Interaction of the Anti-Inflammatory Annexin A1 Protein and Tacrolimus Immunosuppressant in the Renal Function of Rats

Araújo, Leandro; Truzzi, Renata R.; Mendes, Glória Elisa Florido; Luz, Marcus Alexandre Mendes; Burdmann, Emmanuel A.; Oliani, Sônia Maria

doi:10.1159/000309756

Cited by 14 publications

(17 citation statements)

References 41 publications

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“…Similarly, we have shown that salt-depleted tacrolimus treatment augments renal ANXA1 expression [25]. ANXA1 expression has previously been shown to increase in response to glucocorticoids [38], inflammation [28], heat or chemical cellular damage [39] and proinflammatory cytokines [40].…”

Section: Discussionsupporting

confidence: 59%

“…After anesthesia, rats underwent renal hemodynamic studies as previously described [25]. RBF measurements were taken (T 106, Transonic Systems Inc., NY, USA) during 40 min.…”

Section: Renal Blood Flowmentioning

confidence: 99%

“…Renal sections (4 lm) underwent immunohistochemical staining [25]. They were incubated overnight at 4°C with 1:1,000 monoclonal mouse anti-rat-ED-1 (Serotec, Oxford, UK) or 1:2,000 polyclonal rabbit anti-ANXA1 (Zymed Laboratories, Cambridge, UK).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…There is much evidence demonstrating the importance and protective role of ANXA1 in cardiac, mesenteric, cerebral and renal ischemia/reperfusion (I/R) injury [20][21][22][23][24]. Recently, we demonstrated a structural protective role for ANXA1 in an experimental model of acute tacrolimus nephrotoxicity [25].…”

Section: Introductionmentioning

confidence: 99%

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Annexin A1 protein attenuates cyclosporine-induced renal hemodynamics changes and macrophage infiltration in rats

et al. 2011

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Background Cyclosporine (CsA) remains an important immunosuppressant for transplantation and for treatment of autoimmune diseases. The most troublesome side effect of CsA is renal injury. Acute CsA-induced nephrotoxicity is characterized by reduced renal blood flow (RBF) and glomerular filtration rate (GFR) due to afferent arteriole vasoconstriction. Annexin A1 (ANXA1) is a potent antiinflammatory protein with protective effect in renal ischemia/reperfusion injury. Here we study the effects of ANXA1 treatment in an experimental model of acute CsA nephrotoxicity. Methods Salt-depleted rats were randomized to treatment with VH (vehicles 1 mL/kg body weight/day), ANXA1 (Ac2-26 peptide 1 mg/kg body weight/day intraperitoneally), CsA (20 mg/kg body weight/day subcutaneously) and CsA ? ANXA1 (combination) for seven days. We compared renal function and hemodynamics, renal histopathology, renal tissue macrophage infiltration and renal ANXA1 expression between the four groups. Results CsA significantly impaired GFR and RBF, caused tubular dilation and macrophage infiltration and increased ANXA1 renal tissue expression. Treatment with ANXA1 attenuated CSA-induced hemodynamic changes, tubular injury and macrophage infiltration. Conclusion ANXA1 treatment attenuated renal hemodynamic injury and inflammation in an acute CsA nephrotoxicity model.

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Section: Discussionsupporting

confidence: 59%

“…After anesthesia, rats underwent renal hemodynamic studies as previously described [25]. RBF measurements were taken (T 106, Transonic Systems Inc., NY, USA) during 40 min.…”

Section: Renal Blood Flowmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Annexin A1 protein attenuates cyclosporine-induced renal hemodynamics changes and macrophage infiltration in rats

et al. 2011

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“…Ubd, which encodes a protein called Fat10, has been shown to be a pro-apoptotic and pro-inflammatory protein in HIVAN [16]. Interestingly, Anxa1 has been reported to display anti-inflammatory effects [17]. Spon1 is a novel candidate gene for hypertension [18].…”

Section: Resultsmentioning

confidence: 99%

Temporal Profile of the Renal Transcriptome of HIV-1 Transgenic Mice during Disease Progression

et al. 2014

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Profiling of temporal changes of gene expression in the same kidney over the course of renal disease progression is challenging because repeat renal biopsies are rarely indicated in clinical practice. Here, we profiled the temporal change in renal transcriptome of HIV-1 transgenic mice (Tg26), an animal model for human HIV-associated nephropathy (HIVAN), and their littermates at three different time points (4, 8, and 12 weeks of age) representing early, middle, and late stages of renal disease by serial kidney biopsy. We analyzed both static levels of gene expression at three stages of disease and dynamic changes in gene expression between different stages. Analysis of static and dynamic changes in gene expression revealed that up-regulated genes at the early and middle stages are mostly involved in immune response and inflammation, whereas down-regulated genes mostly related to fatty acid and retinoid metabolisms. We validated the expression of a selected panel of genes that are up-regulated at the early stage (CCL2, CCL5, CXCL11, Ubd, Anxa1, and Spon1) by real-time PCR. Among these up-regulated genes, Spon1, which is a previously identified candidate gene for hypertension, was found to be up-regulated in kidney of human with diabetic nephropathy. Immunostaining of human biopsy samples demonstrated that protein expression of Spon1 was also markedly increased in kidneys of patients with both early and late HIVAN and diabetic nephropathy. Our studies suggest that analysis of both static and dynamic changes of gene expression profiles in disease progression avails another layer of information that could be utilized to gain a more comprehensive understanding of disease progression and identify potential biomarkers and drug targets.

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Association between miRNA‐196a2 rs11614913 T>C polymorphism and Kawasaki disease susceptibility in southern Chinese children

Qiu

Zeng

et al. 2019

Clinical Laboratory Analysis

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This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. AbstractBackground: miRNAs play important roles in a variety of diseases. Thus, the association between miRNA-196a2 rs11614913 T>C polymorphism and Kawasaki disease susceptibility is still unknown. Methods:We included 532 children with Kawasaki disease and 623 healthy children from South China, and their DNA was extracted for genotyping by TaqMan methodology. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to estimate the strength of association. Results: No significant associations were observed between the miRNA-196a2rs11614913 T>C polymorphisms and Kawasaki disease risk (TC vs TT: adjusted OR = 1.04, 95% CI = 0.79-1.37; CC vs TT: adjusted OR = 0.87, 95% CI = 0.63-1.21; dominant model: adjusted OR = 0.99, 95% CI = 0.76-1.27; and recessive model: adjusted OR = 0.85, 95% CI = 0.64-1.13). There was also no significant correlation found in stratified analyses. Conclusion:This study suggests that miRNA-196a2 rs11614913 T>C may not be associated with Kawasaki disease susceptibility in a southern Chinese population. Larger, multicenter studies are needed to confirm our conclusions. K E Y W O R D SKawasaki disease, miRNA-196a2, polymorphism, susceptibility 2 of 6 | WANG et Al.

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Interaction of the Anti-Inflammatory Annexin A1 Protein and Tacrolimus Immunosuppressant in the Renal Function of Rats

Cited by 14 publications

References 41 publications

Annexin A1 protein attenuates cyclosporine-induced renal hemodynamics changes and macrophage infiltration in rats

Annexin A1 protein attenuates cyclosporine-induced renal hemodynamics changes and macrophage infiltration in rats

Temporal Profile of the Renal Transcriptome of HIV-1 Transgenic Mice during Disease Progression

Association between miRNA‐196a2 rs11614913 T>C polymorphism and Kawasaki disease susceptibility in southern Chinese children

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