Interaction of Sucrose, a Morphactin and 8-Hydroxyquinoline in the Regulation of Petal Senescence of Calendula officinalis L. Cut Flowers

Abstract

Cut flowers of Calendula officinalis L. as scapes were maintained in holding solutions of 0.1M sucrose, 20 M methyl chlorflurenol (a morphactin, MOR), 1.5 mM 8hydroxyquinoline (8-HQ), sucrose + MOR, sucrose + 8-HQ and sucrose + MOR + 8-HQ in the laboratory under continuous light. Untreated sets were also present where scapes getting the supply of double distilled water (DDW). Combined applications of sucrose + 8-HQ and sucrose + MOR + 8-HQ were very effective in minimizing the loss of fresh weight of scapes and moisture content. These combinations and also individual sucrose treatment as well as sucrose + MOR were very useful to reduce the loss in flower diameter by maintaining hydration and turgidity. Sucrose + MOR + 8-HQ combination was also unique in exhibiting maximum rise in protein amount on fresh weight basis between 0 and 3 and 3 and 6-day and on dry weight basis between 0 and 3-day. Lesser GPOX activity in petals of flower scapes subjected to 8-HQ, MOR and sucrose treatments may suggest lesser production of H2O2. Further, used chemicals such as sucrose, MOR and 8-HQ could reduce the lipid peroxidation process in petals and the best treatment was sucrose + MOR + 8-HQ followed by sucrose + 8-HQ > sucrose + MOR > MOR> 8-HQ > sucrose > DDW.

Comparative assessment of sucrose, 8-hydroxyquinoline and 5-sulfosalicylic acid in the regulation of petal senescence in cut scapes of Aster novae belgii L., Matricaria parthenium L. and Gaillardia pulchella Foug.

“…The method of extraction of protein has been described earlier (Singh et al, 2018). Protein content was estimated by the method of Bradford (1976) using Coomassie Brilliant blue G-250.…”