Interaction of pancreatic colipase with a bile salt micelle

Charge shift electrophoresis: simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution.

“…18 strength is low. It should also be mentioned in this context that pancreatic colipase binds large amounts of deoxycholate and other negatively charged detergents (19,20), but it does not bind Triton X-100 (21) or positively charged deoxycholate derivatives (B. Borgstrom, unpublished observation). The use of three detergent systems (anionic, nonionic, and cationic) helps to detect such charge-specific binding.…”

The Promise of Enzymes in Therapy of Uremia. I. Theoretical Basis – Bowel Physiology

“…The breakdown of dietary fat into finely divided particles by lipases signifies thus the 1st stage of fat hydrolysis [6,8], This occurs in duodenum and jejunum, and secondarily in the proximal ileum, pancreatic lipases [6,8,11,18,34,35] and colipases [36,37] being the most im portant enzymes involved. The machinery of action of pancreatic lipases is largely dependent, viz.…”

“…Colipases are essential in the process, restoring in the presence of bile acid salts, both lipase activity and absorption. Presumably, colipase binds to a bile salt mole cule and serves as an anchor for pancreatic lipase at the substrate surface [36,37].…”

Pancreatic Colipase : Crystallographic and Biochemical Aspects

“…bile salt micelles [4] and other polar interfaces [S] has led to the hypothesis that this cofactor may act as an anchor for lipase in its attachment to highly polar interfaces.…”