Interaction of TGF-β4 and IL-17 with IgA secretion in the intestine of chickens fed with E. faecium AL41 and challenged with S. Enteritidis

Karaffová, Viera; Bobíková, Katarína; Husáková, Eva; Levkut, M.; Herich, R.; Révajová, Viera; Levkutová, M.; Levkut, M.

doi:10.1016/j.rvsc.2015.04.005

Cited by 29 publications

(19 citation statements)

References 25 publications

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“…Previous studies have shown that probiotic strains of E. faecium (EF55 and AL41) increased the number of IgA+ cells in the intestine of chickens at a late phase of infection with Salmonella Enteritidis Karaffová et al, 2015). Similarly, our experiment demonstrated that the highest density of IgA+ cells was found in the groups treated with E. faecium AL41 (EFAL41, EFAL41 + CJ) at 7 dpi also in the case of infection with C. jejuni.…”

Section: Discussionsupporting

confidence: 84%

Influence of oral application of Enterococcus faecium AL41 on TGF-β4 and IL-17 expression and immunocompetent cell distribution in chickens challenged with Campylobacter jejuni

Letnická

Karaffová

Levkut

et al. 2017

Acta Veterinaria Hungarica

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Campylobacteriosis is mainly caused by infection with Campylobacter jejuni following consumption or handling of Campylobacter-contaminated poultry meat. The aim of this study was to investigate the effect of probiotic Enterococcus faecium AL41 on TGF-β4 and IL-17 expression and on immunocompetent cell distribution after C. jejuni infection in broiler chicken, as a second part of the previous study of Karaffová et al. (2017). Accordingly, day-old chicks were randomly divided into four experimental groups of 10 chicks each (n = 10): control (C), E. faecium AL41 (EFAL41), C. jejuni CCM6191 (CJ), and combined E. faecium AL41 + C. jejuni CCM6191 (EFAL41 + CJ). Samples from the caecum were collected on days 4 and 7 post Campylobacter infection (dpi), for the isolation of mRNA of TGF-β4, IL-17 and for immunohistochemistry. The relative mRNA expression of TGF-β4 was upregulated in the combined (EFAL41 + CJ) group compared to other groups during both samplings, but the expression of IL-17 was downregulated. Similarly, the highest density of CD3+ was detected in the combined group at 7 dpi, but the number of IgA+ cells was increased in both groups with EFAL41. It was concluded that the EFAL41 probiotic E. faecium strain can modulate the expression of selected cytokines (upregulation of TGF-β4 but downregulation of IL-17 relative expression), and activate IgA-producing cells in the caeca of chicks infected with C. jejuni CCM6191.

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Section: Discussionsupporting

confidence: 84%

Influence of oral application of Enterococcus faecium AL41 on TGF-β4 and IL-17 expression and immunocompetent cell distribution in chickens challenged with Campylobacter jejuni

Letnická

Karaffová

Levkut

et al. 2017

Acta Veterinaria Hungarica

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“…Both bareback-riding camps in our study provided foraging opportunities during tourist activities, which might mitigate stress [ 69 , 70 ], but higher IgA potentially related to better welfare was only observed in Camp C. As with FGM, management factors other than or perhaps in addition to riding likely play a role in IgA production, so it is unclear if higher IgA would be a sign of positive welfare and healthy immune function in this context. Ultimately using IgA as a welfare marker is complicated by the fact that chronic stressors also can suppress immune function and reduce IgA production [ 25 , 28 ], while acute illnesses that elicit immune responses to cope with underlying pathology can increase it [ 24 , 71 , 72 ]. Thus, interpretation of IgA measures, just like GCs, is not always straightforward, as both can increase in response to acute stressors of a non-immune nature [ 27 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Tourist Activities on Fecal and Salivary Glucocorticoids and Immunoglobulin A in Female Captive Asian Elephants in Thailand

