Interaction of root colonizing biocontrol agents demonstrates the antagonistic effect against Fusarium oxysporum f. sp. lycopersici on tomato

Patel, Stuti; Saraf, Meenu

doi:10.1007/s10658-017-1192-y

Cited by 16 publications

(4 citation statements)

References 22 publications

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“…Compared to control treatment, bioformulation-treated bananas contained lower chlorophyll content. Similar to other reports, BCA-treated plants contained lower or showed no significant difference in chlorophyll content than control treatment ( Patel & Saraf 2017a ; 2017b ). The overall improved plant growth in bioformulation-treated Berangan could have contributed to lower chlorophyll content as energy was diverted for vegetative growth under stress conditions ( Teh et al 2015 ).…”

Section: Resultssupporting

confidence: 90%

A Consortium of Pseudomonas aeruginosa and Trichoderma harzianum for Improving Growth and Induced Biochemical Changes in Fusarium Wilt Infected Bananas

Wong

Zulperi

Saidi

et al. 2021

tlsr

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Fusarium wilt of banana cannot be effectively controlled by current control strategies. The most virulent form that caused major losses in the banana production is Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc-TR4). Biocontrol of Foc-TR4 using microbial antagonists offers a sustainable and eco-friendly alternative. A consortium of biocontrol agents (BCAs), Pseudomonas aeruginosa DRB1 and Trichoderma harzianum CBF2 was formulated into pesta granules, talc powder, alginate beads and liquid bioformulations. Previous study indicated bioformulations containing both BCAs successfully reduced the disease severity of Foc-TR4. To date, the biocontrol mechanism and plant growth promoting (PGP) traits of a consortium of BCAs on infected bananas have not been explored. Therefore, the study was undertaken to investigate the effect of a consortium of DRB1 and CBF2 in the growth and biochemical changes of Foc-TR4 infected bananas. Results indicated pesta granules formulation produced bananas with higher biomass (fresh weight – 388.67 g), taller plants (80.95 cm) and larger leaves (length – 39.40 cm, width – 17.70 cm) than other bioformulations. Applying bioformulations generally produced plants with higher chlorophyll (392.59 μg/g FW–699.88 μg/g FW) and carotenoid contents (81.30 μg/g FW–120.01 μg/g FW) compared to pathogen treatment (chlorophyll – 325.96 μg/g FW, carotenoid – 71.98 μg/g FW) which indicated improved vegetative growth. Bioformulation-treated plants showed higher phenolic (49.58–93.85 μg/g FW) and proline contents (54.63 μg/g FW–89.61 μg/g FW) than Foc-TR4 treatment (phenolic – 46.45 μg/g FW, proline – 28.65 μg/g FW). The malondialdehylde (MDA) content was lower in bioformulation treatments (0.49 Nm/g FW–1.19 Nm/g FW) than Foc-TR4 treatment (3.66 Nm/g FW). The biochemical changes revealed that applying bioformulations has induced host defense response by increasing phenolic and proline contents which reduced root damage caused by Foc-TR4 resulting in lower MDA content. In conclusion, applying bioformulations containing microbial consortium is a promising method to improve growth and induce significant biochemical changes in bananas leading to the suppression of Foc-TR4.

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Section: Resultssupporting

confidence: 90%

A Consortium of Pseudomonas aeruginosa and Trichoderma harzianum for Improving Growth and Induced Biochemical Changes in Fusarium Wilt Infected Bananas

Wong

Zulperi

Saidi

et al. 2021

tlsr

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“…The ability of members of the B. subtilis -like group to biosynthesize specialized compounds with antimicrobial activity have made this species attractive for use as biocontrol agents ( Caulier et al., 2019 ; Penha et al., 2020 ). Thus, treatment of tomato seeds with antifungal B. velezensis AP3 ( Medeiros and Bettiol, 2021 ), B. subtilis MMS9 ( Patel and Saraf, 2017 ), and B. amyloliquefaciens FZB24 ( Elanchezhiyan et al., 2018 ) suppressed Fusarium wilt of the plant. In our study, the antifungal DD6, which contains biosynthetic gene clusters (BGC) associated with well-known antimicrobial secondary compounds, was unable to significantly decrease the development of symptoms of Fusarium wilt disease in tomato ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

