Interaction of lipid vesicle with silver nanoparticle-serum albumin protein corona

Chen, Ran; Choudhary, Poonam; Schurr, Ryan N.; Bhattacharya, Priyanka; Brown, Jared M.; Ke, Pu Chun

doi:10.1063/1.3672035

Cited by 54 publications

(55 citation statements)

References 33 publications

(30 reference statements)

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“…In our case, the interaction of Ag NPs with BSA molecules resulted in a further change of e which resulted in a redshift of the extinction coefficient maximum, and the observed redshift in the SPR peaks. 17 Interestingly, Fig. 2(d) showed that the amount of redshift is dependent on the nature of surface groups present on the Ag NPs.…”

mentioning

confidence: 96%

Effects of surface functional groups on the formation of nanoparticle-protein corona

Podila

Chen

et al. 2012

Applied Physics Letters

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Herein, we examined the dependence of protein adsorption on the nanoparticle surface in the presence of functional groups. Our UV-visible spectrophotometry, transmission electron microscopy, infrared spectroscopy, and dynamic light scattering measurements evidently suggested that the functional groups play an important role in the formation of nanoparticle-protein corona. We found that uncoated and surfactant-free silver nanoparticles derived from a laser ablation process promoted a maximum protein (bovine serum albumin) coating due to increased changes in entropy. On the other hand, bovine serum albumin displayed a relatively lower affinity for electrostatically stabilized nanoparticles due to the constrained entropy changes.

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mentioning

confidence: 96%

Effects of surface functional groups on the formation of nanoparticle-protein corona

Podila

Chen

et al. 2012

Applied Physics Letters

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“…20 Third, changes in local membrane curvature followed by wrapping of NPs and their agglomerates may lead to lipid depletion from the bilayer, and thus altered membrane integrity and/ or formation of pores or holes. 16,22,[53][54][55][56][61][62][63][64] In the case of GUVs, we propose that the most likely mechanism causing GUVs to burst is adhesion of CAadsorbed CoFe 2 O 4 NPs and smaller NP agglomerates onto membranes, resulting in alteration of the global membrane curvature and, finally, bursting. The POPC lipids used in our study have an externally situated lipid polar head (the trimethylammonium group) that is positively charged and faces the aqueous glucose solution.…”

Section: Mlvs After Incubation In Np Suspensionsmentioning

confidence: 99%

“…11,12 Experimental, computational, and theoretical studies suggest that the NP-lipid interaction at the membrane can compromise the structure and function of both artificial and biological membranes. 1,[12][13][14][15][16] NPs and their agglomerates can be adsorbed onto the membrane and cause its structural reorganization, [13][14][15][16] leading to changes in membrane curvature, [17][18][19] particle internalization, 20 and pore and hole formation. 21,22 The interactions between lipid bilayers and solid surfaces are complex and can involve van der Waals, electrostatic, hydration, hydrophobic, or protrusion forces.…”

Section: Introductionmentioning

confidence: 99%

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Drašler¹,

Drobne²,

Novak³

et al. 2014

IJN

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Background The purpose of this work is to provide experimental evidence on the interactions of suspended nanoparticles with artificial or biological membranes and to assess the possibility of suspended nanoparticles interacting with the lipid component of biological membranes. Methods 1-Palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine (POPC) lipid vesicles and human red blood cells were incubated in suspensions of magnetic bare cobalt ferrite (CoFe 2 O 4 ) or citric acid (CA)-adsorbed CoFe 2 O 4 nanoparticles dispersed in phosphate-buffered saline and glucose solution. The stability of POPC giant unilamellar vesicles after incubation in the tested nanoparticle suspensions was assessed by phase-contrast light microscopy and analyzed with computer-aided imaging. Structural changes in the POPC multilamellar vesicles were assessed by small angle X-ray scattering, and the shape transformation of red blood cells after incubation in tested suspensions of nanoparticles was observed using scanning electron microscopy and sedimentation, agglutination, and hemolysis assays. Results Artificial lipid membranes were disturbed more by CA-adsorbed CoFe 2 O 4 nanoparticle suspensions than by bare CoFe 2 O 4 nanoparticle suspensions. CA-adsorbed CoFe 2 O 4 -CA nanoparticles caused more significant shape transformation in red blood cells than bare CoFe 2 O 4 nanoparticles. Conclusion Consistent with their smaller sized agglomerates, CA-adsorbed CoFe 2 O 4 nanoparticles demonstrate more pronounced effects on artificial and biological membranes. Larger agglomerates of nanoparticles were confirmed to be reactive against lipid membranes and thus not acceptable for use with red blood cells. This finding is significant with respect to the efficient and safe application of nanoparticles as medicinal agents.

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“…Among the diverse silver containing drugs that are currently used in medicine, viz., solutions, oint ments, alloys (lapis), colloidal silver, and nanoparti cles with different coats, the effect of the last form, which appeared only recently, has been studied most extensively. The available published data are sufficient to notice a significant difference in the membranotro pic effect of ionic and dispersed silver forms: the former is reported to have a tightening effect on the bilayer, whereas the latter causes its fluidization [13][14][15][16]. It was also shown that silver nanoparticles accu mulate in the cell wall and inside the cell [3,17,18].…”

Section: Introductionmentioning

confidence: 94%

The effects of silver nitrate on the phase state of model multibilayer membranes

et al. 2015

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To investigate the effects of silver nitrate (AgNO 3 ) on model lipid membranes, we studied multi bilayer membranes based on L α dipalmitoylphosphatidylcholine (DPPC) and AgNO 3 aqueous solutions in a wide concentration range (up to 30 wt %) using differential scanning calorimetry. It was shown that the pres ence of AgNO 3 leads to both an increase in the main phase transition temperature (T m ) of DPPC multibilay ers and the appearance of an additional phase transition peak ( ), suggesting an increase in both the density and heterogeneity of the lipid bilayer. Since the effect of nitrate ions ( ) was of the opposite nature (bilayer fluidizing), the integral effect of AgNO 3 can be explained solely by the effect of silver ions (Ag + ). With increasing AgNO 3 concentration, the tendency to opposite changes in T m and peak intensity was observed, so that at approximately 26 wt % AgNO 3 the initial peak (T m ) disappeared. In the range of thera peutic Ag + concentrations (up to 2 wt %), no significant changes in the phase state of model membranes were observed. This can be one of the causes of the absence of a damaging effect of silver containing drugs on the host cells against the background of a pronounced bactericidal effect.

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Interaction of lipid vesicle with silver nanoparticle-serum albumin protein corona

Cited by 54 publications

References 33 publications

Effects of surface functional groups on the formation of nanoparticle-protein corona

Effects of surface functional groups on the formation of nanoparticle-protein corona

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

The effects of silver nitrate on the phase state of model multibilayer membranes

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