Interaction of neutral polysaccharides with phosphatidylcholine multilamellar liposomes. Phase transitions studied by the binding of fluorescein-conjugated dextrans

Minetti, Maurizio; Aducci, Patrizia; Viti, Vincenza

doi:10.1021/bi00579a017

Cited by 30 publications

(14 citation statements)

References 37 publications

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“…It is widely known that neutral polysaccharides chemically interact with cell membranes (12). They have been shown to induce cell aggregation (18).…”

Section: Discussionmentioning

confidence: 99%

“…They have been shown to induce cell aggregation (18). Minetti et aL (12) suggested that a direct adsorptive binding between dextrans and phospholipids occurs that induces an altered phospholipid packing order. The exact molecular mechanisms of these effects are unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The purposes of this investigation were to determine whether the observed membrane effects of P188 on isolated cells were relatively specific to its molecular properties by comparing P188 with a neutral polysaccharide known to adsorb on the lipid bilayer (12), to determine whether the P188 and neutral polysaccharide would also reach damaged cell membranes in situ via intravenous administration and seal them after electropermeabilization, and, most important, to determine whether membrane sealing could prevent tissue necrosis following electrical injury. …”

Section: Introductionmentioning

confidence: 99%

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Surfactant-induced sealing of electropermeabilized skeletal muscle membranes in vivo.

Lee

River

Pan

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

289

251

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Victims of major electrical trauma frequently suffer extensive skeletal muscle and nerve damage, which is postulated to be principally mediated by electroporation and/or thermally driven cell membrane permeabilization. We have investigated the efficacy oftwo blood-compatible chemical surfactants for sealing electroporated muscle membranes. In studies using cultured skeletal muscle cells, poloxamer 188 (P188; an 8. Membrane damage is often manifested clinically by release of intracellular contents into the intravascular space (5), one of the clinical hallmarks of major electrical trauma. Skeletal muscle and peripheral nerve necrosis appears to be the primary cause of the high amputation rates associated with electrical trauma. We have postulated that, in the majority of victims, cell membrane permeabilization is the most important pathophysiologic event leading to tissue death (4,6,7) and, therefore, effective therapy for victims of electric shock must reestablish cell membrane structural integrity.Because membranes form spontaneously when surfactants (amphiphiles) are mixed in an aqueous solvent at sufficient concentration, we hypothesized that it may be possible to seal damaged cell membranes by exposing them to adequate concentrations of a noncytotoxic nonionic surfactant, possibly by incorporation of the surfactant into the membrane defects. In a preliminary test of this concept, we found that an 8.4-kDa nonionic synthetic surfactant, poloxamer 188 (P188), which has been clinically accepted for human intravenous administration, effectively sealed electroporated membranes of cultured skeletal muscle cells when used in concentrations >0.5 mg/ml (8, 9). We also noted that sealing the membrane enhanced cell survival as measured by vital dye [i.e., carboxyfluorescein (CF) and trypan blue] assays. The ability of P188 to bind to damaged membranes has been suggested in previous studies (10, 11).The purposes of this investigation were to determine whether the observed membrane effects of P188 on isolated cells were relatively specific to its molecular properties by comparing P188 with a neutral polysaccharide known to adsorb on the lipid bilayer (12), to determine whether the P188 and neutral polysaccharide would also reach damaged cell membranes in situ via intravenous administration and seal them after electropermeabilization, and, most important, to determine whether membrane sealing could prevent tissue necrosis following electrical injury. MATERIALS AND METHODS

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“…It is widely known that neutral polysaccharides chemically interact with cell membranes (12). They have been shown to induce cell aggregation (18).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Surfactant-induced sealing of electropermeabilized skeletal muscle membranes in vivo.

