Interaction of Mitochondrial and Epigenetic Regulation in Hepatocellular Carcinoma

Sánchez, Victoria Chagoya de; Chávez, Edgar; Loyden, Gabriela Velasco; Lozano-Rosas, María Guadalupe; RusbelAparicio-Cadena, Alejandro

doi:10.5772/intechopen.79923

Cited by 2 publications

(2 citation statements)

References 73 publications

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“…Thus, according to the cytological characteristics, it can be noted that HepG2 cells occupy an intermediate state between normal hepatocytes and tumor cells, for which significant changes in the epigenetic regulation of nuclear and mitochondrial genes are observed [ 81 , 82 ].…”

Section: Comparison Of Hepg2 Normal Hepatocyte Hb and Hccmentioning

confidence: 99%

The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

Arzumanian

Kiseleva

Poverennaya

2021

IJMS

143

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Liver cancer is the third leading cause of cancer death worldwide. Representing such a dramatic impact on our lives, liver cancer is a significant public health concern. Sustainable and reliable methods for preventing and treating liver cancer require fundamental research on its molecular mechanisms. Cell lines are treated as in vitro equivalents of tumor tissues, making them a must-have for basic research on the nature of cancer. According to recent discoveries, certified cell lines retain most genetic properties of the original tumor and mimic its microenvironment. On the other hand, modern technologies allowing the deepest level of detail in omics landscapes have shown significant differences even between samples of the same cell line due to cross- and mycoplasma infection. This and other observations suggest that, in some cases, cell cultures are not suitable as cancer models, with limited predictive value for the effectiveness of new treatments. HepG2 is a popular hepatic cell line. It is used in a wide range of studies, from the oncogenesis to the cytotoxicity of substances on the liver. In this regard, we set out to collect up-to-date information on the HepG2 cell line to assess whether the level of heterogeneity of the cell line allows in vitro biomedical studies as a model with guaranteed production and quality.

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Section: Comparison Of Hepg2 Normal Hepatocyte Hb and Hccmentioning

confidence: 99%

The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

Arzumanian

Kiseleva

Poverennaya

2021

IJMS

143

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“…These findings highlight a critical role of MATα1 in regulating mitochondrial function and could provide a novel target for the treatment of different liver diseases where mitochondrial dysfunction plays a key role. Taken together, reduced mitochondrial MATα1 could play a key role in the mitochondrial dysfunction that is often observed in HCC[104].…”

Section: Consequences Of Mat Genes Dysregulation In the Development Omentioning

confidence: 99%

Methionine adenosyltransferases in liver cancer

Murray¹,

Barbier-Torres²,

Fan³

et al. 2019

WJG

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Methionine adenosyltransferases (MATs) are essential enzymes for life as they produce S-adenosylmethionine (SAMe), the biological methyl donor required for a plethora of reactions within the cell. Mammalian systems express two genes, MAT1A and MAT2A , which encode for MATα1 and MATα2, the catalytic subunits of the MAT isoenzymes, respectively. A third gene MAT2B , encodes a regulatory subunit known as MATβ which controls the activity of MATα2. MAT1A , which is mainly expressed in hepatocytes, maintains the differentiated state of these cells, whilst MAT2A and MAT2B are expressed in extrahepatic tissues as well as non-parenchymal cells of the liver ( e.g ., hepatic stellate and Kupffer cells). The biosynthesis of SAMe is impaired in patients with chronic liver disease and liver cancer due to decreased expression and inactivation of MATα1. A switch from MAT1A to MAT2A/MAT2B occurs in multiple liver diseases and during liver growth and dedifferentiation, but this change in the expression pattern of MATs results in reduced hepatic SAMe level. Decades of study have utilized the Mat1a -knockout (KO) mouse that spontaneously develops non-alcoholic steatohepatitis (NASH) and hepatocellular carcinoma (HCC) to elucidate a variety of mechanisms by which MAT proteins dysregulation contributes to liver carcinogenesis. An increasing volume of work indicates that MATs have SAMe-independent functions, distinct interactomes and multiple subcellular localizations. Here we aim to provide an overview of MAT biology including genes, isoenzymes and their regulation to provide the context for understanding consequences of their dysregulation. We will highlight recent breakthroughs in the field and underscore the importance of MAT’s in liver tumorigenesis as well as their potential as targets for cancer therapy.

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Interaction of Mitochondrial and Epigenetic Regulation in Hepatocellular Carcinoma

Cited by 2 publications

References 73 publications

The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

Methionine adenosyltransferases in liver cancer

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