Interaction of Oligonucleotide Derivativts with Animal Cells

Vlassov, V. V.; Deeva, Elena A.; M, Nechaeva; E, Rykova; Yakubov, L. A.

doi:10.1080/07328319108046535

Cited by 6 publications

(5 citation statements)

References 1 publication

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“…Taking into account the existence of specific nucleic acid binding cellular receptors [6,8] we suggest that the mechanism of oligonucleotide penetration be transcytosis through the mucosa cells. This makes one optimistic about the prospects of the in vivo use of natural oligonucleotides.…”

Section: Resultsmentioning

confidence: 99%

“…The described experiments evidence that the oligonucleotides can penetrate into mice through the nasal, rectal and vaginal mucosa and even through the skin. Taking into account the existence of specific nucleic acid binding cellular receptors [6,8] we suggest that the mechanism of oligonucleotide penetration be transcytosis through the mucosa cells. This makes one optimistic about the prospects of the in vivo use of natural oligonucleotides.…”

Section: Febslettersmentioning

confidence: 99%

“…The radioactivity of the blood samples (odd-numbered lanes on the gel) and pancreas samples (even-numbered lanes) was analyzed by electrophoresis under denaturing conditions (20% PAAG, 7 M urea). (1,2) intranasal administration(3,4) intravaginal administration; (56) administration per OS;(7,8) i.p. injection.…”

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confidence: 99%

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Penetration of oligonucleotides into mouse organism through mucosa and skin

1993

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Benzylamide 5'-32P-oligonucleotide derivatives were shown to penetrate into mice organism when administered by various routes: intranasally, per OS, intravaginally and per rectum. In all cases, the compounds are rapidly accumulated in blood and guts. Analysis of the radioactive material from blood and pancreas revealed intact oligonucleotides. Although concentrations of oligonucleotides in tissues differ considerably by the various methods of administration, the efficiency of delivery is sufficient to consider all the routes as being of therapeutic value. Dose effect on the efficiency of oligonucleotide penetration into mice suggests the transport to be a saturable process. Application of an oligonucleotide lotion on mice ear helices results in reproducible accumulation of radioactivity in the animal tissues. Effectiveness of oligonucleotide delivery into mouse through skin can be improved by using electrophoretic procedure.

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Section: Resultsmentioning

confidence: 99%

Section: Febslettersmentioning

confidence: 99%

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Penetration of oligonucleotides into mouse organism through mucosa and skin

1993

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“…It was the only one labeled in mouse hepatocytes. The K d for hepatocyte receptors was also estimated to be 0.2 µM35.…”

Section: Plasma Membrane Receptors For Oligonucleic Acidsmentioning

confidence: 99%

Hypothesis: naked plasmid DNA is taken up by cellsin vivo by a receptor-mediated process

et al. 2000

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“…The investigation was performed with the murine fibroblast L929 cell line, which was selected for experiments as these cells have been shown to contain a surface 79 kDa oligonucleotide-binding protein (putative receptor) and the protein is present in greater amounts as compared with other cell lines tested [7,8] 10), were synthesized using the triester method in solution in the Novosibirsk Institute of Bioorganic Chemistry as described in [9], purified by reverse-phase HPLC, precipitated with lithium perchlorate in acetone, washed with acetone and kept dry. The investigation was performed with the murine fibroblast L929 cell line, which was selected for experiments as these cells have been shown to contain a surface 79 kDa oligonucleotide-binding protein (putative receptor) and the protein is present in greater amounts as compared with other cell lines tested [7,8] 10), were synthesized using the triester method in solution in the Novosibirsk Institute of Bioorganic Chemistry as described in [9], purified by reverse-phase HPLC, precipitated with lithium perchlorate in acetone, washed with acetone and kept dry.…”

Section: Introductionmentioning

confidence: 99%

Rapid sequence‐independent cellular response to oligodeoxynucleotides

et al. 1997

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The presence of receptors for oligodeoxynucleotides (OdN) on the surface of L929 cells has previously been described. To study the possible coupling of the receptor to cellular signal transducing systems, the effect of phosphodiester OdN of different sequences on cellular phospholipase C and protein kinase C (PKC) activities in L929 fibroblasts was studied. Treatment of cells with OdN induced an increase in 32P labeling of phosphatidic acid which was accompanied by a gradual decrease in diacylglycerol. These effects seem to be independent of the OdN sequence. PKC activity in membranes isolated from OdN-treated cells was found to be lower than that in membranes of control cells. SDS-PAGE of the 32P-labeled cellular proteins revealed that OdN treatment caused a decrease in phosphorylation of the 26 and 73 kDa cellular proteins in the cells.

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Interaction of Oligonucleotide Derivativts with Animal Cells

Cited by 6 publications

References 1 publication

Penetration of oligonucleotides into mouse organism through mucosa and skin

Penetration of oligonucleotides into mouse organism through mucosa and skin

Hypothesis: naked plasmid DNA is taken up by cellsin vivo by a receptor-mediated process

Rapid sequence‐independent cellular response to oligodeoxynucleotides

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