Lee

River

Pan

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

289

251

View full text Add to dashboard Cite

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“…Dextrans may stop the development of low-pH-induced small defects into hemolytic holes since there is evidence that the dextran molecules penetrate between the carbohydrate groups on the red cell surface [4] and that they interact with phospholipid membranes [5][6][7]. The interaction of dextrans with phospholipid head groups [5] and carbohydrate groups [4] should result in a 'coating' of the red cell surface with dextrans [20,21]. Thus, the mechanism of dextran protection may be the interaction with incipient defects which is facilitated by the close proximity of dextrans to defect sites.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence was presented that dextrans penetrate between the carbohydrate groups on the red cell surface [4]. Dextran molecules also bind to the surface of phospholipid vesicles [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Dextran protection of erythrocytes from low‐pH‐induced hemolysis

et al. 1989

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Low-pH-induced hemolysis of erythrocytes is inhibited by dextrans. The protective effect was observed with dextrans larger than 40 kDa. Electron microscopy showed dextrans of 150 kDa in a tight association with the erythrocyte membrane. These results indicate that dextrans stop the low-pH-induced hemolysis by interacting with the acid-induced defects in the erythrocyte membrane [(1989) Biochim. Biophys. Acta, in press].

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Relationship between conformation of polysaccharides in the dilute regime and their interaction with a phospholipid bilayer

et al. 2001

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Interactions between polysaccharides and phospholipid bilayers have already been demonstrated in the literature but little is known about the influence of macromolecule conformations related to the solvent characteristics (pH, ions, ionic strength). In this study we have investigated the conformation of iono- and thermo-sensitive polysaccharides, iota- and kappa-carrageenans, and their interaction with a dimyristoylphosphatidylcholine (DMPC) model bilayer. The study was performed in two different media (NaCl 150 mmol/L, pH 6.5, and NaCl 300 mmol/L, pH 6.5). In the first part, the iota- and kappa-carrageenan samples have been characterized by size exclusion chromatography (SEC) coupled with a multi-angle laser light-scattering detector (MALLS). The SEC-MALLS results clearly show polysaccharide chain association at high ionic strength. In the second part, the polysaccharide-membrane interaction has been studied, using fluorescent probes embedded in the membrane. The thermotropic properties of the membrane were investigated by fluorescence depolarization of 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). The membrane surface accessibility was evaluated by fluorescence quenching of 2-(9-anthroyloxy) stearic acid (2-AS). Whatever the ionic strength tested, the polysaccharide presence notably enhances the membrane fluidity below the T(m). This sign of an interaction in the polar level of the membrane is more marked at low NaCl concentration. In contrast, the liposomes bilayer accessibility is drastically lowered when increasing the ionic strength. This is induced by macromolecular chain adsorption on the liposome surface, enhanced by the polysaccharide chain association. An ionic strength enhancement induces a conformational modification of the polysaccharide chains which modifies their ability to interact with the bilayer.

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Interaction of neutral polysaccharides with phosphatidylcholine multilamellar liposomes. Phase transitions studied by the binding of fluorescein-conjugated dextrans

Cited by 30 publications

References 37 publications

Surfactant-induced sealing of electropermeabilized skeletal muscle membranes in vivo.

Surfactant-induced sealing of electropermeabilized skeletal muscle membranes in vivo.

Dextran protection of erythrocytes from low‐pH‐induced hemolysis

Relationship between conformation of polysaccharides in the dilute regime and their interaction with a phospholipid bilayer

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Interaction of neutral polysaccharides with phosphatidylcholine multilamellar liposomes. Phase transitions studied by the binding of fluorescein-conjugated dextrans

Cited by 30 publications

References 37 publications

Surfactant-induced sealing of electropermeabilized skeletal muscle membranes in vivo.

Surfactant-induced sealing of electropermeabilized skeletal muscle membranes in vivo.

Dextran protection of erythrocytes from low‐pH‐induced hemolysis

Relationship between conformation of polysaccharides ­in the dilute regime and their interaction with a phospholipid bilayer

Contact Info

Product

Resources

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Relationship between conformation of polysaccharides in the dilute regime and their interaction with a phospholipid bilayer