Interaction of Taurine With Metal Ions

O'Brien, Eimear C.; Farkas, Etelka; Nolan, Kevin B.

doi:10.1007/0-306-46838-7_39

Cited by 3 publications

(3 citation statements)

References 12 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is unclear why taurine enhanced copper-stimulated dopamine oxidation. A recent abstract (O'Brien et al, 1999) suggests that taurine may form a stable complex with copper. It is unknown if this complex merely enhances copper solubility or if it also enhances catalytic activity for oxidation reactions.…”

Section: Discussionmentioning

confidence: 98%

Taurine inhibition of metal-stimulated catecholamine oxidation

et al. 2000

View full text Add to dashboard Cite

Taurine is an abundant amino acid found in mammalian tissues and it has been suggested to have cytoprotective functions. The aim of the present study was to determine if taurine had the potential to reduce oxidative stress associated with metal-stimulated catecholamine oxidation. Taurine and structural analogs of taurine were tested for their ability to inhibit metal-stimulated quinone formation from dopamine or L-dopa. Oxidative damage to proteins and lipids were also assessed in vitro and the effects of taurine were determined. Taurine (20 mM) was found to decrease significantly ferric iron (50-500 microM)- and manganese (10 microM)-stimulated L-dopa or dopamine oxidation. Taurine had no effect on zinc-induced dopamine oxidation and slightly potentiated copper- and NaIO(4)-stimulated quinone formation. Ferric iron-stimulated lipid peroxidation was not affected by taurine (1-20 mM). Protein carbonyl formation induced by ferric iron (500 microM) and L-dopa (500 microM) was significantly reduced by 10 mM taurine. The cytotoxicity of L-dopa (250 microM) and ferric chloride (75 microM) to LLC-PK(1) cells was attenuated by 10 mM taurine or hypotaurine. Homotaurine alone stimulated L-dopa oxidation and potentiated the cytotoxic effects of ferric iron. Homotaurine was found to be cytotoxic when combined with L-dopa or L-dopa/iron. In contrast, hypotaurine inhibited quinone formation and protected LLC-PK(1) cells. These studies suggest that taurine may exhibit cytoprotective effects against the oxidation products of catecholamines by acting as a scavenger for free radicals and cytotoxic quinones.

show abstract

Section: Discussionmentioning

confidence: 98%

Taurine inhibition of metal-stimulated catecholamine oxidation

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Taurine can play an important role for antioxidant processes in cell cultivations . It is capable of building stable complexes with Cu 2+ and therefore might affect tryptophan fluorescence in a similar way as described for methionine . Pyridoxamine, pyridoxal, riboflavin, and 5‐hydroxtryptophan are fluorescent compounds and therefore correlate strongly with the 2D FL spectra.…”

Section: Resultsmentioning

confidence: 99%

Towards robust cell culture processes — Unraveling the impact of media preparation by spectroscopic online monitoring

Brunner

Brosig

Losing

et al. 2019

Engineering in Life Sciences

View full text Add to dashboard Cite

Biopharmaceutical manufacturing processes can be affected by variability in cell culture media, e.g. caused by raw material impurities. Although efforts have been made in industry and academia to characterize cell culture media and raw materials with advanced analytics, the process of industrial cell culture media preparation itself has not been reported so far. Within this publication, we first compare mid‐infrared and two‐dimensional fluorescence spectroscopy with respect to their suitability as online monitoring tools during cell culture media preparation, followed by a thorough assessment of the impact of preparation parameters on media quality. Through the application of spectroscopic methods, we can show that media variability and its corresponding root cause can be detected online during the preparation process. This methodology is a powerful tool to avoid batch failure and is a valuable technology for media troubleshooting activities. Moreover, in a design of experiments approach, including additional liquid chromatography–mass spectrometry analytics, it is shown that variable preparation parameters such as temperature, power input and preparation time can have a strong impact on the physico‐chemical composition of the media. The effect on cell culture process performance and product quality in subsequent fed‐batch processes was also investigated. The presented results reveal the need for online spectroscopic methods during the preparation process and show that media variability can already be introduced by variation in media preparation parameters, with a potential impact on scale‐up to a commercial manufacturing process.

show abstract

“…Biochemical and medicinal interest in taurine increased following Hayes et al research's on it in 1975 [1]. In order to maintain calcium homeostasis, it also functions as an antioxidant, osmoregulatory, neurotransmitter, growth regulator, cell membrane protector, a component of bile salt, and anticonvulsant and insulinogenic agent [2]. Taurine is a water-soluble colorless molecule that plays a significant function in the brain, liver, muscle, blood, cardiovascular, reproductive systems, and retina [3].…”

Section: Introductionmentioning

confidence: 99%

Preparation of voltammetric sensors for taurine detection from Cu(II)-loaded carbon materials

Emre

2023

Bull. Chem. Soc. Eth.

View full text Add to dashboard Cite

ABSTRACT. In this study, for the detection of taurine in philological fluids, modified electrodes were prepared from glassy carbon electrodes with MWCNT (multi-wall carbon nanotube) + Cu(NO3)2 + carbon-based material. A carbon-based material was obtained by carbonization of Pinus sylvestris branch needles (CPS). Cu(NO3)2 and MWCNT structure (Cu(NO3)2/MWCNT) were added to the carbon-based material and the obtained mixture was used in the modification of the glassy carbon electrode surface. The surface morphology and structure of the prepared electrodes were characterized by electron microscopy scanning (SEM) and atomic force microscopy (AFM). Taurine detection with a modified electrode was made by cyclic voltammetry (CV) in 0.1 M KCl (vs. Ag/AgCl). The calibration curve of the modified electrode for taurine in the 10-150 µM range showed a linear response, and R2 value was 0.976. Limit of detection (LOD) and limit of quantification (LOQ) of the modified electrode were found 19 µM and 9.75 µM, respectively. The application of the electrode as a chemical sensor was carried out with commercially available energy drink samples. Satisfactory results have been obtained from the samples. The sensor can be used for routine taurine analysis. This electrode can quickly determine the amount of taurine in real samples with 90% accuracy in two minutes. KEY WORDS: Taurine detection, Modified electrode, MWCNT, Sensor, Cyclic voltammetry Bull. Chem. Soc. Ethiop. 2023, 37(6), 1565-1576. DOI: https://dx.doi.org/10.4314/bcse.v37i6.20

show abstract

Interaction of Taurine With Metal Ions

Cited by 3 publications

References 12 publications

Taurine inhibition of metal-stimulated catecholamine oxidation

Taurine inhibition of metal-stimulated catecholamine oxidation

Towards robust cell culture processes — Unraveling the impact of media preparation by spectroscopic online monitoring

Preparation of voltammetric sensors for taurine detection from Cu(II)-loaded carbon materials

Contact Info

Product

Resources

About