Interaction of the synaptic protein PICK1 (protein interacting with C kinase 1) with the non-voltage gated sodium channels BNC1 (brain Na+ channel 1) and ASIC (acid-sensing ion channel)

Hruska-Hageman, Alesia M.; Wemmie, John A.; Price, Margaret P.; Welsh, Michael J.

doi:10.1042/bj3610443

Cited by 78 publications

(15 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, whether ASIC1a and ASIC2a are distributed in the growth cones of immature neurons during development is not known. In addition, whether ASIC1a and ASIC2a are presynaptically localized in mature neurons is controversial (Hruska-Hageman et al, 2002;Alvarez de la Rosa et al, 2003;Zha et al, 2006). In this study, we demonstrated that the ASIC1a and ASIC2a subunits are present in axonal growth cones from 3 days in vitro (DIV3) to DIV6 hippocampal neurons.…”

Section: Introductionmentioning

confidence: 73%

Distribution of Acid Sensing Ion Channels in Axonal Growth Cones and Presynaptic Membrane of Cultured Hippocampal Neurons

Liu¹,

Liu²,

Ye³

et al. 2020

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Although acid-sensing ion channels (ASICs) are widely expressed in the central nervous system, their distribution and roles in axonal growth cones remain unclear. In this study, we examined ASIC localization and function in the axonal growth cones of cultured immature hippocampal neurons. Our immunocytochemical data showed that native and overexpressed ASIC1a and ASIC2a are both localized in growth cones of cultured young hippocampal neurons. Calcium imaging and electrophysiological assay results were utilized to validate their function. The calcium imaging test results indicated that the ASICs (primarily ASIC1a) present in growth cones mediate calcium influx despite the addition of voltage-gated Ca 2+ channels antagonists and the depletion of intracellular calcium stores. The electrophysiological tests results suggested that a rapid decrease in extracellular pH at the growth cones of voltage-clamped neurons elicits inward currents that were blocked by bath application of the ASIC antagonist amiloride, showing that the ASICs expressed at growth cones are functional. The subsequent immuno-colocalization test results demonstrated that ASIC1a and ASIC2a are both colocalized with Neurofilament-H and Bassoon in mature hippocampal neurons. This finding demonstrated that after reaching maturity, ASIC1a and ASIC2a are both distributed in axons and the presynaptic membrane. Our data reveal the distribution of functional ASICs in growth cones of immature hippocampal neurons and the presence of ASICs in the axons and presynaptic membrane of mature hippocampal neurons, indicating a possible role for ASICs in axonal guidance, synapse formation and neurotransmitter release.

show abstract

Section: Introductionmentioning

confidence: 73%

Distribution of Acid Sensing Ion Channels in Axonal Growth Cones and Presynaptic Membrane of Cultured Hippocampal Neurons

Liu¹,

Liu²,

Ye³

et al. 2020

Front. Cell. Neurosci.

View full text Add to dashboard Cite

show abstract

“…In addition to interacting with GluR2, PICK1 interacts specifically with the C-termini of ASIC1 and ASIC2 in central neurons via its PDZ domain [23,24]. ASIC1 is expressed predominantly in small-diameter DRG neurons, whereas ASIC2 is localized to the plasma membrane of small-, medium-, and large-diameter cells of the DRG [25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Preserved Acute Pain and Impaired Neuropathic Pain in Mice Lacking Protein Interacting with C Kinase 1

et al. 2011

View full text Add to dashboard Cite

Protein interacting with C Kinase 1 (PICK1), a PDZ domain-containing scaffolding protein, interacts with multiple different proteins in the mammalian nervous system and is believed to play important roles in diverse physiological and pathological conditions. In this study, we report that PICK1 is expressed in neurons of the dorsal root ganglion (DRG) and spinal cord dorsal horn, two major pain-related regions. PICK1 was present in approximately 29.7% of DRG neurons, most of which were small-less than 750 μm2 in cross-sectional area. Some of these PICK1-positive cells co-labeled with isolectin B4 or calcitonin-gene-related peptide. In the dorsal horn, PICK1 immunoreactivity was concentrated in the superficial dorsal horn, where it was prominent in the postsynaptic density, axons, and dendrites. Targeted disruption of PICK1 gene did not affect basal paw withdrawal responses to acute noxious thermal and mechanical stimuli or locomotor reflex activity, but it completely blocked the induction of peripheral nerve injury-induced mechanical and thermal pain hypersensitivities. PICK1 appears to be required for peripheral nerve injury-induced neuropathic pain development and to be a potential biochemical target for treating this disorder.

show abstract

“…That conclusion is consistent with previous studies in brain slices and cultured neurons that localized ASIC1 and ASIC2 at dendritic spines Zha et al 2006Zha et al , 2009). In addition, ASIC1 and ASIC2 were enriched in synaptosomal membrane preparations Zha et al 2009) and associated with postsynaptic scaffolding proteins (Duggan et al 2002;Hruska-Hageman et al 2002;Xu & Xiong 2007;Zha et al 2009). Thus, the data suggest that ASIC1 and ASIC2 sit in the postsynaptic membrane.…”

Section: Asic Channels Influence Behavior In Response To Intense Avementioning

confidence: 99%

Localization and behaviors in null mice suggest that ASIC1 and ASIC2 modulate responses to aversive stimuli

Price

Gong

Parsons

et al. 2013

Genes Brain and Behavior

View full text Add to dashboard Cite

Acid sensing ion channels (ASICs) generate H+-gated Na+ currents that contribute to neuronal function and animal behavior. Like ASIC1, ASIC2 subunits are expressed in the brain and multimerize with ASIC1 to influence acid-evoked currents and facilitate ASIC1 localization to dendritic spines. To better understand how ASIC2 contributes to brain function, we localized the protein and tested the behavioral consequences of ASIC2 gene disruption. For comparison, we also localized ASIC1 and studied ASIC1−/− mice. ASIC2 was prominently expressed in areas of high synaptic density, and with a few exceptions, ASIC1 and ASIC2 localization exhibited substantial overlap. Loss of ASIC1 or ASIC2 decreased freezing behavior in contextual and auditory cue fear conditioning assays, in response to predator odor, and in response to CO2 inhalation. In addition, loss of ASIC1 or ASIC2 increased activity in a forced swim assay. These data suggest that ASIC2, like ASIC1, plays a key role in determining the defensive response to aversive stimuli. They also raise the question of whether gene variations in both ASIC1 and ASIC2 might affect fear and panic in humans.

show abstract

Interaction of the synaptic protein PICK1 (protein interacting with C kinase 1) with the non-voltage gated sodium channels BNC1 (brain Na+ channel 1) and ASIC (acid-sensing ion channel)

Cited by 78 publications

References 42 publications

Distribution of Acid Sensing Ion Channels in Axonal Growth Cones and Presynaptic Membrane of Cultured Hippocampal Neurons

Distribution of Acid Sensing Ion Channels in Axonal Growth Cones and Presynaptic Membrane of Cultured Hippocampal Neurons

Preserved Acute Pain and Impaired Neuropathic Pain in Mice Lacking Protein Interacting with C Kinase 1

Localization and behaviors in null mice suggest that ASIC1 and ASIC2 modulate responses to aversive stimuli

Contact Info

Product

Resources

About