Kosaruk

Brown

Plangsangmas

et al. 2020

Animals

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Asian elephants have been an important part of wildlife ecotourism in Thailand for over two decades. Elephants in tourist camps are exposed to a variety of management styles and daily activities that can potentially affect health and welfare. This study investigated relationships between a novel welfare biomarker, immunoglobulin A (IgA), and daily camp activities, and compared results to glucocorticoid (GC) measures. Often no-riding camps are portrayed as providing better welfare than camps that offer riding. Therefore, we predicted that elephants at no-riding camps would have lower GC and higher IgA concentrations, and a low GC/IgA ratio. Forty-four female elephants from six elephant camps were divided into three groups based on riding activities: saddle-riding, bareback-riding, and no-riding. Fecal and salivary samples were collected monthly for 1 year along with evaluations of body condition, foot health, and wounding. Camp environment and management varied among camps, although the major difference was in riding activities. Concentrations of GCs and IgA varied among the working groups, but not always consistently between sample matrices. Overall fecal glucocorticoid metabolite concentrations were lowest in the saddle-riding group. Only in one bareback-riding camp did the elephants exhibit a potentially positive welfare response with a low GC/IgA ratio over time. Other results varied between the two biomarkers, with considerable variability across camps, suggesting there is more to good welfare than whether elephants participate in riding or not. Several other human-induced stressors, like chaining, ankus use, and limited social opportunities are likely to be impacting well-being and should be considered to ensure management practices meet physical and psychological welfare needs.

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“…IL‐10 and TGF‐ β 4 produced by activated macrophages and T cells are cytokines with immunoregulatory and anti‐inflammatory properties (Hammer et al., ; Yitbarek et al., ). Studies have demonstrated that probiotics can regulate the expression of IL‐10 and TGF‐ β 4 of cytokines (Chen et al., ; Karaffová et al., ). In this study, dietary probiotics increased the gene expression of IL‐10 and TGF‐ β 4 cytokines, suggesting that probiotics upregulated the anti‐inflammatory functions against Salmonella infection.…”

Section: Discussionmentioning

confidence: 99%

The effects of the supplementation of multi‐strain probiotics on intestinal microbiota, metabolites and inflammation of young SPF chickens challenged with Salmonella enterica subsp. enterica

Chang

Teng

Lee

et al. 2019

Animal Science Journal

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This study assessed the effect of probiotics on cecal microbiota, cecal short‐chain fatty acids (SCFAs), and the gene expression of cytokines in young specific‐pathogen‐free (SPF) chickens infected with S. enterica subsp. enterica. One‐day‐old SPF chickens (n = 105) were randomly assigned to one of the three treatment groups: control (Cont) group, Salmonella‐infected (Sal) group, and a Salmonella‐infected group treated with multi‐strain probiotics (ProSal group). All chickens except those in the Cont group were challenged orally with 1 × 108 cfu/ml of Salmonella 4 days after hatching. Chickens in the Sal group exhibited more abundance of Proteobacteria than those in the Cont and ProSal groups. At the genus level, chickens in ProSal group exhibited increased numbers of Lactobacillus and Oscillospira compared with those in the other groups. Chickens in the ProSal group exhibited a significant increase of cecal SCFAs compared with chickens in the Sal group. Chickens in the ProSal group exhibited increased gene expression of anti‐inflammatory cytokines, IL‐10 and TGF‐β4, and decreased expression of the proinflammatory cytokine, IFN‐γ, in the cecal tonsil compared with those in the Sal group. The results of this study indicated that the administration of probiotics can modulate microbiota, SCFAs, and immunomodulatory activity in SPF chickens.

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Interaction of TGF-β4 and IL-17 with IgA secretion in the intestine of chickens fed with E. faecium AL41 and challenged with S. Enteritidis

Cited by 29 publications

References 25 publications

Influence of oral application of Enterococcus faecium AL41 on TGF-β4 and IL-17 expression and immunocompetent cell distribution in chickens challenged with Campylobacter jejuni

Influence of oral application of Enterococcus faecium AL41 on TGF-β4 and IL-17 expression and immunocompetent cell distribution in chickens challenged with Campylobacter jejuni

Effect of Tourist Activities on Fecal and Salivary Glucocorticoids and Immunoglobulin A in Female Captive Asian Elephants in Thailand

The effects of the supplementation of multi‐strain probiotics on intestinal microbiota, metabolites and inflammation of young SPF chickens challenged with Salmonella enterica subsp. enterica

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