Interaction Pathways between Plasma Membrane and Block Copolymer Micelles

Guan, Zhou; Wang, Liquan; Lin, Jiaping

doi:10.1021/acs.biomac.6b01674

Cited by 30 publications

(34 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the complexity of cell membranes is not precisely represented, this coarse‐gained model successfully maintains the essential features of the cell membrane, such as the bending module, fluidity and the binding between ligands and receptors . Using this model, results that agree with experimental observations were gained in existing theoretical simulation work . We believe that this simulation method is capable of revealing the cellular uptake behaviours of nanoparticles.…”

Section: Simulation Methodsmentioning

confidence: 84%

“…When anisotropically shaped nanoparticles are internalized by membranes, the initial contact angles between nanoparticles and membranes can have an effect on the endocytosis behaviours . In the present study, we performed simulations of nanoparticles that have different initial contact angles with the membrane and studied the results of endocytosis.…”

Section: Resultsmentioning

confidence: 99%

“…The molecular potentials and parameters were all set according to those used in published work . For lipids, a finite extensible nonlinear elastic potential was adopted for defining the bonds linking two neighbouring beads:

U_{bond} (r) = - \frac{1}{2} k_{normalb} r_{normalb}^{2} \ln [1 - {((), \frac{r}{r_{b}})}^{2}]

…”

Section: Simulation Methodsmentioning

confidence: 99%

“…The value of r a is set to be larger than 2 r b (3 σ ) so that the stiffness of lipids in the simulations can be maintained. This value was also adopted in some reported simulation work …”

Section: Simulation Methodsmentioning

confidence: 99%

“…used dissipative particle dynamics (DPD) simulations to study the endocytosis of nanoparticles grafted with polyethylene glycol (PEG) polymers, and they found that increasing the surface density of ligands can benefit endocytosis. In addition to the DPD simulation method, coarse‐grained molecular dynamics (CGMD) simulation approaches have been developed and applied to investigate the receptor‐mediated endocytosis behaviour of nanoparticles . The various molecular potentials involved in CGMD methods are physically suitable for simulating biological systems, such as cell membranes.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Endocytosis behaviours of nanoparticles with helically decorated ligands

Guan

Wang

Lin

et al. 2019

Polymer International

Self Cite

View full text Add to dashboard Cite

In this work, the receptor‐mediated endocytosis of nanoparticles with helically decorated ligands was investigated by performing coarse‐grained molecular dynamics simulations. The results showed that a helical distribution of ligands can generate a unique spinning stage during endocytosis in which the nanoparticle is internalized with precession. This precession behaviour is primarily caused by a lateral torque acting on the nanoparticle. Compared with nanoparticles that present uniformly distributed ligands, nanoparticles with helically decorated ligands present an enhanced endocytosis capacity. Additionally, a longer spin duration and larger precessional angle were obtained by decreasing the helical period of the ligands. Increasing the aspect ratio of the nanoparticles strengthens their capacity to take the spinning endocytosis pathway. The present study not only reveals the mechanism underlying a new endocytosis pathway of nanoparticles with helically structured surfaces but also provides valuable theoretical information for the design of novel drug delivery systems that primarily occur via cellular uptake. © 2019 Society of Chemical Industry

show abstract

Section: Simulation Methodsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 99%

U_{bond} (r) = - \frac{1}{2} k_{normalb} r_{normalb}^{2} \ln [1 - {((), \frac{r}{r_{b}})}^{2}]

…”

Section: Simulation Methodsmentioning

confidence: 99%

“…The value of r a is set to be larger than 2 r b (3 σ ) so that the stiffness of lipids in the simulations can be maintained. This value was also adopted in some reported simulation work …”

Section: Simulation Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Endocytosis behaviours of nanoparticles with helically decorated ligands

Guan

Wang

Lin

et al. 2019

Polymer International

Self Cite

View full text Add to dashboard Cite

show abstract

Fluorinated Polyethylenimine to Enable Transmucosal Delivery of Photosensitizer‐Conjugated Catalase for Photodynamic Therapy of Orthotopic Bladder Tumors Postintravesical Instillation

