Interaction of single and multi wall carbon nanotubes with the biological systems: tau protein and PC12 cells as targets

Zeinabad, Hojjat Alizadeh; Zarrabian, Alireza; Saboury, Ali Akbar; Alizadeh, Ali Mohammad; Falahati, Mojtaba

doi:10.1038/srep26508

Cited by 124 publications

(93 citation statements)

References 59 publications

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“…Most cytotoxicity research in the literature has used the concentration range of carbon nanotubes between 0.1 and 200 μg mL −1 with maximum incubation up to 96 h on different types of normal cell lines [37–40]. This is because carbon nanotubes is generally associated with a concentration- and time-dependent increase in cell death as investigated by the use of the MTT assay.…”

Section: Resultsmentioning

confidence: 99%

In vitro drug release characteristic and cytotoxic activity of silibinin-loaded single walled carbon nanotubes functionalized with biocompatible polymers

Tan

Karthivashan

Gani

et al. 2016

Chemistry Central Journal

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In this paper, we demonstrate the preparation of silibinin-loaded carbon nanotubes (SWSB) with surface coating agents via non-covalent approach as an effective drug delivery system. The resulting surface-coated SWSB nanocomposites are extensively characterized by Fourier transform infrared (FTIR) and Raman spectroscopies, ultraviolet–visible (UV–Vis) spectrometry and field emission scanning electron microscopy (FESEM). The FTIR and Raman studies show that an additional layer is formed by these coating agents in the prepared nanocomposites during the coating treatment and these results are confirmed by FESEM. Drug loading and release profiles of the coated SWSB nanocomposites in phosphate buffered saline solution at pH 7.4 is evaluated by UV–Vis spectrometry. The in vitro results indicate that the surface-modified nanocomposites, with SB loading of 45 wt%, altered the initial burst and thus, resulted in a more prolonged and sustained release of SB. In addition, these nanocomposites exhibit a pseudo-second-order release kinetic which was driven by the ion exchange between the ionized SWSB and the anions in the release medium. The cytotoxicity effect of the resulting nanocomposites on normal mouse fibroblast cells is evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. It is observed that the surfactant and polymer coating improved the biocompatibility of the SWSB nanocomposites significantly, which deem further exploitation for their application as potential anticancer drug delivery system.

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Section: Resultsmentioning

confidence: 99%

In vitro drug release characteristic and cytotoxic activity of silibinin-loaded single walled carbon nanotubes functionalized with biocompatible polymers

Tan

Karthivashan

Gani

et al. 2016

Chemistry Central Journal

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“…SWCNT and MWCNT were synthesized according to the protocol developed previously at the Department of Biophysics of the University of Tehran and obtained as a donation . The diameter of the SWCNT was in the range of 1.3–2.3 nm and MWCNT was 5.5 nm.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial oxidative stress and dysfunction induced by single‐ and multiwall carbon nanotubes: A comparative study

Ghanbari

Nasarzadeh

Seydi

et al. 2017

J Biomedical Materials Res

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With the ever-increasing use of carbon nanotubes (CNTs) in health-related and engineering applications, the hazardous risks of this material have become a major concern. It is well known that CNTs accumulate with cytotoxic and genotoxic levels within vital organs. It has also been shown that treating cell cultures with CNTs resulted in cell-cycle arrest and increased apoptosis/necrosis. The goal of this pilot study is to perform a comprehensive comparative study on the toxicity of single-wall (SW) and multiwall (MW) CNTs in rat skin cells. Our results confirm a dose-dependent toxicity of SWCNTs and MWCNTs due to the loss of mitochondrial activity, increase in mitochondrial reactive oxygen species (ROS) formation, and mitochondrial membrane potential collapse before mitochondrial swelling. Moreover, disturbance in the oxidative phosphorylation is observed by a decrease in ATP level. These events induced the release of cytochrome c via outer membrane rupture or MPT pore opening and subsequently programmed cell death of all doses compared to control group. Our results demonstrate that although MWCNTs can be very toxic, SWCNTs cause more mitochondrial damage to the cells. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2047-2055, 2017.

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“…In neuronal cells, microtubule‐associated proteins, namely tau proteins, play a major role in MT polymerization and axon growth . In vitro interaction between tau proteins and NPs such as iron NPs and CNTs have been previously described in the literature. Based on a recent study in SH‐SY5Y neuroblastoma cells by Mao et al, the interaction between TiO 2 NPs and tau proteins resulted in a decrease in MT density, disorganization, and disruption of MT.…”

Section: Effects Of Intracellular Nanoparticles On Adhesion Cytoskelmentioning

confidence: 99%

Nanoparticle–Cell Interaction: A Cell Mechanics Perspective

et al. 2018

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Progress in the field of nanoparticles has enabled the rapid development of multiple products and technologies; however, some nanoparticles can pose both a threat to the environment and human health. To enable their safe implementation, a comprehensive knowledge of nanoparticles and their biological interactions is needed. In vitro and in vivo toxicity tests have been considered the gold standard to evaluate nanoparticle safety, but it is becoming necessary to understand the impact of nanosystems on cell mechanics. Here, the interaction between particles and cells, from the point of view of cell mechanics (i.e., bionanomechanics), is highlighted and put in perspective. Specifically, the ability of intracellular and extracellular nanoparticles to impair cell adhesion, cytoskeletal organization, stiffness, and migration are discussed. Furthermore, the development of cutting-edge, nanotechnology-driven tools based on the use of particles allowing the determination of cell mechanics is emphasized. These include traction force microscopy, colloidal probe atomic force microscopy, optical tweezers, magnetic manipulation, and particle tracking microrheology.

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Interaction of single and multi wall carbon nanotubes with the biological systems: tau protein and PC12 cells as targets

Cited by 124 publications

References 59 publications

In vitro drug release characteristic and cytotoxic activity of silibinin-loaded single walled carbon nanotubes functionalized with biocompatible polymers

In vitro drug release characteristic and cytotoxic activity of silibinin-loaded single walled carbon nanotubes functionalized with biocompatible polymers

Mitochondrial oxidative stress and dysfunction induced by single‐ and multiwall carbon nanotubes: A comparative study

Nanoparticle–Cell Interaction: A Cell Mechanics Perspective

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