanning electron microscope (FESEM) (JEOL JSM 6301F, Tokyo, Japan) at 10 kV, using secondary electrons. Electronic microphotographs were then taken at different magnifications and scanned with the energydispersive spectroscopy microanalysis system (Oxford Inca Energy 350 ® -Oxford Instruments, Oxfordshire, United Kingdom).…”

Section: Methodsmentioning

confidence: 99%

Morphology of the Dentin–resin Interface yielded by Two-step Etch-and-rinse Adhesives with Different Solvents

Ferreira

Pires

Azevedo

et al. 2017

The Journal of Contemporary Dental Practice

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Aim:The study aimed to analyze the morphology of the dentinresin interface yielded by two-step etch-and-rinse adhesive systems with different solvents and compositions. Materials and methods:A total of 32 dentine disks were prepared and randomly assigned to four groups of one-bottle etchand-rinse adhesive systems containing different solvents: group I, Adper Scotchbond-1XT™ (ethanol/water); group II, XP-Bond™ (tertiary butanol); group III, Prime and Bond NT ® (acetone); and group IV, One Coat bond ® (5% water). Adhesive systems were applied onto dentin disks, which were then thermal cycled, divided into two hemi-disks (n = 16), and prepared for field-emission scanning electron microscopy to examine the dentin-resin interdiffusion zone. Microphotographs were scanned and data were processed. Data were compared with analysis of variance multivariant test after Kolmogorov-Smirnov and Shapiro-Wilk tests using Statistic Package for the Social Sciences. Results:The adhesive layer thickness average found was group I: 45.9 ± 13.41 µm, group II: 20.6 ± 16.32 µm, group III: 17.7 ± 11.75 µm, and group IV: 50.7 ± 27.81 µm. Significant differences were found between groups I and IV and groups II and III (p < 0.000).Groups I (3.23 ± 0.53 µm) and II (3.13 ± 0.73 µm) yielded significantly thicker hybrid layers than groups III (2.53 ± 0.50 µm) and IV (1.84 ± 0.27 µm) (p < 0.003). Group III presented a less homogeneous hybrid layer, with some gaps. Tag length average was greater in groups II (111.0 ± 36.92 µm) and IV (128.9 ± 78.38 µm) than in groups I (61.5 ± 18.10 µm) and III (68.6 ± 15.84 µm) (p < 0.008). Conclusion:Adhesives systems with different solvents led to significant differences in the dentin-resin interface morphology. Solvents role in adhesives bond strength should be considered together with the other adhesive system components. Morphology of the Clinical significance:The adhesive containing tertiary butanol, in addition, seems to originate a good-quality hybrid layer and long, entangled tags and also appears to have greater ability to originate microtags, which may indicate higher bond strength.

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Section: Methodsmentioning

confidence: 99%

Morphology of the Dentin–resin Interface yielded by Two-step Etch-and-rinse Adhesives with Different Solvents

Ferreira

Pires

Azevedo

et al. 2017

The Journal of Contemporary Dental Practice

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“…Such a cleft generally demonstrates the site of the weakest compound on a distinct spot within the specimen. 5,61 If the interface withstands all loadings, either a fully preserved specimen or cohesive defects within the section can be observed.…”

Section: Figure 3: Sem Images Of Interfaces (A) On Dentin With Resin mentioning

confidence: 99%

“…Although there is no accepted experimental mode for the early experimental assessment of restorative systems, and clinical trials still remain the ultimate test, [3][4] experimental in vitro/in vivo bond testing may predict the results of clinical studies to a certain degree. 1,[4][5] Between bond strength measurements on enamel and dentin [6][7][8] and studies related to the biocompatibility of system components [9][10][11] and spectroscopic investigations, [12][13] numerous imaging methods can help to characterize restoration systems in great detail by investigating phenomena, such as marginal integrity, microleakage and the mechanisms of mechanical adhesion (interlocking/hybridization of enamel and dentin). 14 Such experimental screenings include investigating tooth substrates of different qualities (sound/carious dentin) 8,[15][16][17][18][19] and C-factors, [20][21][22] simulation of pulpal pressure and the oral environment, [23][24][25] the application of thermal and occlusal stresses, fatigue simulation [26][27] or, for better simulation of the clinical situation, studies on in vivo restored teeth that have been in use for a certain period of time.…”

Section: Introductionmentioning

confidence: 99%

“…14 Such experimental screenings include investigating tooth substrates of different qualities (sound/carious dentin) 8,[15][16][17][18][19] and C-factors, [20][21][22] simulation of pulpal pressure and the oral environment, [23][24][25] the application of thermal and occlusal stresses, fatigue simulation [26][27] or, for better simulation of the clinical situation, studies on in vivo restored teeth that have been in use for a certain period of time. 5,9,25,[28][29][30][31][32][33] In the past, one of the intentions of the authors of the current study was to combine the clinical assessment of enamel-dentin bonding systems with experimental in vivo and in vitro studies on the basis of the micromorphological evaluation of the tooth-composite interface and microleakage formation. 2,5,24,29,[34][35][36][37][38] This kind of experimental proof is, however, aimed at the comparative assessment of restoration systems that are investigated using the same laboratory standard, analogous to the rating of adhesive systems on the basis of bond strength values achieved within a single standardized trial.…”

