Interaction of the nematophagous fungusDuddingtonia flagransonAmblyomma cajannenseengorged females and enzymatic characterisation of its chitinase

Braga, Fábio Ribeiro; Araújo, Jackson Víctor de; Soares, Filippe Elias de Freitas; Araújo, Juliana Milani; Tavela, Alexandre de Oliveira; Carvalho, Lorendane Millena de; Mello, Ingrid Ney Kramer de; Paula, Alessandra Teixeira de; Lelis, Rosane Teixeira; Queiróz, José Humberto de

doi:10.1080/09583157.2013.789481

Cited by 20 publications

(16 citation statements)

References 34 publications

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“…Each vial was covered with hydrophobic cotton and autoclaved at 121°C for 15 min. After cooling, each flask was inoculated with the conidial suspension previously prepared and incubated in a BOD incubator at 28°C for five days (Braga et al, 2013). After this period, chitinase produced was extracted according to the method of Soares et al (2010).…”

Section: Solid State Fermentationmentioning

confidence: 99%

“…Chitinase activity was measured through the measure of the reducing sugars using DNS (3,5-dinitrosalicylic acid) method (Miller, 1959). The assay was composed of: 10 µL of enzyme, 40 µL of sodium acetate buffer 50 mM pH 5.5 and 50 µL of 1% colloidal chitin previously prepared according to the method of Braga et al (2013), incubated at 50°C. The reaction was stopped by addition of 100 µL of DNS reagent.…”

Section: Enzymatic Activitymentioning

confidence: 99%

“…In general biological terms, this polymer is essential for body constitution of a number of organisms and, among these are: cuticles and membranes of insects, cell walls of fungi and helminth eggshells (Gortari and Hours, 2008). Moreover, it has been demonstrated that the destruction of the exoskeleton of arthropods can be done in a biological way by using chitinases that certain fungi can produce (St et al, 1996;Bogus et al, 2005;Braga et al, 2013). Purified proteases and chitinases demonstrate nematicidal activity against gastrointestinal nematodes of domestic animals (Tikhonov et al, 2002;Braga et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Statistical screening for the chitinase production by nematophagous fungi from Monacrosporium genu

Filippe¹,

Jose²,

Jackson³

et al. 2015

Afr. J. Microbiol. Res.

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Several chitinases with nematicidal action of these fungi have been identified in recent years, however, little is known about which nutritional factors from the culture medium are significant for its mass production. The aim of this study was to perform the statistical screening of the components of the culture medium for chitinase production by nematophagous fungi, Monacrosporium sinense (SF53) and Monacrosporium thaumasium (NF34) aiming to identify which of these components were significant and to evaluate the influence of pH on chitinase activity of these fungi. The effect of five variables (chitin, colloidal chitin, glucose, sodium nitrate and moisture) on chitinase production was analyzed using Plackett-Burman statistical design. Chitinase activity was determined at different pH (pH 3.5, 4.5 and 5.5) and Tris-HCl 50 mM (pH 7.0 and 8.0). In relation to the isolate NF34, at the levels evaluated, two variables were significant (p <0.05) for chitinase production: chitin and NaNO 3. On the other hand, for SF53, at the levels evaluated, only glucose was significant (p <0.05). Regarding the influence of pH on enzyme activity for M. thaumasium the pH value with the highest activity obtained was 5.5. However, for M. sinense, the highest activity was observed at pH 8. These data demonstrate that different species of nematophagous fungi may exhibit better enzymatic activities and consequently, better predatory activities at different environmental conditions.

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Section: Solid State Fermentationmentioning

confidence: 99%

Section: Enzymatic Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Statistical screening for the chitinase production by nematophagous fungi from Monacrosporium genu

Filippe¹,

Jose²,

Jackson³

et al. 2015

Afr. J. Microbiol. Res.

