“…2 A and B). Since the interaction of ligand molecules with HSA causes a partial decrease in the emission signal of Trp214 in albumin, it is reasonable to hypothesize that MI-1851 does not interact with the protein or they form only poorly stable complexes [17] , [18] . Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The potential interaction of inhibitor MI-1851 with HSA was examined employing fluorescence quenching studies. Increasing amounts of MI-1851 (final concentrations: 0–10 μM) were added to HSA (2 μM) in phosphate-buffered saline (PBS, pH 7.4), after which the emission spectra were collected using 295 nm excitation wavelength [17] , [18] . Absorption spectra of MI-1851 in PBS was also recorded for the correction of the potential inner-filter effect, which was performed as it has been reported [17] , [18] .…”
“…2 A and B). Since the interaction of ligand molecules with HSA causes a partial decrease in the emission signal of Trp214 in albumin, it is reasonable to hypothesize that MI-1851 does not interact with the protein or they form only poorly stable complexes [17] , [18] . Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The potential interaction of inhibitor MI-1851 with HSA was examined employing fluorescence quenching studies. Increasing amounts of MI-1851 (final concentrations: 0–10 μM) were added to HSA (2 μM) in phosphate-buffered saline (PBS, pH 7.4), after which the emission spectra were collected using 295 nm excitation wavelength [17] , [18] . Absorption spectra of MI-1851 in PBS was also recorded for the correction of the potential inner-filter effect, which was performed as it has been reported [17] , [18] .…”
“…53 In human plasma, avonolignans and their sulfated metabolites are mainly bound to a specic site of serum albumin. 63,64 Flavonolignans are metabolized predominantly via phase II reactions, i.e., conjugation (methylation, 65 sulfation, 66 glucuronidation, 67 and glutathione conjugation 68 64), (Fig. 11).…”
Section: Reviewmentioning
confidence: 99%
“…53 In human plasma, flavonolignans and their sulfated metabolites are mainly bound to a specific site of serum albumin. 63,64…”
Section: Pharmacokinetics Of Silymarin Flavonolignansmentioning
confidence: 99%
“…103,104 The sulfate conjugates of silybin A, silybin B, isosilybin A, silychristin, and 2,3-dehydrosilychristin were mostly less potent inhibitors of CYPs 2C9, 2C19, 2D6, and 3A4, but 2,3-dehydrosilychristin-19- O -sulfate showed the strongest inhibitory effect on CYP 3A4. 63…”
Section: Toxicity Adverse Effects and Interactions With Other Drugsmentioning
Recent developments in chemistry, biosynthesis, analytical methods, and transformations of flavonolignans from silymarin are presented. Their pharmacology, biological activities, SAR and safety with special attention to the chirality are discussed.
The effects of the flow rate, the injection volume, and the mobile phase ratio on the retention time and the resolution of separation of silybin were examined using Box-Behnken design on a symmetry prep C18 column. The optimum values of the operating parameters were 8 ml/min for the flow rate, 3 ml for the injection volume, and 40:60 for the mobile phase ratio. Under the optimal conditions, the retention time of silybin B was 59.552 min and the resolution of silybin A and silybin B was 1.0696, where the purities of the obtained silybin A and silybin B were both over 99% by ultra-performance liquid chromatography. The preparative-liquid chromatography simulation was applied with COMSOL. The optimization results of the response surface are consistent with the experiment and the simulation results. The structures of the compounds were identified by ultra-performance liquid chromatography-tandem mass spectrometry and ultra-performance liquid chromatography-time of flight mass spectrometry.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