Bacillus cereus EC9 protects tomato against Fusarium wilt through JA/ET-activated immunity

2022

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The mechanisms of action and the limitations of effectiveness of natural biocontrol agents should be determined in order to convert them into end products that can be used in practice. Rhizosphere Bacillus spp. protect plants from various pathogens by displaying several modes of action. However, the ability of Bacillus spp. to control plant diseases depends on the interaction between the bacteria, host, and pathogen, and the environmental conditions. We found that soil drenching of tomato plants with the non-antifungal Bacillus cereus strain EC9 (EC9) enhances plant defense against Fusarium oxysporum f. sp. lycopersici (Fol). To study the involvement of plant defense-related phytohormones in the regulation of EC9-activated protection against Fol, we conducted plant bioassays in tomato genotypes impaired in salicylic acid (SA) accumulation, jasmonic acid (JA) biosynthesis, and ethylene (ET) production, and analyzed the transcript levels of pathways-related marker genes. Our results indicate that JA/ET-dependent signaling is required for EC9-mediated protection against Fol in tomato. We provide evidence that EC9 primes tomato plants for enhanced expression of proteinase inhibitor I (PI-I) and ethylene receptor4 (ETR4). Moreover, we demonstrated that EC9 induces callose deposition in tomato roots. Understanding the involvement of defense-related phytohormones in EC9-mediated defense against Fusarium wilt has increased our knowledge of interactions between non-antifungal plant defense-inducing rhizobacteria and plants.

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“…The interaction between B. simplex on F. camptoceras revealed that the Bacillus caused a complete mycoparasitism on the fungal growth. The bacterial growth was seen colonizing the hyphae and adhering, thus leading to maceration of hyphal tissues or antagonistic effect may be due to direct effect of antagonistic bacteria against F. camptoceras through cloning their hyphae to prevent their continued growth (Patel and Saraf 2017).…”

Section: Effect Of B Simplex On Volatile Oils and Fixed Oilsmentioning

confidence: 99%

Potential activities of Bacillus simplex as a biocontrol agent against root rot of Nigella sativa caused by Fusarium camptoceras

Al-Sman

Abo-Elyousr

Eraky

et al. 2019

Egypt J Biol Pest Control

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This study aimed to investigate the impact of two isolates of Bacillus simplex (PHYB1 and PHYB9) for controlling the root rot disease of black cumin (Nigella sativa) caused by Fusarium camptoceras (PHYF1) under greenhouse and field conditions at Assiut Governorate, Egypt. The highest reduction percentage of infection (22.5%) was recorded by the treatment of PHYB1 as suspension than the control (60%). Both isolates of Bacillus spp. provided the root and foliar dry weight under greenhouse and seed production in the field. The results showed that the isolate PHYB1 as a formulation gave the highest impact in the root dry weight (0.28 g/plant), followed by PHYB9 (0.22 g/plant), with insignificant difference between them. PHYB9 suspension also provided the highest seed production (27.97 g/plant), whereas PHYB9 as a formulation gave the lowest (24.08 g/plant). Studies on the interaction between Bacillus spp. on F. camptoceras by scanning electron microscope (SEM) revealed that both caused a complete mycoparasitism on the fungal growth. The bacterial growth was seen to adhere and colonize the hyphae, resulting in hyphal tissue maceration. Therefore, the use of both isolates of Bacillus spp. to control root rot disease of black cumin under greenhouse and field conditions can be recommended.

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Interaction of root colonizing biocontrol agents demonstrates the antagonistic effect against Fusarium oxysporum f. sp. lycopersici on tomato

Cited by 16 publications

References 22 publications

A Consortium of Pseudomonas aeruginosa and Trichoderma harzianum for Improving Growth and Induced Biochemical Changes in Fusarium Wilt Infected Bananas

A Consortium of Pseudomonas aeruginosa and Trichoderma harzianum for Improving Growth and Induced Biochemical Changes in Fusarium Wilt Infected Bananas

Bacillus cereus EC9 protects tomato against Fusarium wilt through JA/ET-activated immunity

Potential activities of Bacillus simplex as a biocontrol agent against root rot of Nigella sativa caused by Fusarium camptoceras

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