Interaction of pathogenic neisseriae with nonphagocytic cells

Nassif, Xavier; So, Magdalene

doi:10.1128/cmr.8.3.376

Cited by 82 publications

(50 citation statements)

References 132 publications

(28 reference statements)

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“…The effects of all these on N. gonorrhoeae are not yet known, but it can resist destruction by defensins in part through a multiple drug exporter mechanism 51. In addition, intracellular residence within neutrophils,43 where it can resist intracellular killing mechanisms, or within epithelial cells,52 may enable it to escape from soluble anti-microbial factors.…”

Section: Discussionmentioning

confidence: 99%

Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection

et al. 2010

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Host immune responses, including the characteristic influx of neutrophils, against Neisseria gonorrhoeae are poorly understood; adaptive immunity is minimal and nonprotective. We hypothesize that N. gonorrhoeae selectively elicits Th17-dependent responses which recruit innate defense mechanisms including neutrophils and antimicrobial proteins that it can resist. We found that N. gonorrhoeae induced production of IL-17 in mouse T cells and of Th17-inducing cytokines in mouse and human antigen-presenting cells in vitro. IL-17 was induced in the iliac lymph nodes in vivo in a female mouse model of genital tract gonococcal infection. Antibody blockade of IL-17 or deletion of the major IL-17 receptor in IL-17RA-knockoutmice led to prolonged infection and diminished neutrophil influx. Genital tract tissue from IL-17RA-knockout mice showed reduced production of neutrophil-attractant chemokines in response to culture with N. gonorrhoeae. These results imply a crucial role for IL-17 and Th17 cells in the immune response to N. gonorrhoeae.

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Section: Discussionmentioning

confidence: 99%

Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection

et al. 2010

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“…Gonococcal attachment to human epithelial cells is mediated primarily by pili, surface structures composed of multiple units of the protein pilin (3,4). Pili mediate adherence to a number of different eukaryotic cell types, affect interactions with epithelia, endothelia and phagocytes (5), and are also required for full DNA transformation competence (6–8). …”

Section: Introductionmentioning

confidence: 99%

A real-time semi-quantitative RT-PCR assay demonstrates that the pilE sequence dictates the frequency and characteristics of pilin antigenic variation in Neisseria gonorrhoeae

Rohrer¹

2005

Nucleic Acids Research

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A semi-quantitative real-time RT–PCR assay was designed to measure gonococcal pilin antigenicvariation (SQ-PCR Av assay). This assay employs 17 hybridization probe sets that quantitate subpopulations of pilin transcripts carrying different silent pilin copy sequences and one set that detects total pilE transcript levels. Mixtures of a DNA standard carrying the silent copy being detected and a clone encoding the starting pilE sequence, which is the majority pilE template, provided amplification curves that closely matched the experimental data and allowed an analysis of the contribution of different silent pilin copies to variation. The SQ-PCR Av assay was verified using DNA sequence analysis to demonstrate that this methodology allowed an accurate analysis of pilin variation. Both assays showed that with a specific starting pilE sequence, only a subset of the silent pilin copies recombine into pilE at a detectable level, and that this limited subset was reproducibly detected in replicate cultures. When an isogenic pilE sequence variant was examined using both assays, a new subset of silent copy sequences were detected recombining into pilE and the overall frequency of variation was increased. Thus, the parental pilE sequence influences the frequency of variation and the repertoire of pilin variants produced.

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“…brospinal fluid (CSF) via the bloodstream and is therefore capaType IV pili are the major ligand mediating adhesion of ble of crossing the blood-brain barrier (BBB). First, N. menincapsulated meningococci to EC (reviewed in [5]). N. meningitgitidis can be recovered from the CSF of rhesus monkeys after idis pili fall into two classes.…”

mentioning

confidence: 99%

Interaction ofNeisseria meningitidiswith the Components of the Blood‐Brain Barrier Correlates with an Increased Expression of PilC

Pron¹,

Taha²,

Rambaud³

et al. 1997

J INFECT DIS

117

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A fatal untreated case of fulminant meningococcemia was examined to investigate the crossing of the blood-brain barrier (BBB) by Neisseria meningitidis. Microscopic examination showed bacteria in vivo adhering to the endothelium of both the choroid plexus and the meninges. Comparison of the isolates cultivated from the blood and the cerebrospinal fluid (CSF) revealed no antigenic variation of the pilin or the class 5 protein, whereas the expression of the PilC protein was greater in the CSF and the choroid plexus than in the blood. This was due to an increased activity of one of the pilC promotors. This higher expression of PilC correlated in vitro with greater adhesiveness to endothelial cells. A mutation in the single pilC locus of this strain abolished in vitro pilus-mediated adhesion to endothelial cells. These data suggest that PilC plays an important role in the crossing of the BBB, likely through pilus-mediated adhesion.

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Interaction of pathogenic neisseriae with nonphagocytic cells

Cited by 82 publications

References 132 publications

Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection

Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection

A real-time semi-quantitative RT-PCR assay demonstrates that the pilE sequence dictates the frequency and characteristics of pilin antigenic variation in Neisseria gonorrhoeae

Interaction ofNeisseria meningitidiswith the Components of the Blood‐Brain Barrier Correlates with an Increased Expression of PilC

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