Interaction of pepsin with aromatic amino acids and their derivatives immobilized to Sepharose

Vinyl Sulfone: A Multi-Purpose Function in Proteomics

“…Remarkably beads coupled to lectins or saccharides are currently used in glycomics (Kaji et al, 2003;Bunkenborg et al, 2004;Yang & Hancock, 2004). Vinyl sulfone activated agarose has been the bead of choice to study the interaction of pepsin with aromatic amino acids (Frydlova et al, 2004;Frydlova et al, 2008) or for the isolation of phophorylcholine-binding proteins (Liberda et al, 2002a) by affinity chromatography. To our knowledge the only work using vinyl sulfone activated sepharose that may resemble Proteomics is that published by Liberda et al, (Liberda et al, 2002b) who immobilized mannan to isolate mannan-binding bull seminal proteins that were identified by N-terminal amino acid sequencing.…”