Interaction of oxalate with β-glucan: Implications for the fungal extracellular matrix, and metabolite transport

Pérez-González, Gabriel; Tompsett, Geoffrey A.; Mastalerz, Kyle; Timko, Michaël T.; Goodell, Barry

doi:10.1016/j.isci.2023.106851

Cited by 4 publications

(1 citation statement)

References 68 publications

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“…Studies on wood decay have shown that oxalate can serve as a source of protons for the enzymatic and non-enzymatic hydrolysis of wood carbohydrates (47). In addition, oxalate can bind non-covalently to ꞵ-glucan and form a hydrogel in the extracellular matrix, which can influence the secretion of metabolites (48), which raises the possibility that these transcriptome changes influence cell wall functioning. Oxaloacetate is a precursor molecule of acetyl-CoA and other metabolites that are important for the biosynthesis of various compounds such as ergosterol and membrane lipids, which are essential for cell growth.…”

Section: Resultsmentioning

confidence: 99%

Snowball: a novel gene family required for developmental patterning in fruiting bodies of mushroom-forming fungi (Agaricomycetes)

Földi,

Merényi,

Balázs

et al. 2023

Preprint

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The morphogenesis of sexual fruiting bodies of fungi is a complex process determined by a genetically encoded program. Fruiting bodies reached the highest complexity levels in the Agaricomycetes, yet, the underlying genetics is currently poorly known. In this work, we functionally characterized a highly conserved unannotated gene termedsnb1, whose expression level increases rapidly during fruiting body initiation. According to phylogenetic analyses, orthologues ofsnb1are present in almost all agaricomycetes and may represent a novel conserved gene family that plays a substantial role in fruiting body development. We disruptedsnb1using CRISPR/Cas9 in the agaricomycete model organismCoprinopsis cinerea. Snb1deletion mutants formed unique, snowball-shaped, rudimentary fruiting bodies that could not differentiate caps, stipes and lamellae. We took advantage of this phenotype to study fruiting body differentiation using RNA-Seq analyses. This revealed differentially regulated genes and gene families that, based on wild-type RNA-Seq data, were upregulated early during development and showed tissue-specific expression, underscoring their potential role in differentiation. Taken together, the novel gene family ofsnb1and the differentially expressed genes in thesnb1mutants provide valuable insights into the complex mechanisms underlying developmental patterning in the Agaricomycetes.ImportanceFruiting bodies of mushroom-forming fungi (Agaricomycetes) are complex multicellular structures, with a spatially and temporally integrated developmental program that is, however, currently poorly known. In this study we present a novel, conserved gene family, Snowball (snb), termed after the unique, differentiation-less fruiting body morphology ofsnb1knockout strains in the model mushroomCoprinopsis cinerea. Snbis a hitherto unannotated gene that is highly conserved among agaricomycetes and encodes a protein of unknown function. A comparative transcriptomic analysis of the early developmental stages of differentiated wild-type and non-differentiated mutant fruiting bodies revealed conserved differentially expressed genes which may be related to tissue differentiation and developmental patterning fruiting body development.

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Section: Resultsmentioning

confidence: 99%

Snowball: a novel gene family required for developmental patterning in fruiting bodies of mushroom-forming fungi (Agaricomycetes)

Földi,

Merényi,

Balázs

et al. 2023

Preprint

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Role of oxalic acid in fungal and bacterial metabolism and its biotechnological potential

Grąz

2024

World J Microbiol Biotechnol

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Oxalic acid and oxalates are secondary metabolites secreted to the surrounding environment by fungi, bacteria, and plants. Oxalates are linked to a variety of processes in soil, e.g. nutrient availability, weathering of minerals, or precipitation of metal oxalates. Oxalates are also mentioned among low-molecular weight compounds involved indirectly in the degradation of the lignocellulose complex by fungi, which are considered to be the most effective degraders of wood. The active regulation of the oxalic acid concentration is linked with enzymatic activities; hence, the biochemistry of microbial biosynthesis and degradation of oxalic acid has also been presented. The potential of microorganisms for oxalotrophy and the ability of microbial enzymes to degrade oxalates are important factors that can be used in the prevention of kidney stone, as a diagnostic tool for determination of oxalic acid content, as an antifungal factor against plant pathogenic fungi, or even in efforts to improve the quality of edible plants. The potential role of fungi and their interaction with bacteria in the oxalate-carbonate pathway are regarded as an effective way for the transfer of atmospheric carbon dioxide into calcium carbonate as a carbon reservoir.

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Snowball: a novel gene family required for developmental patterning of fruiting bodies of mushroom-forming fungi (Agaricomycetes)

Földi,

Merényi,

Balázs

et al. 2024

mSystems

View full text Add to dashboard Cite

Fruiting bodies of mushroom-forming fungi (Agaricomycetes) are complex multicellular structures, with a spatially and temporally integrated developmental program that is, however, currently poorly known. In this study, we present a novel, conserved gene family, Snowball (snb), termed after the unique, differentiation-less fruiting body morphology of snb1 knockout strains in the model mushroom Coprinopsis cinerea. snb is a gene of unknown function that is highly conserved among agaricomycetes and encodes a protein of unknown function. A comparative transcriptomic analysis of the early developmental stages of differentiated wild-type and non-differentiated mutant fruiting bodies revealed conserved differentially expressed genes which may be related to tissue differentiation and developmental patterning fruiting body development.

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Interaction of oxalate with β-glucan: Implications for the fungal extracellular matrix, and metabolite transport

Cited by 4 publications

References 68 publications

Snowball: a novel gene family required for developmental patterning in fruiting bodies of mushroom-forming fungi (Agaricomycetes)

Snowball: a novel gene family required for developmental patterning in fruiting bodies of mushroom-forming fungi (Agaricomycetes)

Role of oxalic acid in fungal and bacterial metabolism and its biotechnological potential

Snowball: a novel gene family required for developmental patterning of fruiting bodies of mushroom-forming fungi (Agaricomycetes)

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