Yuan

Deng

et al. 2019

Adv Funct Materials

111

View full text Add to dashboard Cite

Photodynamic therapy (PDT) by insertion of an optical fiber into the bladder cavity has been applied in the clinic for noninvasive treatment of bladder tumors. To avoid systemic phototoxicity, bladder intravesical instillation of a photosensitizer may be an ideal approach for PDT treatment of bladder cancer, in comparison to conventional intravenous injection. However, the instillationbased PDT for bladder cancer treatment remains to be less effective due to the poor urothelial uptake of photosensitizer, as well as the tumor hypoxiaassociated PDT resistance. Herein, it is uncovered that fluorinated polyethylenimine (F-PEI) achieved by mixing with Chorin-e6-conjugated catalase (CAT-Ce6) is able to form self-assembled CAT-Ce6/F-PEI nanoparticles, which show greatly improved cross-membrane, transmucosal, and intratumoral penetration capacities compared with CAT-Ce6 alone or nonfluorinated CAT-Ce6/PEI nanoparticles. Owing to the decomposition of tumor endogenous H 2 O 2 by CAT-Ce6/F-PEI nanoparticles penetrating into bladder tumors, the tumor hypoxia would be effectively relieved to further favor PDT. Therefore, bladder intravesical instillation with CAT-Ce6/F-PEI nanoparticles could offer remarkably improved photodynamic therapeutic effect to destruct orthotopic bladder tumors with reduced systemic toxicity compared to hematoporphyrin, the first-line photosensitizer used for bladder cancer PDT in clinic. This work presents a unique photosensitizer nanomedicine formulation, promising for clinical translation in instillation-based PDT to treat bladder tumors.

show abstract

Endocytosis‐Enabled Construction of Silica Nanochannels Crossing Living Cell Membrane for Transmembrane Drug Transport

Pan

Tang

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Artificial transmembrane channel (ATC) analogs are developed for overcoming biological membrane barriers and realizing transmembrane drug delivery, which are mostly studied within artificial lipid bilayers and thus lacked enough stability in practical applications on living cells. Here, natural endocytosis of silica‐based 1D nanomaterials (nanowires) with an ultrahigh aspect ratio is investigated. Enlightened by partially endocytosed ultralong silica nanowires, ATC that can penetrate living cell membranes for transmembrane transportation of small drug molecules is creatively constructed, resulting in enhanced drug delivery efficacy and decreased the half maximal inhibitory concentration. For the first time, an in‐depth study of the cellular uptake of 1D nanomaterials with ultrahigh aspect ratios (from 10 to 120) into living cells is carried out. Through confocal laser scanning microscopy observation, the endocytosis process of ultralong nanowires, including full uptake of short nanowires and partial uptake of longer nanowires, is clarified. Theoretical simulation is performed to give a fundamental understanding on the endocytosis mechanism of ultralong 1D silica nanowires. The simulation results demonstrate the time‐dependent internalization dynamics of the nanowires, which agrees well with our experimental results. This work not only clarifies the cellular interaction between 1D nanomaterials and living cells, but also pioneers the use of natural endocytosis of 1D nanomaterials for constructing ATC.

show abstract

Interaction Pathways between Plasma Membrane and Block Copolymer Micelles

Cited by 30 publications

References 59 publications

Endocytosis behaviours of nanoparticles with helically decorated ligands

Endocytosis behaviours of nanoparticles with helically decorated ligands

Fluorinated Polyethylenimine to Enable Transmucosal Delivery of Photosensitizer‐Conjugated Catalase for Photodynamic Therapy of Orthotopic Bladder Tumors Postintravesical Instillation

Endocytosis‐Enabled Construction of Silica Nanochannels Crossing Living Cell Membrane for Transmembrane Drug Transport

Contact Info

Product

Resources

About