Interaction of RNA polymerase mutations in haploid and merodiploid cells of Escherichia coli K-12

Ilyina, T. S.; Ovadis, Marianna; Mindlin, S. Z.; Gorlenko, Zh. M.; Khesin, R. B.

doi:10.1007/bf00332643

Cited by 41 publications

(7 citation statements)

References 23 publications

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“…According to this author, in rif-sf rif-r merodi ploid strains there is no evidence of two populations of RNA polymerases, and he interprets his results by hypothesizing some kind of intramolecular complementation between sensitive and resistant f3 subunits. Other authors claim that the rif-r mutation is, as a rule, recessive to the rif-s and propose an explanation that is in conflict with the one given by Babinet (105,106,181). All these problems require further consideration since, in some cases, the techniques employed have not always been sensitive enough to detect possible cases of different levels of intermediate dominance.…”

Section: Mutation To Rifampicin Resistancementioning

confidence: 98%

“…Temperature-sensitive mutants have. also been found but, although they also map near the arg H locus, it is not completely clear whether they are in the same cistron (49,84,99,100,181), A tempera ture-sensitive mutation has been isolated that seems to be located in a subunit Annu. Rev.…”

Section: Mutation To Rifampicin Resistancementioning

confidence: 99%

“…In E. coli merodiploids, the question of the dominance of the rif-r muta tion has been investigated. Cases both of dominance and of recessiveness have been reported (48,49,99,105,106,179,181).…”

Section: Mutation To Rifampicin Resistancementioning

confidence: 99%

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Rifamycins: A General View

Riva¹,

Silvestri²

1972

Annu. Rev. Microbiol.

126

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Section: Mutation To Rifampicin Resistancementioning

confidence: 98%

Section: Mutation To Rifampicin Resistancementioning

confidence: 99%

See 1 more Smart Citation

Rifamycins: A General View

Riva¹,

Silvestri²

1972

Annu. Rev. Microbiol.

126

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“…For example, bacteriophage T2, like T5, requires functional host RNA polymerase for normal development. When T2 growth was measured on rpoB/rpoB + merodiploids in the presence of rifampin, intermediate resistance of phage development to the antibiotic was observed (10). Still, although cmr mutations are completely recessive, one cannot exclude the possibility that their recessive nature is the result of subunit mixing.…”

Section: Isolation Of Permissive Colib+ Mutantsmentioning

confidence: 99%

Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid

Hull¹,

Moody²

1976

J Bacteriol

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A mutant derivative of Escherichia coli K-12 has been isolated which is permissive for bacteriophage T5 infection even when harboring a wild-type ColIb plasmid. The fully permissive phenotype was the result of two mutations that are located near the rpsL-rpsE region on the E. coli chromosome and are recessive to the wild-type alleles. These mutations had little or no effect on induction of colicin synthesis and did not affect the expression of antibiotic resistance by the resistance plasmids R64drd11 or R1drd19. Cells harboring the mutant alleles grew more slowly than isogenic wild-type derivatives in either minimal or complete media.

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“…If this finding is confirmed in in vitro studies, the system of isolating drug-resistant mutants from temperature-sensitive mutants in related pathways will provide a means for expanding catalogues of drug-resistant mutants. Interaction between rifr mutations and TS RNA polymerase mutations has been previously mentioned (4,5,8); in the case of one Ts-290 rift double mutant, the interaction has been VOL. 110, 1972 (7); DB5 = rift RNA polymerase mutant from DB3 and is probably a , mutation (6).…”

mentioning

confidence: 95%

New Rifampin-Resistant Mutant of Escherichia coli

Marshall

Gillespie

1972

J Bacteriol

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A rifampin-resistant ribonucleic acid (RNA) polymerase mutant, rift5l, derived from a presumptive RNA synthesis mutant of Escherichia coli K-12, complements rifr RNA polymerase mutants isolated from other strains of E. coli K-12.A temperature-sensitive (TS) mutant of Escherichia coli K-12, T8-290, is probably defective for ribonucleic acid (RNA) synthesis (8). It has no known mutations other than the one leading to temperature sensitivity. A relation between the 1 mutation and rifampin resistance (rifr) genes of E. coli has established; namely, when a rifr mutation isolated from a temperature-independent (Ti) strain is transferred to 78-290, each mutation suppresses the other (8). This suppression is observed with both the classical RNA polymerase (9) and the so-called "permeability" ritr muta tions (8), although the phenotypes are different in the two cases. T7rifr (polymerase) double mutants are as weakly ritr and almost as TS as T'-290, whereas Trifr ("permeability") double mutants are more rifr and are T1 (S. Marshall, unpublished data).Since the usual ritr mutations appear not to be fully expressed in 7'-290, the possibility arose that fully rift strains, isolated by mutation from P-290, might differ from those isolated from other K-12 strains. In this paper rift mutants that are not permeability mutants, as defined by loss of resistance of ethylenediaminetetraacetic acid-treated cells (8), are assumed to be mutants with altered RNA polymerase. Mutations in new cistrons can be detected by genetic complementation tests; partial diploids carrying ritr mutations in different cistrons should exhibit a rifampin-sensitive (rif) phenotype while those carrying rift mutations in the same gene show a ritr pheno-'Present addreas: Gerontology Research Center, Baltimore City Hospitals, Baltimore, Md. 21224. type. Indeed, rift ("permeability")/rift (polymerase) heterogenotes are phenotypically rifp

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Interaction of RNA polymerase mutations in haploid and merodiploid cells of Escherichia coli K-12

Cited by 41 publications

References 23 publications

Rifamycins: A General View

Rifamycins: A General View

Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid

New Rifampin-Resistant Mutant of Escherichia coli

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