Interaction of N-3-oxododecanoyl homoserine lactone with transcriptional regulator LasR of Pseudomonas aeruginosa: Insights from molecular docking and dynamics simulations
Abstract:Background: In 2017, the World Health Organization announced a list of the most dangerous superbugs. Among them is Pseudomonas aeruginosa, an opportunistic human pathogen with high levels of resistance to antibiotics that is listed as one of the ‘ESKAPE’ pathogens, which are the leading cause of nosocomial infections. A major issue is that it mostly affects vulnerable patients such as those suffering from AIDS, cystic fibrosis, cancer and severe burns. P. aeruginosa creates and inhabits surface-associated biof… Show more
“… 1 This microorganism uses several virulence factors to aid in its pathogenicity that will favor infection. 2 Among these factors one can highlight the production of biofilm, which is configured as a set of bacterial cells coated by polysaccharide layer, acting as a protection factor of the bacteria against the attack of the antimicrobial and host immune system, favoring the chronicity of the infection. 1 …”
Section: Introductionmentioning
confidence: 99%
“…The formation of biofilms causes considerable problems in the medical and industrial area, as these structures provoke greater resistance to the treatment with antibiotics and biocides, besides reducing the action of the host immune responses. 2 Due to its complexity and the impact of its development, microbial biofilms have been the subject of several studies trying to elucidate how these structures are formed and managed to interact within this community to mitigate the consequences of their formation. 3 …”
The modeling of the resulting LasR protein showed a conformational change in its structure, suggesting that this might be the reason why these isolates are unable to produce biofilm.
“… 1 This microorganism uses several virulence factors to aid in its pathogenicity that will favor infection. 2 Among these factors one can highlight the production of biofilm, which is configured as a set of bacterial cells coated by polysaccharide layer, acting as a protection factor of the bacteria against the attack of the antimicrobial and host immune system, favoring the chronicity of the infection. 1 …”
Section: Introductionmentioning
confidence: 99%
“…The formation of biofilms causes considerable problems in the medical and industrial area, as these structures provoke greater resistance to the treatment with antibiotics and biocides, besides reducing the action of the host immune responses. 2 Due to its complexity and the impact of its development, microbial biofilms have been the subject of several studies trying to elucidate how these structures are formed and managed to interact within this community to mitigate the consequences of their formation. 3 …”
The modeling of the resulting LasR protein showed a conformational change in its structure, suggesting that this might be the reason why these isolates are unable to produce biofilm.
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