Interaction of the maturation protein of the bacteriophage MS2 and the sex pilus of the Escherichia coli F plasmid

Spankie, Timothy J.; Haywood, Alexe L.; Dottorini, Tania; Barrow, Paul; Hirst, Jonathan D.

doi:10.1016/j.jmgm.2020.107723

Cited by 2 publications

(3 citation statements)

References 48 publications

(51 reference statements)

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“…This can cause plasmid loss and result in antibiotic sensitivity, as many plasmids carry antibiotic resistance determinants [25][26][27][28] . Bacteria-plasmid-phage interactions have been primarily studied in laboratory settings and are yet to be thoroughly tested in complex communities.…”

Section: Background (4901 Words)mentioning

confidence: 99%

“…In contrast, phage may target plasmid components, such as the conjugal apparatus plasmids use to transfer copies of themselves 23–25 . This can cause plasmid loss and result in antibiotic sensitivity, as many plasmids carry antibiotic resistance determinants 25–28 . Bacteria-plasmid-phage interactions have been primarily studied in laboratory settings and are yet to be thoroughly tested in complex communities.…”

Section: Introductionmentioning

confidence: 99%

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Urinary F plasmids reduce permissivity to coliphage infection

Hernandez

Putonti

Wolfe

2022

Preprint

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The microbial community of the urinary tract (urinary microbiota or urobiota) has been associated with human health. Bacteriophages (phages) and plasmids present in the urinary tract, like in other niches, may shape urinary bacteria dynamics. While urinary E. coli, often associated with urinary tract infection (UTI), and their phages have been catalogued for the urobiome, the dynamics of their interactions have yet to be explored. In this study, we characterized urinary E. coli F plasmids and their ability to decrease permissivity to E. coli phage (coliphage) infection. Putative F plasmids were present in 47 of 67 urinary E. coli isolates, and most of these plasmids carry genes that encode for toxin-antitoxin (TA) modules, antibiotic resistance, and/or virulence. Two urinary E. coli F plasmids, from urinary microbiota (UMB) 0928 and 1284, were conjugated into E. coli K-12 strains; plasmids included genes for antibiotic resistance and virulence. These plasmids, pU0928 and pU1284, decreased permissivity to coliphage infection by the laboratory phage P1vir and the urinary phages Greed and Lust. Furthermore, pU0928 could be maintained in E. coli K-12 for up to ten days in the absence of antibiotic resistance selection; this included maintenance of the antibiotic resistance phenotype and decreased permissivity to phage. Finally, we discuss how F plasmids present in urinary E. coli could play a role in coliphage dynamics and maintenance of antibiotic resistance in urinary E. coli.

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Section: Background (4901 Words)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Urinary F plasmids reduce permissivity to coliphage infection

Hernandez

Putonti

Wolfe

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In contrast, phages may target plasmid components such as the conjugal apparatus plasmids used to transfer copies of themselves ( 23 – 25 ). This can cause plasmid loss and result in antibiotic sensitivity as many plasmids carry antibiotic resistance determinants ( 23 , 25 – 27 ). Bacterium-plasmid-phage interactions have been studied primarily in laboratory settings and are yet to be thoroughly tested in complex communities.…”

Section: Introductionmentioning

confidence: 99%