Interaction of Mengo virus with L cells

Brownstein, Barbara L.; Graham, Angus

doi:10.1016/0042-6822(61)90315-4

Cited by 40 publications

(13 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results (Table 1) indicate that comparable numbers of cells in mengovirusinfected cultures of both MDBK and L-cells produce and release virus. The efficiency-ofplating values reported in Table 1 for both host systems are consistent with previously reported values for mengovirus-infected L-cells which ranged from 0.35 to 0.80 (5). The low efficiencyof-plating values probably reflect the inefficient attachment of infected cells to the assay monolayers.…”

Section: Resultssupporting

confidence: 89%

“…These results would suggest that the host-related intracellular barriers to picornavirus replication limit to the same extent the expression of all the information of the input viral genome. In addition, the finding (6) that the pattern of host ' A preliminary report of this work was presented at the 69th Annual Meeting of the American Society for Microbiology, [4][5][6][7][8][9] May, 1969, Miami Beach, Fla. cell susceptibility to mengovirus differs from the pattern of host cells productively infected by Bovine enterovirus-1 suggests that the hostrelated intracellular barriers to picornavirus replication are quite specific. Since those cellular factors limiting, or stimulating, the replication of certain picornaviruses may also be control agents in normal cellular metabolism, the determination of the basis of picornavirus restriction may result in some understanding of the control of differential gene expression in normal cells.…”

mentioning

confidence: 99%

“…Plaques from virus titrations were counted after 48 to 60 hr by fixing and staining the monolayers with a solution of 1% crystal violet in 20% ethyl alcohol by the method of Buck et al (6). Determinations of infectious centers was performed by the method of Brownstein and Graham (5). Washed, infected cells were allowed to attach to L-cell monolayers for 2 hr prior to overlayering with 0.6% washed agar in MEM with 5% calf serum.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Host-Dependent Restriction of Mengovirus Replication

Wall

Taylor

1969

J Virol

View full text Add to dashboard Cite

Mengovirus infection of a restrictive cell line, Maden's bovine kidney (MDBK), results in a virus yield 1,000-fold less than that obtained from productively infected cell lines such as L cells or Ehrlich ascites tumor cells (EAT). Cells of both types of host systems are infected with comparable efficiencies and are completely killed as a consequence of infection. Infective center assays, coupled with the observation of total cell killing, suggest that comparable numbers of cells synthesize viral antigen and release virus in both types of host system. Viral-specific ribonucleic acid (RNA) synthesis is initiated and proceeds in an identical fashion for approximately 4 hr after the infection of MDBK, EAT, or L-cells. At this time, viral RNA synthesis in MDBK ceases, whereas viral RNA synthesis in EAT and L-cells continues at a linear rate. These results indicate that none of the early viral events leading to the initiation of viral-specific RNA synthesis constitutes the primary site of mengovirus restriction in MDBK. Rather it appears that the cessation of viral RNA synthesis in restrictive cells constitutes the primary limiting event. Based on its delayed interaction with mengovirus RNA synthesis, it appears that the host-related restrictive agent is initially compartmentalized and then released as a consequence of infection subsequent to those early events in mengovirus infection leading to the initiation and continued synthesis of viral RNA.

show abstract

Section: Resultssupporting

confidence: 89%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Host-Dependent Restriction of Mengovirus Replication

Wall

Taylor

1969

J Virol

View full text Add to dashboard Cite

show abstract

“…T h e two infecting agents employed are small R N A viruses belonging to the Columbia SK group. They are serologically related and both multiply to high titers in a common host, strain L mouse fibroblasts (4,8,14,17), cultured in suspension. This provided us with a suitable system for carrying out quantitative and morphological comparisons on virus multiplication and changes in the fine structure of the host.…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Changes in Fine Structure of L Cells During Single Cycles of Viral Multiplication, Following Their Infection With the Viruses of Mengo and Encephalomyocarditis

Dales

Franklin

1962

The Journal of Cell Biology

View full text Add to dashboard Cite

The virus of encephalomyocarditis (EMC), examined by the negative-contrast method, is indistinguishable from the serologically related Mengovirus. The particles are 270 to 280 A in diameter. The surface of EMC is composed of an undetermined number of subunits. Frequent sampling of infected cells was carried out throughout one-step cycles of viral multiplication to observe cytopathic changes occurring in L cells infected by these two related RNA viruses. EMC and Mengovirus, which multiply at equal rates, in most respects elicit similar alterations in cell fine structure. Rearrangement and changes in nuclear material accompanied by formation of small vesicles in the centrosphere region commence at 4 to 6 hours after infection. Thereafter a progressive degeneration of the nucleus and vesiculation of the cytoplasm are observed up to 18 to 20 hours. Increased numbers of small dense granules, indistinguishable from ribonucleoprotein particles, appear in the cytoplasm between 8 and 14 hours after infection. L cells infected with Mengovirus become permeable to Erythrocin more slowly than those infected with EMC. Only in the case of Mengovirus infection are large aggregates of dense material first observed in the cytoplasm at 8 hours, followed by the appearance of crystals probably composed of Mengovirus particles, at 12 hours. Differences in the rates of cell permeability after infection with EMC and Mengovirus are discussed in relation to formation of virus crystals and plaque-type mutants.

show abstract

“…McCarty et al (1964) suggested that Mycoplasma supplies infected cells with carbamyl phosphate, a substrate for the synthesis of pyrimidines. Brownstein and Graham (1961) reported that virus yields from infected cells are low compared with yields from noninfected cells and that infected cells are unusually fragile when inoculated with virus. We have also observed (unpublished data) that one Mycoplasma strain enhances CPE from a latent simian virus when it is added to African Green monkey kidney cells.…”

mentioning

confidence: 99%

Aerosols as a Source of Widespread Mycoplasma Contamination of Tissue Cultures1

O'Connell¹,

Wittler²,

Faber³

1964

Applied Microbiology

View full text Add to dashboard Cite

Aerosols as a source of widespread Mycoplasma contamination of tissue cultures. Appl. Microbiol. 12:337-342. 1964.-Mycoplasma isolates were cultured from 15 antibiotic-free cell cultures obtained from a single laboratory. Complement-fixation tests showed that these isolates were antigenically related to each other but were unrelated to M. hominis type 1, M. hominis type 2, M. arthritidis, Al. laidlawii type B, Mycoplasma sp. H.Ep. #2 (Barile), or Ml. salivarium. Examination of serum used to feed the infected cell lines revealed no Mycoplasma. Infection resulting from cross-contamination by a single Mycoplasma strain from one cell culture to another was investigated. Although the organisms were not found in the air over the work area, aerosols containing these contaminants were produced in tissue culture bottles during the trypsinization of cell monolayers. The minimal infectious dose of Mycoplasma for tissue cultures was measured, and it was determined that one organism was capable of initiating an infection in a tissue culture. The pattern of contamination and the small dose required for infection indicated that Mycoplasma contamination was spread from one tissue culture to another via aerosols. It was demonstrated that Mycoplasma can be transferred from one cell culture to another through the use of a common burette for dispensing medium.

show abstract

Interaction of Mengo virus with L cells

Cited by 40 publications

References 8 publications

Host-Dependent Restriction of Mengovirus Replication

Host-Dependent Restriction of Mengovirus Replication

A Comparison of the Changes in Fine Structure of L Cells During Single Cycles of Viral Multiplication, Following Their Infection With the Viruses of Mengo and Encephalomyocarditis

Aerosols as a Source of Widespread Mycoplasma Contamination of Tissue Cultures1

Contact Info

Product

Resources

About