Interaction of Nanoparticles with Lipid Monolayers and Lung Surfactant Films

Dwivedi, Mridula; Sachan, Amit Kumar; Galla, Hans Joachim

doi:10.1007/11663_2014_9

Cited by 4 publications

(2 citation statements)

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“…The surfactant is crucial for reducing the surface tension in alveoli. NPs that react with the surfactant alter its biophysical properties leading to impaired lung functioning (Dwivedi et al, 2014). Alveolar macrophages are removing NPs by phagocytosis from the alveolar space.…”

Section: Introductionmentioning

confidence: 99%

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

et al. 2017

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The pulmonary delivery of nanoparticles (NPs) is a promising approach in nanomedicine. For the efficient and safe use of inhalable NPs, understanding of NP interference with lung surfactant metabolism is needed. Lung surfactant is predominantly a phospholipid substance, synthesized in alveolar type II cells (ATII), where it is packed in special organelles, lamellar bodies (LBs). In vitro and in vivo studies have reported NPs impact on surfactant homeostasis, but this phenomenon has not yet been sufficiently examined. We showed that in ATII-like A549 human lung cancer cells, silica-coated superparamagnetic iron oxide NPs (SiO 2 -SPIONs), which have a high potential in medicine, caused an increased cellular amount of acid organelles and phospholipids. In SiO 2 -SPION treated cells, we observed elevated cellular quantity of multivesicular bodies (MVBs), organelles involved in LB biogenesis. In spite of the results indicating increased surfactant production, the cellular quantity of LBs was surprisingly diminished and the majority of the remaining LBs were filled with SiO 2 -SPIONs. Additionally, LBs were detected inside abundant autophagic vacuoles (AVs) and obviously destined for degradation. We also observed time-and dose-dependent changes in mRNA expression for proteins involved in lipid metabolism. Our results demonstrate that non-cytotoxic concentrations of SiO 2 -SPIONs interfere with surfactant metabolism and LB biogenesis, leading to disturbed ability to reduce hypophase surface tension. To ensure the safe use of NPs for pulmonary delivery, we propose that potential NP interference with LB biogenesis is obligatorily taken into account. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

et al. 2017

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“…In order to have better understanding of interaction of soft NPs with lung surfactant, lipid vesicles are prepared [20]. Vesicles consist of a tiny structure containing fluid and a lipid bilayer encapsulate that fluid.…”

Section: Composition Of Vesiclesmentioning

confidence: 99%

Investigation of Interaction of Soft Nanoparticles based Vesicles with Lung Surfactant via Langmuir-Blodgett Trough and Quartz Crystal Microbalance Study

Azam¹

2018

J Nanomed Nanotechnol

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Effects of nanoparticles on the mechanical functioning of the lung

Arick

Choi

Kim

et al. 2015

Advances in Colloid and Interface Science

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Interaction of Nanoparticles with Lipid Monolayers and Lung Surfactant Films

Cited by 4 publications

References 72 publications

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Investigation of Interaction of Soft Nanoparticles based Vesicles with Lung Surfactant via Langmuir-Blodgett Trough and Quartz Crystal Microbalance Study

Effects of nanoparticles on the mechanical functioning of the lung

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Interaction of Nanoparticles with Lipid Monolayers and Lung Surfactant Films

Cited by 4 publications

References 72 publications

Harmful at non-cytotoxic concentrations: SiO2-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Harmful at non-cytotoxic concentrations: SiO2-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Investigation of Interaction of Soft Nanoparticles based Vesicles with Lung Surfactant via Langmuir-Blodgett Trough and Quartz Crystal Microbalance Study

Effects of nanoparticles on the mechanical functioning of the lung

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Product

Resources

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Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells