Interaction of two oxysterols, 7-ketocholesterol and 25-hydroxycholesterol, with phosphatidylcholine and sphingomyelin in model membranes

Mintzer, Evan; Charles, Grace; Gordon, Sharon

doi:10.1016/j.chemphyslip.2010.05.001

Cited by 34 publications

(27 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such model membrane systems have been widely used to study the effects of cholesterol and cholesterol derivatives on monolayer behavior, and recently several groups have extended these experiments to oxysterol interactions. With cholesterol and most cholesterol derivatives, as the mole fraction of the sterol in the phospholipid monolayer increases, the mean molecular area decreases in a non-linear fashion [38, 46, 47]. The reduction in mean molecular area as compared to what would be expected from a mixture of two non-interacting components, indicates that these sterols condense membranes.…”

Section: Oxysterol Behavior In Model Membranesmentioning

confidence: 99%

Side-chain oxysterols: From cells to membranes to molecules

Olsen

Schlesinger

Ory

et al. 2012

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

This review discusses the application of cellular biology, molecular biophysics, and computational simulation to understand membrane-mediated mechanisms by which oxysterols regulate cholesterol homeostasis. Side-chain oxysterols, which are produced enzymatically in vivo, are physiological regulators of cholesterol homeostasis and primarily serve as cellular signals for excess cholesterol. These oxysterols regulate cholesterol homeostasis through both transcriptional and non-transcriptional pathways; however, many molecular details of their interactions in these pathways are still not well understood. Cholesterol trafficking provides one mechanism for regulation. The current model of cholesterol trafficking regulation is based on the existence of two distinct cholesterol pools in the membrane: a low and a high availability/activity pool. It is proposed that the low availability/activity pool of cholesterol is integrated into tightly packing phospholipids and relatively inaccessible to water or cellular proteins, while the high availability cholesterol pool is more mobile in the membrane and is present in membranes where the phospholipids are not as compressed. Recent results suggest that that oxysterols may promote cholesterol egress from membranes by shifting cholesterol from the low to the high activity pools. Furthermore, molecular simulations suggest a potential mechanism for oxysterol “activation” of cholesterol through its displacement in the membrane. This review discusses these results as well as several other important interactions between oxysterols and cholesterol in cellular and model lipid membranes.

show abstract

Section: Oxysterol Behavior In Model Membranesmentioning

confidence: 99%

Side-chain oxysterols: From cells to membranes to molecules

Olsen

Schlesinger

Ory

et al. 2012

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

show abstract

“…There is a limited amount of experimental [42,43] and computational [44][45][46] data describing the molecular details of oxysterol interactions with other constituents of mammalian cell membranes.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol under oxidative stress—How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Kulig

Olżyńska

Jurkiewicz

et al. 2015

Free Radical Biology and Medicine

100

View full text Add to dashboard Cite

“…This indicates that 7keto mainly affected Ld phase, rendering it more fluid and the oxysterol did not influence the fluidity of Lo phase. The ability of 7keto to increase membrane fluidity may involve in its orientation in lipid bilayer which adopts a tilt with respect to membrane surface so that both oxygen groups are exposed to the hydrophilic interface, thus decreasing membrane lipid packing [23].…”

Section: The Effect Of Cholesterol and 7keto On The Fluidity Of Membrmentioning

confidence: 99%

“…7keto is the major product of cholesterol oxidation induced by reactive oxygen species [22] and is shown to significantly affect membrane properties and interaction with Ab [23][24][25]. We employed a heterogeneous cell-sized liposome system, composed of cholesterol, equal concentrations of unsaturated phospholipid and saturated phospholipid.…”

Section: Introductionmentioning

confidence: 99%

Localization of amyloid beta (Aβ1‐42) protofibrils in membrane lateral compartments: Effect of cholesterol and 7‐Ketocholesterol

et al. 2014

View full text Add to dashboard Cite

a b s t r a c tCholesterol plays an important role in the interaction of Alzheimer's amyloid beta (Ab) with cell membranes, an important event in Ab-induced cytotoxicity. However, it is not fully understood how cholesterol influences the association of Ab with membrane lateral compartments. We have shown that by modulating membrane fluidity, cholesterol decreased peptide localization in solidordered domains and increased that in liquid-ordered domains. It changed the amount of Ab associating with liquid-disordered (Ld) phase with different tendencies depending on the composition of heterogeneous membrane systems. 7-Ketocholesterol, an oxidized derivative of cholesterol, majorly enhanced the fluidity of and Ab interaction with Ld phase. These findings are useful for clarifying the impact of cholesterol and its oxidation in Ab-induced toxicity.

show abstract

Interaction of two oxysterols, 7-ketocholesterol and 25-hydroxycholesterol, with phosphatidylcholine and sphingomyelin in model membranes

Cited by 34 publications

References 59 publications

Side-chain oxysterols: From cells to membranes to molecules

Side-chain oxysterols: From cells to membranes to molecules

Cholesterol under oxidative stress—How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

Localization of amyloid beta (Aβ1‐42) protofibrils in membrane lateral compartments: Effect of cholesterol and 7‐Ketocholesterol

Contact Info

Product

Resources

About