Interaction of Polyelectrolytes and Their Composites with Living Cells

Chanana, Munish; Gliozzi, Alessandra; Diaspro, Alberto; Chodnevskaja, Irina; Huewel, Sabine; Moskalenko, Vasiliy; Ulrichs, K.; Galla, Hans Joachim; Krol, Silke

doi:10.1021/nl0521219

Cited by 114 publications

(110 citation statements)

References 32 publications

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“…15 However, chitosan cannot dissolve in neutral buffer, and the use of PE films as a cell adhesive material is limited due to the cytotoxicity of polycations. 16,17 The appropriate choice of natural ECM components for preparation of the nanofilms is important to avoid cytotoxicity, and the typical ECM presents cell-adhesive moieties such as RGD (arginineglycineaspartic acid) and other functional moieties. 18 We selected FN and G to prepare nano-ECM films on the cell surface.…”

Section: Hierarchical Cell Manipulation Techniquementioning

confidence: 99%

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Matsusaki

2012

Bulletin of the Chemical Society of Japan

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The creation of artificial three-dimensional (3D) tissues possessing structure and function similar to natural tissue is a key challenge for implantable tissues in tissue engineering, and for model tissues in pharmaceutical assays. This account is a summary of our current research toward this challenge. We have developed a simple and unique bottom-up approach, hierarchical cell manipulation technique, using nanometer-sized layer-by-layer films consisting of fibronectin and gelatin (FNG) as a nano-extracellular matrix (nano-ECM). The FNG nanofilms were prepared directly on the cell surface, and we discovered that at least 6 nm thick FNG films acted as a stable adhesive surface for adhesion of the second cell layer. Various 3D-layered constructs consisting of single or multiple types of cells were successfully fabricated, and the higher cellular activities induced from the 3D-structures as compared to monolayer structure were observed. Furthermore, the multilayered constructs like a blood vessel wall structure indicated almost the same drug response as in vivo natural blood vessels, suggesting the possibility to use as an in vitro blood vessel model to analyze drug response. Recently, we also developed a rapid bottom-up approach by a single cell coating using FNG nanofilms, because the fabrication of twolayers (2L) is limited through the above technique due to the time required for stable cell adhesion. This rapid approach easily provided approximately eight-layered (8L) 3D-tissues after only one day of incubation. The layer number, cell type, and location were all successfully controlled by altering the seeding cell number and order. Moreover, fully and homogeneously vascularized tissues of 1 cm width and 50¯m height were obtained by a sandwich culture of the endothelial cells. These hierarchical cell manipulations will be promising to achieve one of the dreams of biomedical field, in vitro creation of artificial 3D-tissue models.

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Section: Hierarchical Cell Manipulation Techniquementioning

confidence: 99%

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Matsusaki

2012

Bulletin of the Chemical Society of Japan

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“…When the polymers are charged (polyelectrolytes), those with a charge opposite to that on the particle surface will adsorb onto it in a relatively fl at orientation and may neutralize or overcompensate the surface charge of the particle. Subsequent adsorption of oppositely charged polyelectrolytes on nanoparticles, leads to stable core-shell systems [85,86] . This method is known as the LbL (Layerby-Layer) technique [87] , and is widely applied not only to stabilize gold nanoparticle dispersions, but also to confer them with a desired surface charge, which is determined by the charge of the outmost polyelectrolyte layer [85].…”

Section: Physically Adsorbed Polymersmentioning

confidence: 99%

“…Subsequent adsorption of oppositely charged polyelectrolytes on nanoparticles, leads to stable core-shell systems [85,86] . This method is known as the LbL (Layerby-Layer) technique [87] , and is widely applied not only to stabilize gold nanoparticle dispersions, but also to confer them with a desired surface charge, which is determined by the charge of the outmost polyelectrolyte layer [85]. Besides synthetic charged polymers, also bio-polymers [88] such as DNA [89 -93] , dextran [94] , cellulose [95,96] , chitosan [97,98] and various peptides and proteins can adsorb onto the nanoparticle surface, and have been widely used to make gold nanoparticles suitable for bio-applications.…”

Section: Physically Adsorbed Polymersmentioning

confidence: 99%

Coating matters: the influence of coating materials on the optical properties of gold nanoparticles

Chanana¹,

Liz‐Marzán²

2012

Nanophotonics

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An essential element in the synthesis of nanomaterials based on gold nanoparticles comprises the control over parameters such as size, shape and composition, due to their strong infl uence on the properties of the particles. However, it is the coating material which often plays the primary role in tuning the size, morphology, and even plasmon resonance wavelength or mode multiplicity, as well as colloidal stability and functional versatility, ultimately determining the physical, chemical, optical, electronic and catalytic properties of the nanoparticles. Therefore, it is utterly important to select the adequate wet chemistry synthetic approach with the most suitable coating material for the preparation of gold nanoparticles with the desired requirements. Within this context, this review is focused on describing various types of organic and inorganic coating materials for gold nanoparticles that may notably affect their optical properties by either directly infl uencing the synthesis procedure or by changing their chemical and physical properties upon post-synthetic modifi cations, such that they exhibit novel and useful optical properties.

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“…Furthermore, our results show that PAA has negligible toxicity even when its concentration is high, up to 4 mg/mL ( Figure S1, Supporting Information). This avoids the undesired corrosive problems of basic/acidic buffers [9,10] or common polyelectrolyte toxicity [12,20,24] for modifying SC, and make PAA an ideal polymer to tune the NP SC while keeping the size and shape of the NPs unchanged. This approach allows a systematic and effective study of the role of SC on NP properties.…”

mentioning

confidence: 99%

Tuning the Properties of ZnO, Hematite, and Ag Nanoparticles by Adjusting the Surface Charge

Zhang¹,

Dong²,

Thurber³

et al. 2012

Advanced Materials

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A poly (acryl acid) (PAA) post-treatment method is performed to modify the surface charge of ZnO nanospheres, hematite nanocubes, and Ag nanoprisms from highly positive to very negative by adjusting the PAA concentration, to and greatly modify their photoluminescence, cytotoxicity, magnetism, and surface plasmon resonance. This method provides a general way to tune the nanoparticle properties for broad physicochemical and biological applications.

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Interaction of Polyelectrolytes and Their Composites with Living Cells

Cited by 114 publications

References 32 publications

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Development of Three-Dimensional Tissue Models Based on Hierarchical Cell Manipulation Using Nanofilms

Coating matters: the influence of coating materials on the optical properties of gold nanoparticles

Tuning the Properties of ZnO, Hematite, and Ag Nanoparticles by Adjusting the Surface Charge

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