Interaction of Proteins with Dextransulfate in Aqueous Medium Part 2. Nonequilibrium Phenomena

Gurov, A. N.; Wajnerman, E. S.; Tolstoguzov, V. B.

doi:10.1002/star.19770290603

Cited by 20 publications

(15 citation statements)

References 10 publications

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“…This protective action can also increase the stability of protein suspensions and oil-in-water emulsions stabilized by soluble protein±anionic polysaccharide complexes. 5,6,27 Thus, the hydrophilic-hydrophobic character of a complex particle is controlled by the junction zone/polysaccharide chain ratio. An increase in junction zone content can decrease the solubility (dispersability) and improve the surface activity of functional protein complexes.…”

Section: Solubility and Stabilitymentioning

confidence: 99%

“…The composition and properties of protein-multivalent cation-anionic polysaccharide complexes depend on their preparation conditions. 17,27 These triple complexes are usually of non-equilibrium nature in model and real food systems. 5,6,17,18,27 For instance, the consistency of emulsions stabilized by triple complexes can be varied from a liquid to a thixotropic solid state.…”

Section: Solubility and Stabilitymentioning

confidence: 99%

“…17,27 These triple complexes are usually of non-equilibrium nature in model and real food systems. 5,6,17,18,27 For instance, the consistency of emulsions stabilized by triple complexes can be varied from a liquid to a thixotropic solid state. This depends on the addition of multivalent cations (such as Ca and Cu-ions) added to a mixed solution of a protein and an anionic polysaccharide before and after oil emulsification.…”

Section: Solubility and Stabilitymentioning

confidence: 99%

“…Protein molecules can cross-link polysaccharide chains and form a three-dimensional network of precipitated complex gel particles. 5,6,27 The system of the same composition can be prepared by mixing the same biopolymer solutions at alkaline pH (where a stable mixed solution, which does not contain electrostatic complexes is formed) and by lowering of the pH from alkaline pH to the same acidic conditions. This second method of titration leads to dispersible interbiopolymer complexes.…”

Section: Macromolecular Ingredient Interactions 721 Basic Principlesmentioning

confidence: 99%

“…It has been studied in detail for mixtures of bovine serum albumin and dextran sulfate. 5,6,26,27 For instance, bovine serum albumin±dextran sulphate complexes can be prepared by two different`mixing' and`titration' methods. These methods lead to the two different types of`mixing' (M) and`titration' (T) complexes, respectively.…”

Section: Macromolecular Ingredient Interactions 721 Basic Principlesmentioning

confidence: 99%

See 4 more Smart Citations

Ingredient interactions in complex foods: aggregation and phase separation

Tolstoguzov¹

2007

Understanding and Controlling the Microstructure of Complex Foods

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Section: Solubility and Stabilitymentioning

confidence: 99%

Section: Solubility and Stabilitymentioning

confidence: 99%

Section: Solubility and Stabilitymentioning

confidence: 99%

Section: Macromolecular Ingredient Interactions 721 Basic Principlesmentioning

confidence: 99%

Section: Macromolecular Ingredient Interactions 721 Basic Principlesmentioning

confidence: 99%

See 3 more Smart Citations

Ingredient interactions in complex foods: aggregation and phase separation

Tolstoguzov¹

2007

Understanding and Controlling the Microstructure of Complex Foods

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Glucosylceramide Mimics: Highly Potent GCase Inhibitors and Selective Pharmacological Chaperones for Mutations Associated with Types 1 and 2 Gaucher Disease

et al. 2013

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A series of iminoxylitol derivatives carrying a C-linked di-O-acyl or di-O-alkyl glyceryl substituent were prepared and characterized. All of these compounds, which were designed as glucosylceramide (GlcCer) mimics, were nanomolar inhibitors of lysosomal β-glucosidase (glucocerebrosidase, GCase). Two of these pseudoglycolipids were further evaluated for their ability to enhance the activity of mutant GCase in human Gaucher cells. Although the di-O-hexyl ether was surprisingly devoid of chaperoning activity on both N370S and L444P GCases, the di-O-decanoyl ester was a potent chaperone of the L444P hydrolase, capable of increasing the residual activity of the enzyme by a factor of two at a very low concentration (50 nM); such a significant effect on the L444P mutation in human fibroblasts has not yet been observed. In heat-stress studies, the diether was found to be much more effective in stabilizing the wild-type enzyme than the diester. Four representative pseudoglycolipids were also assayed as inhibitors of GlcCer synthase, because such compounds could find use in the substrate reduction therapy approach to treat lysosomal storage diseases, but these compounds revealed only moderate activity. As efficient pharmacological chaperones, new structures such as the di-C10 -ester constitute leads for the development of therapeutic agents for types 2 and 3 Gaucher disease, the most severe neuronopathic forms of this lysosomal disease.

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Successive multiple ionic polymer layer coated capillaries in the separation of proteins – Recombinant allergen variants as a case study

Weinbauer

Stutz

2010

Electrophoresis

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A successive multiple ionic polymer layer (SMIL) coating consisting of two pairs of poly(diallyldimethylammonium chloride) and dextran sulfate (DS) layers was applied for the separation of recombinant products of the major birch pollen allergen Betula verrucosa (Bet v 1a). The combination with volatile ammonium bicarbonate buffer at pH 6.70 offers the possibility for future MS hyphenation. The negative net charge of allergens required DS as terminal SMIL layer. The EOF was accelerated from 3.17x10(-8) m(2) V(-1) s(-1) in uncoated to 4.52x10(-8) m(2) V(-1) s(-1) in SMIL capillaries. Fresh prepared SMIL capillaries showed slight EOF acceleration due to gradual re-organization of SMIL structure until stabilization was achieved. Dry storage of SMIL capillaries prevented fluctuations in EOF and migration times and improved coating durability. However, the gradual reconstitution of entangled SMIL layers affected efficiency, but was cured by a 10 mmol/L NaOH rinsing step. Durability of SMIL capillaries in MS-applicable dimension was confirmed for > 70 runs and in total 42 h of voltage application with average intra-day precision of 0.22 and 0.79% and inter-day-precision of 0.91 and 1.17% for migration times of EOF and Bet v 1a, respectively. Final SMIL coating allowed for the separation of Bet v 1a, a hypoallergenic isoform and carbamylated variants with 150,000-685,000 plates.

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Interaction of Proteins with Dextransulfate in Aqueous Medium Part 2. Nonequilibrium Phenomena

Cited by 20 publications

References 10 publications

Ingredient interactions in complex foods: aggregation and phase separation

Ingredient interactions in complex foods: aggregation and phase separation

Glucosylceramide Mimics: Highly Potent GCase Inhibitors and Selective Pharmacological Chaperones for Mutations Associated with Types 1 and 2 Gaucher Disease

Successive multiple ionic polymer layer coated capillaries in the separation of proteins – Recombinant allergen variants as a case study

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