Section: Introductionmentioning

confidence: 99%

“…5,9,25,[28][29][30][31][32][33] In the past, one of the intentions of the authors of the current study was to combine the clinical assessment of enamel-dentin bonding systems with experimental in vivo and in vitro studies on the basis of the micromorphological evaluation of the tooth-composite interface and microleakage formation. 2,5,24,29,[34][35][36][37][38] This kind of experimental proof is, however, aimed at the comparative assessment of restoration systems that are investigated using the same laboratory standard, analogous to the rating of adhesive systems on the basis of bond strength values achieved within a single standardized trial.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Operator-specific Handling on Tooth-composite Interface and Microleakage Formation

Schneider

Busch²,

Busch³

et al. 2009

Operative Dentistry

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SUMMARYThe aim of this in vitro study was a comparative investigation of tooth-composite interface morphology and microleakage formation after the calibrated restoration of Class II cavities with a proven multi-component, three-step, total-etch adhesive system by three experienced operators. The current study also aimed to acquire data about the well-tried adhesive system under defined laboratory conditions for further comparative assessments with other adhesives. H Schneider • I Busch • M Busch H Jentsch • M Häfer Clinical RelevanceAfter calibration of the restoration procedure, three operators experienced differences in toothcomposite interface morphology and microleakage formation. These differences reflect technique-sensitivity of the etch-and-rinse adhesive. The lowest microleakage on dentin was related to an enhanced interface integrity. After additional application of an adhesive component, sealing of the enamel and dentin was partially enhanced, but microleakage formation did not decrease. These results are helpful in the comparative evaluation of other bonding systems. Schneider & Others: Effect of Operator-specific Handling on Tooth-composite Interface & Microleakage FormationForty Class II cavities were prepared on extracted, caries-free molars. In the first study (Part A), 24 cavities were restored (n=8 each) according to the manufacturers' instructions.In the second study (Part B), 16 teeth (n=6, 4, 6) were filled in the same way; however, the last component of the same adhesive system was applied twice. The teeth were prepared for microleakage testing and morphological assessment of the composite-tooth interface by scanning electron microscopy. The three operators had differences with regard to adhesive layer formation, inter-/peritubular adhesive penetration and the formation of adhesive failures on the dentin interface and microleakage on dentin. The lowest microleakage on dentin was related to enhanced peritubular and intertubular adhesive penetration.After double application of an adhesive component (Part B), the sealing of the enamel and dentin with adhesive increased partially, and adhesive failures on dentin were partly diminished, but no distinct effects on microleakage formation were found.The results confirm the technique-sensitivity of the etch-and-rinse adhesive related to interface morphology and microleakage formation. These results are helpful in the comparative evaluation of other bonding systems.

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The effect of NaOCl dentin treatment on nanoleakage formation

Pioch

Kobašlija

Huseinbegović

et al. 2001

J. Biomed. Mater. Res.

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The term "nanoleakage" has been introduced to explain a penetration pathway within hybrid layers of the dentin-composite junction in the absence of gap formation. This phenomenon is argued in the literature to be a risk factor for the quality of the dentin bonding. NaOCl is a well-known agent used to remove collagen layers that are exposed after acid etching. The purpose of this study was to determine the influence of an NaOCl treatment of dentin to the formation of nanoleakage. Class V cavities were prepared in 40 human molars with the cervical margins located in dentin. After etching with phosphoric acid, 20 samples were treated with 10% NaOCl for 60 s, and 20 samples were used as control. Composite restorations were placed using two different bonding systems. The specimen were exposed in silver nitrate solution and then in developer. After embedding, the teeth were sectioned, dried, coated with gold, and analyzed by SEM. For all the specimen in the control group, backscatter images indicated a silver penetration within the hybrid layer. There was no penetration of silver detectable in the teeth that were treated with NaOCl. We conclude that the removal of the collagen layer by using NaOCl avoids the formation of nanoleakages with the materials tested.

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Interaction patterns between dentin and adhesive on prepared Class V cavitiesin vitro andin vivo

Cited by 24 publications

References 19 publications

Morphology of the Dentin–resin Interface yielded by Two-step Etch-and-rinse Adhesives with Different Solvents

Morphology of the Dentin–resin Interface yielded by Two-step Etch-and-rinse Adhesives with Different Solvents

Effect of Operator-specific Handling on Tooth-composite Interface and Microleakage Formation

The effect of NaOCl dentin treatment on nanoleakage formation

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