Interaction of the lipid and protein components of pulmonary surfactant Role of phosphatidylglycerol and calcium

King, Richard J.; MacBeth, Mary Catherine

doi:10.1016/0005-2736(81)90242-x

Cited by 128 publications

(50 citation statements)

References 16 publications

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“…Although apoprotein acts to increase the adsorption of natural pulmonary surfactant (eg, References 4,[8][9][10][11][12][13][14], lipid extracts with almost an order of magnitude less protein can be formulated in vitro to exhibit the same rapid adsorption properties (4, 8, 15-1 8). Such extracts have excellent dynamic surface tension lowering characteristics similar to natural lung surfactant (1 5, 17, 18).…”

Section: Vei Ventilator Efficiency Index Vp Volume Pressurementioning

confidence: 99%

Lung Surfactant Replacement in Premature Lambs with Extracted Lipids from Bovine Lung Lavage: Effects of Dose, Dispersion Technique, and Gestational Age

et al. 1985

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Section: Vei Ventilator Efficiency Index Vp Volume Pressurementioning

confidence: 99%

Lung Surfactant Replacement in Premature Lambs with Extracted Lipids from Bovine Lung Lavage: Effects of Dose, Dispersion Technique, and Gestational Age

et al. 1985

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“…The apoproteins present in this complex have been the subject of many studies over the past few years which have helped to elucidate the physiologic role they play in surfactant activity. While initial interest seemed focused primarily on a 35,000-D major glycoprotein (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17), more recent studies have described hydrophobic 5,000-18,000-D apoproteins (18)(19)(20)(21)(22)(23)(24)(25)(26)(27) Revak. Receivedfor publication 25 June 1987 and in revisedform 28 September 1987. expression of surfactant surface tension reduction activity.…”

Section: Introductionmentioning

confidence: 99%

Use of human surfactant low molecular weight apoproteins in the reconstitution of surfactant biologic activity.

Revak

Merritt²,

Degryse³

et al. 1988

J. Clin. Invest.

134

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Two low molecular weight (LMW) apoproteins were isolated from human pulmonary surfactant. SDS polyacrylamide gel analysis showed one protein (SP 18) to have an apparent molecular weight of 18,000 when unreduced and 9,000 D after reduction. The second protein (SP 9) migrated at -9,000 D in the presence or absence of reducing agents. Both proteins contain a high number of hydrophobic amino acids. The NH2-terminal sequence of SP 18 was determined to be: NH2-phe-proile-pro-leu-pro-tyr-. A cDNA clone isolated from a human adult lung cDNA library contained a long open reading frame encoding at an internal position the human SP 18 amino-terminal sequence.Mixtures of phospholipids (PL) and SP 9 and SP 18 were assessed for their capacity to reduce surface tensions on a pulsating bubble surfactometer. The addition of 1% apoprotein resulted in a reduction of surface tension after 15 s from 42.9 dyn/cm for PL alone to 16.7 and 6.3 dyn/cm for preparations containing SP 9 and SP 18, respectively. In vivo assessment of reconstituted surfactant activity was performed in fetal rabbits. Reconstituted surfactant consisting of PL + 0.5% SP 18 instilled intratracheally at delivery resulted in a marked increase in lung compliance, while the incorporation of 0.5% SP 9 yielded a moderate increase. These data show the ability to produce biologically active surfactant by the addition of isolated LMW apoproteins to defined PL.

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“…However, we think the action of an inhibitor or inactivator is not a likely explanation for the alveolar instability because of the rapidity with, which a large inflation reverses the increased aggregation (5,6) and the decreased compliance (1, 2) that develop during low volume ventilation. We do not know if the altered surface properties (6) reflect the physical state or chemical composition of the material that pellets at low centrifugal forces (16).…”

mentioning

confidence: 99%

Surfactant deficiency in rats without a decreased amount of extracellular surfactant.

Massaro¹,

Clerch²,

Temple³

et al. 1983

J. Clin. Invest.

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A B S T R A C T Low volume ventilation without periodic large inflations leads to diminished alveolar stability and to the accumulation of increased amounts of airway disaturated phosphatidylcholine (DSPC) in large aggregates that sediment at 1,000 g; surfactant in this form lowers surface tension less rapidly than surfactant present in the 1,000-g supernatant fraction.These observations led to the present work in which we tested the notion that alveolar instability may develop in the presence of an undiminished quantity of total airway surfactant, if the amount of surfactant found in the 1,000-g supernatant fraction is diminished.Pulmonary compliance fell and the alveolar-arterial 02 gradient widened in normothermic rats during constant ventilation in the resting tidal volume range, and, in hyperthermic rats (-390C) similarly ventilated but with the addition of periodic sighs. The total amount of airway DSPC was undiminished in each group, but in each less DSPC was present in the 1,000-g supernatant fraction compared with controls. Alveolar instability and hypoxemia also developed in hyperthermic rats during low volume ventilation without periodic sighs. Although the total amount of airway DSPC was decreased in these rats, enough remained to theoretically form a continuous monomolecular film over the entire alveolar surface at functional residual capacity; however, there was insufficient surfactant in This work was presented in part at

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Interaction of the lipid and protein components of pulmonary surfactant Role of phosphatidylglycerol and calcium

Cited by 128 publications

References 16 publications

Lung Surfactant Replacement in Premature Lambs with Extracted Lipids from Bovine Lung Lavage: Effects of Dose, Dispersion Technique, and Gestational Age

Lung Surfactant Replacement in Premature Lambs with Extracted Lipids from Bovine Lung Lavage: Effects of Dose, Dispersion Technique, and Gestational Age

Use of human surfactant low molecular weight apoproteins in the reconstitution of surfactant biologic activity.

Surfactant deficiency in rats without a decreased amount of extracellular surfactant.

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