Interaction of plastocyanin with oligopeptides: effect of lysine distribution within the peptide

Hirota, Shun; Okumura, Hiroki; Arie, Sachiko; Tanaka, Kentaro; Shionoya, Mitsuhiko; Takabe, Teruhiro; Funasaki, Noriaki; Watanabe, Yoshihito

doi:10.1016/j.jinorgbio.2003.10.022

Cited by 1 publication

(2 citation statements)

References 48 publications

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“…[20,33] The largest CSPs for PoPc occurred for residues Asp44, Ser45, Asp51, Ile55, and Gln88. Among these residues, Asp44 belongs to the acidic patch.…”

Section: Backbone Assignmentsmentioning

confidence: 99%

“…In PoPc and DPc, most CSPs occurred around the regions with the acidic patches, in agreement with the assumption that the positively charged peptides interact with the acidic residues of Pc. [20,33] The largest CSPs for PoPc occurred for residues Asp44, Ser45, Asp51, Ile55, and Gln88. Among these residues, Asp44 belongs to the acidic patch.…”

Section: Backbone Assignmentsmentioning

confidence: 99%

See 1 more Smart Citation

An Ensemble of Rapidly Interconverting Orientations in Electrostatic Protein–Peptide Complexes Characterized by NMR Spectroscopy

et al. 2014

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Protein complex formation involves an encounter state in which the proteins are associated in a nonspecific manner and often stabilized by interactions between charged surface patches. Such patches are thought to bind in many different orientations with similar affinity. To obtain experimental evidence for the dynamics in encounter complexes, a model was created using the electron transfer protein plastocyanin and short charged peptides. Three plastocyanins with distinct surface charge distributions were studied. The experimental results from chemical shift perturbations, paramagnetic relaxation enhancement (PRE) NMR, and theoretical results from Monte Carlo simulations indicate the presence of multiple binding orientations that interconvert quickly and are dominated by long-range charge interactions. The PRE data also suggest the presence of highly transient orientations stabilized by short-range interactions.

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“…[20,33] The largest CSPs for PoPc occurred for residues Asp44, Ser45, Asp51, Ile55, and Gln88. Among these residues, Asp44 belongs to the acidic patch.…”

Section: Backbone Assignmentsmentioning

confidence: 99%

Section: Backbone Assignmentsmentioning

confidence: 99%