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“…Therefore, its applicability has been studied extensively. [19][20][21][22] This study evaluates a new approach to the application of these organisms, involving the biosynthesis of AgNPs, since to date, there are few reports on the use of nematophagous fungi in the synthesis of NPs. 23 However, it is worth noting that there is no scientific report of the production of AgNPs using D. flagrans.…”

Section: Costa Silva Et Almentioning

confidence: 99%

“…This occurs because the D. flagrans fungus can be stimulated to produce hydrolytic enzymes such as protease, pectinase, phospholipase, and chitinase according to the constituents of the medium. 20,22,28 In this study, the medium was enriched with a natural source of chitin (tick shells) in order to increase the production of chitinase. Braga et al 22 reported that the chitinase produced by the D. flagrans fungus has a weight of 34 kDa and maximum activity at pH 8-10 and 60°C.…”

Section: Analysis Of Cell-free Fungal Filteredmentioning

confidence: 99%

Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans

Silva

Oliveira

Keijok

et al. 2017

IJN

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The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the extracellular synthesis of highly stable silver NPs (AgNPs) using the nematophagous fungus Duddingtonia flagrans (AC001). The fungal cell-free filtrate was analyzed by the Bradford method and 3,5-dinitrosalicylic acid assay and used to synthesize the AgNPs in the presence of a 1 mM AgNO 3 solution. They have been characterized by UV–Vis spectroscopy, X-ray diffraction, transmission electron microscopy, dynamic light scattering, Zeta potential measurements, Fourier-transform infrared, and Raman spectroscopes. UV–Vis spectroscopy confirmed bioreduction, while X-ray diffractometry established the crystalline nature of the AgNPs. Dynamic light scattering and transmission electron microscopy images showed approximately 11, 38 nm monodisperse and quasispherical AgNPs. Zeta potential analysis was able to show a considerable stability of AgNPs. The N–H stretches in Fourier-transform infrared spectroscopy indicate the presence of protein molecules. The Raman bands suggest that chitinase was involved in the growth and stabilization of AgNPs, through the coating of the particles. Our results show that the NPs we synthesized have good stability, high yield, and monodispersion.

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Interaction of the nematophagous fungus Pochonia chlamydosporia and Parascaris equorum eggs in different culture media

Carvalho

Braga

Domingues

et al. 2013

J. Basic Microbiol.

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Research involving the use of nematophagous fungi in the biological control of parasites of interest to veterinarians has occurred over recent years, with promising results. This article reports the infection of Parascaris equorum eggs by the fungus Pochonia chlamydosporia (isolates VC1 and VC4). Six groups were formed for each isolate, with six different culture media: 2% water-agar (2% WA); agar-chitin (AC); YPSSA (yeast extract, K2HPO4, MgSO4 ·7H2O, soluble starch); AELA extract (starch + water + agar); 2% corn-meal-agar (2% CMA); and 2% potato dextrose-agar (2% PDA). A total of 1000 eggs of P. equorum were transferred to each plate containing isolates grown for a period of 7 days (treatment group). Also, 1000 eggs were added to each plate without fungus (controlgroup). The plates were kept in an environmental chamber at 25 °C in the dark for 21 days. After, we analyzed the effects on ovicidal activity: effect 1 (accession shell); effect 2 (penetration hyphae); and effect 3 (destruction of the eggs). No differences were observed in the destruction of eggs between the two isolates. The decreasing effectiveness of the different culture media was: PDA (38.9%); CMA (38.3%); WA (36.7%); YPSSA (36.45%); and AC (32.5%). The highest percentage egg destruction was observed when the strains were grown in culture medium AELA (44.9%); this was the best medium.

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Interaction of the nematophagous fungusDuddingtonia flagransonAmblyomma cajannenseengorged females and enzymatic characterisation of its chitinase

Cited by 20 publications

References 34 publications

Statistical screening for the chitinase production by nematophagous fungi from Monacrosporium genu

Statistical screening for the chitinase production by nematophagous fungi from Monacrosporium genu

Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus <em>Duddingtonia flagrans</em>

Interaction of the nematophagous fungus Pochonia chlamydosporia and Parascaris equorum eggs in different culture media

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