Interaction of poly(ethylene glycol)-conjugated phospholipids with supported lipid membranes and their influence on protein adsorption

Yamamoto, Toshihiro; Teramura, Yuji; Itagaki, Toru; Arima, Yusuke; Iwata, Hiroo

doi:10.1080/14686996.2016.1240006

Cited by 39 publications

(43 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…About the complex surface, of which the zeta potential varied from negative to positive, followed AuNR functionalization, and then changed to negative after FA functionalization. Negatively charged nanocarriers have been demonstrated to have better tumor penetration . We diluted FA to different concentrations to obtain its standard concentration curve by UV–vis.…”

Section: Resultsmentioning

confidence: 99%

“…In this work, we designed the first strategy based on combining AuNRs with exosomes for tumor‐targeted chemo‐photothermal therapy. Previous studies have reported that the amphiphilic molecule 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine‐poly(ethylene glycol) (DSPE‐PEG) could assemble itself into the phospholipid layer of cells . In this strategy ( Scheme ), we generated exosomes by culturing donor cells in medium containing arginyl–glycyl–aspartic acid (RGD)‐functionalized DSPE‐PEG (DSPE‐PEG‐RGD) and sulfhydryl‐functionalized DSPE‐PEG (DSPE‐PEG‐SH) as our previous studies .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Designer Exosomes for Active Targeted Chemo‐Photothermal Synergistic Tumor Therapy

Wang

Dong

et al. 2018

Adv Funct Materials

134

111

View full text Add to dashboard Cite

Exosomes, naturally derived nanovesicles secreted from various cell types, can serve as an effective platform for the delivery of various cargoes, because of their intrinsic ability such as long blood circulation and immune escapinge. However, unlike conventional synthetic nanoparticles, drug release from exosomes at defined targets is not controllable. Moreover, endowing exosomes with satisfactory cancer-targeting ability is highly challenging. Here, for the first time, a biological and synthetic hybrid designer exosome is described with photoresponsive functionalities based on a donor cell-assisted membrane modification strategy. Practically, the designer exosome effectively accumulates at target tumor sites via dual ligand-mediated endocytosis. Then the localized hyperthermia induced by the conjunct gold nanorods under near-infrared irradiation impacts the permeability of exosome membrane to enhance drug release from exosomes, thus inhibiting tumor relapse in a programmable manner. The designer exosome combines the merits of both synthetic materials and the natural nanovesicles. It not only preserves the intrinsic functionalities of native exosome, but also gains multiple abilities for efficient tumor targeting, controlled release, and thermal therapy like synthetic nanocarriers. The versatile designer exosome can provide functional platforms by engineering with more multifarious functionalities from synthetic materials to achieve individualized precise cancer therapy in the future.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Designer Exosomes for Active Targeted Chemo‐Photothermal Synergistic Tumor Therapy

Wang

Dong

et al. 2018

Adv Funct Materials

134

111

View full text Add to dashboard Cite

show abstract

“…An alternative strategy is the hydrophobic insertion used to functionalize the EVs' membranes by exploiting the phospholipid composition of plasma membranes. Amphiphilic molecule DSPE-PEG, FDA approved for medical applications, can self-assemble in the phospholipid bilayer [144]. Based on this consideration, if DSPE-PEG is bound to the molecule of interest, it can be incorporated inside the cell membrane, making it overexpresses the molecule on its surface and producing EVs with the desired functionalization.…”

Section: Indirect Surface Functionalizationmentioning

confidence: 99%

Engineered Extracellular Vesicles as a Reliable Tool in Cancer Nanomedicine

Susa

Limongi

Dumontel

et al. 2019

Cancers

View full text Add to dashboard Cite

Fast diagnosis and more efficient therapies for cancer surely represent one of the huge tasks for the worldwide researchers’ and clinicians’ community. In the last two decades, our understanding of the biology and molecular pathology of cancer mechanisms, coupled with the continuous development of the material science and technological compounds, have successfully improved nanomedicine applications in oncology. This review argues on nanomedicine application of engineered extracellular vesicles (EVs) in oncology. All the most innovative processes of EVs engineering are discussed together with the related degree of applicability for each one of them in cancer nanomedicines.

show abstract

“…However, direct covalent modification of the proteins at the cell cytoskeleton can substantially affect cell viability and normal cell function, while hydrophobic insertion does not offer a permanent surface modification, with lipid–polymer conjugates rapidly dissociating from cells. [ 37,62,63 ] On the other end, introduction of bio‐orthogonal functionalities at the cell membrane offers clear advantages over nonspecific polymer conjugation, including better cytocompatibility and stability of conjugation. [ 64–66 ] The cell surface repertoire can be expanded to include abiotic functionality through the biosynthetic introduction of unnatural sugars into cellular glycans, a process termed metabolic oligosaccharide engineering (MOE).…”

Section: Covalent Conjugation Using Bio‐orthogonal Chemistrymentioning

confidence: 99%

Engineering the Mammalian Cell Surface with Synthetic Polymers: Strategies and Applications

Arno

2020

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Manipulating the surface of living cells represents a powerful tool by which to control cell behavior and provides a unique strategy to modulate cellular function and cell–cell interactions. Recent progress in this area has seen the development of robust and elegant approaches to selectively decorate the cell surface, leading to unprecedented advances in cellular manipulation and cell‐based therapies. Despite some impressive in vitro results, several obstacles remain to the broader application of some of these strategies, including their limited translation in vivo. In this review, the leading techniques used to introduce polymers at the plasma membrane of mammalian cells are discussed, focusing on strategies that generate a stable and homogeneous distribution of polymeric chains at the cell surface. Application of these strategies to control cell behavior and deliver cell‐based therapies to targeted tissues are highlighted.

show abstract

Interaction of poly(ethylene glycol)-conjugated phospholipids with supported lipid membranes and their influence on protein adsorption

Cited by 39 publications

References 33 publications

Designer Exosomes for Active Targeted Chemo‐Photothermal Synergistic Tumor Therapy

Designer Exosomes for Active Targeted Chemo‐Photothermal Synergistic Tumor Therapy

Engineered Extracellular Vesicles as a Reliable Tool in Cancer Nanomedicine

Engineering the Mammalian Cell Surface with Synthetic Polymers: Strategies and Applications

Contact Info

Product

Resources

About