Interaction of silver atomic quantum clusters with living organisms: bactericidal effect of Ag₃clusters mediated by disruption of topoisomerase–DNA complexes

Abstract

Ag3 clusters distort DNA conformation altering the binding of doxorubicin and enzymes. As a novel aspect, the Ag3 bactericidal activity is described.

On the Stability of Cu5 Catalysts in Air Using Multireference Perturbation Theory

“…The synthesis of such quantum clusters made of just a few metal atoms has been achieved by kinetic control using an electrochemical technique without employing any surfactants or capping agents. 5 , 6 This technique has opened the possibility for numerous applications ranging from cancer-therapeutic drugs 5 , 7 to efficient hydrogen photoproduction. 8 …”

Silver Atomic Quantum Clusters of Three Atoms for Cancer Therapy: Targeting Chromatin Compaction to Increase the Therapeutic Index of Chemotherapy

“…This intercalation is strictly dependent on the number of silver atoms in the cluster, being exclusive of Ag 3 ‐AQCS and independent of the type of base pairs (AT of GC) of the double helix . Therefore, it is essential to obtain pure and highly concentrated Ag 3 ‐AQCs without surfactants or other strong binding ligands that could mask their biological activity, which is a substantial challenge. Additionally, the Ag 3 ‐AQC concentration required for this study is roughly two orders of magnitude higher than that of previous studies .…”

“…Therefore, it is essential to obtain pure and highly concentrated Ag 3 ‐AQCs without surfactants or other strong binding ligands that could mask their biological activity, which is a substantial challenge. Additionally, the Ag 3 ‐AQC concentration required for this study is roughly two orders of magnitude higher than that of previous studies . Therefore, using the kinetic control concept we developed a new and efficient electrochemical method.…”

“…The effect of Ag 3 ‐AQCs on DNA unwinding and subsequent lengthening of the double helix is very strong . As a result of the topological modification of the DNA, Ag 3 ‐AQCs inhibit the binding of DNA sequence‐specific binding proteins to DNA and the binding of DNA topology‐dependent binding proteins such as bacterial topoisomerase IV and DNA gyrase . Inhibitors of human topoisomerases are currently used as anticancer agents .…”

Interacción de ARN con doxorrubicina: Influencia del ligando y del metal en la actividad biológica de complejos organometálicos

“…Cisplatin (line 3) was used as a reference for covalent binding, showing the OC and SC bands migrates more than free pUC18. L4-Ru (lanes 4-6) and L4-Rh (lanes 7-9) cause dosedependant retardation in the migration of supercoiled DNA bands which can be related to intercalation processes [216]. When the amount of L4-Rh is increased, a vanishing of the band intensity takes place, pointing out the precipitation of large amount of Rhodium coordinated to DNA.…”