Interaction of purified ergovaline from endophyte-infected tall fescue with synaptosomal ATPase enzyme system

Moubarak, Ali S.; Piper, Ed L.; West, Charles P.; Johnson, Z. B.

doi:10.1021/jf00027a012

Cited by 31 publications

(23 citation statements)

References 10 publications

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“…Twenty l of each supernatant from the enzyme assays were examined for the disappearance of ergotamine and its isomer by using a modification of the HPLC method described by Moubarak et al (11). A 20 l sample loop was fitted to a Gilson 714 HPLC system with two pumps, an auto-sample injector and a gradient programmer.…”

Section: Methodsmentioning

confidence: 99%

Hepatic Metabolism of Ergot Alkaloids in Beef Cattle by Cytochrome P450

Moubarak

Rosenkrans

2000

Biochemical and Biophysical Research Communications

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Section: Methodsmentioning

confidence: 99%

Hepatic Metabolism of Ergot Alkaloids in Beef Cattle by Cytochrome P450

Moubarak

Rosenkrans

2000

Biochemical and Biophysical Research Communications

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“…In sheep, increased daylight length that results in increased serum prolactin, which is importantly related to a spring moult and production of a summer coat (Lincoln 1990). As a specific cellular mechanism upon neural function, ergovaline inhibited Na+/K+ ATPase activity in a dose dependent manner in rat brain homogenates (Moubarak et al 1993).…”

Section: Animal Toxicosis-tall Fescuementioning

confidence: 99%

Anti-quality factors associated with alkaloids in eastern temperate pasture

Thompson¹,

Stuedemann²,

Hill³

2001

REM

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The greatest anti-quality associated with eastern temperature pasture grasses is the result of ergot alkaloids found in endophyte-infected (Neotyphodium ceonophialum) tall fescue (Festuca arundinacea Schreb.) The relationship between the grass and the endophyte is mutalistic with greater persistence and herbage mass as a result of the endophyte. Ergot alkaloids reduce growth rate, lactation, and reproduction in livestock. Significant effects are the result of elevated body temperature and reduced peripheral blood flow such that necrosis may result. Perturbations also occur in a variety of body systems. Planting new pastures with seed containing a "non-toxic" endophyte appears to be a potential solution. Ergotism results from the ingestion of the scelerotia of Claviceps purpurea containing ergot alkaloids found on seed heads. Ergotism resembles the effects of endophyte-infected tall fescue. Endophyte-infected perennial ryegrass (Lolium perenne L.) contains ergot and lotirem alkaloids that result in reduced growth and tremors. Reed canarygrass (Phalaris Anundinacba L.) contains tryptamine, hordenine and gramine alkaloids that reduce growth. Annual ryegrass (Lolium multiplorum L. may contain galls with cornetoxins which result in neurological signs.

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“…The concentrations of ergovaline in the stems and leaves of tall fescue usually range between 200 and 600 ppb and in the seed heads from 2000 to 4000 ppb (Evans et al, 2004b). Estimates of the minimum threshold toxic concentrations of ergovaline in fescue, which have the potential to cause clinical problems in livestock, range from 50 to 500 ppb (Moubarak et al, 1993;Tor-Agbidye et al, 2001;AldrichMarkham et al, 2003;Evans et al, 2004b). These minimal threshold concentrations depend on the species affected, the type of syndrome being considered, and environmental temperatures, and they will most likely vary with geographical location.…”

Section: General Classification and Toxicitymentioning

confidence: 99%

“…In addition to the aforementioned decrease in serum cholesterol and triglycerides, there are also decreases in alkaline phosphatase, γ-glutamyltransferase, aspartate aminotransferase, alanine aminotransferase, creatinine kinase, lipase, and lactic dehydrogenase (Oliver, 1997). Potentially, the inhibition of ATPase in brain and kidney may be mediated by the same mechanism (Moubarak et al, 1993).…”

Section: Serum Enzyme Decreasementioning

confidence: 99%