Interaction of lectin-like proteins of South African medicinal plants with Staphylococcus aureus and Bacillus subtilis

Gaidamashvili, Mariam; Staden, J. Van

doi:10.1016/s0378-8741(02)00011-9

Cited by 39 publications

(28 citation statements)

References 8 publications

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“…Staphylococcus aureus and Bacillus subtilis (Gaidamashvili & van Staden, 2002); antihelmintic activity (McGaw et al, 2000) and potential benefits in treating coccidial infections (Naidoo et al, 2008).…”

Section: Tulbaghia Violaceamentioning

confidence: 99%

Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats

Raji

Mugabo

Obikeze

2012

Journal of Ethnopharmacology

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Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats I. A. RAJITulbaghia violacea Harv. (Alliaceae) is a small bulbous herb which belongs to the family, Alliaceae, most commonly associated with onions and garlic. In South Africa (SA), this herb has been traditionally used in the treatment of various ailments, including fever, colds, asthma, paralysis, hypertension (HTN) and stomach problems. The aim of this study was to evaluate the effect of methanol leaf extracts (MLE) of T. violacea on the blood pressure (BP) and heart rate (HR) in anaesthetized male spontaneously hypertensive rats; and to find out the mechanism(s) by which it acts.The MLE of T. violacea (5 -150 mg/kg), angiotensin I (ang I, 3.1 -100 µg/kg), captopril (10 mg/kg), angiotensin II (ang II, 3.1 -50 µg/kg), losartan (30 mg/kg), phenylephrine T. violacea (60 mg/kg) and captopril (10 mg/kg) were injected intraperitoneally into iii some SHR for 21 days to investigate the chronic effect of these agents on plasma levels of aldosterone. The mean change, the mean of the individual percentage changes and the percentage difference (in mean) observed with each intervention was calculated and statistically analyzed using the Student's t test for significant difference (p < 0.05). The Microsoft Excel software was used for statistical analysis.T. violacea significantly (p < 0.05) reduced the systolic, diastolic, and mean arterial BP;and HR dose-dependently. In a dose-dependent manner, ang I, ang II, phenylephrine significantly (p < 0.05) increased the BP, while propranolol, muscarine and atropine reduced the BP. The increases in BP due to dobutamine were not dose-dependent. In a dose dependent manner, phenylephrine and propranolol reduced the HR, while dobutamine increased the HR. The effect of ang I, ang II, muscarine and atropine on HR were not dose-dependent; with both increases as well as decreases observed with ang I, and II and atropine, while decreases were seen with muscarine. Captopril produced significant (p < 0.05) reduction in BP which were not associated with any change in HR.The co-infusion of ang I with the MLE produced significant (p < 0.05) reduction in BP, which were not associated with significant changes in HR. The co-infusion of ang II with the MLE did not produce any significant changes in BP or HR when compared to the infusion of the standard drug alone. The co-infusion of phenylephrine with the MLE did

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Section: Tulbaghia Violaceamentioning

confidence: 99%

Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats

Raji

Mugabo

Obikeze

2012

Journal of Ethnopharmacology

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“…Few reports have been published on the use of lectin or lectin-like substances from plants as inhibitors of fungal and bacterial growth (6)(7)(8)(9)(10)(11). Yet, the concentration of these proteins offers great potential for differential cell recognition (12).…”

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confidence: 99%

Differential activity of a lectin from Solieria filiformis against human pathogenic bacteria

Araújo

Melo

Silva

et al. 2005

Braz J Med Biol Res

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A lectin isolated from the red alga Solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/ bacterium). The lectin (500 µg/mL) stimulated the growth of the grampositive species Bacillus cereus and inhibited the growth of the gramnegative species Serratia marcescens, Salmonella typhi, Klebsiella pneumoniae, Enterobacter aerogenes, Proteus sp, and Pseudomonas aeruginosa at 1000 µg/mL but the lectin (10-1000 µg/mL) had no effect on the growth of the gram-positive bacteria Staphylococcus aureus and B. subtilis, or on the gram-negative bacteria Escherichia coli and Salmonella typhimurium. The purified lectin significantly reduced the cell density of gram-negative bacteria, although no changes in growth phases (log, exponential and of decline) were observed. It is possible that the interaction of S. filiformis lectin with the cell surface receptors of gram-negative bacteria promotes alterations in the flow of nutrients, which would explain the bacteriostatic effect. Growth stimulation of the gram-positive bacterium B. cereus was more marked in the presence of the lectin at a concentration of 1000 µg/mL. The stimulation of the growth of B. cereus was not observed when the lectin was previously incubated with mannan (125 µg/mL), its hapten. Thus, we suggest the involvement of the binding site of the lectin in this effect. The present study reports the first data on the inhibition and stimulation of pathogenic bacterial cells by marine alga lectins.

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“…This agglutination might have an effect on the bacterial multiplication as observed in some bacterial strains treated with plant agglutinin. In addition, other proteins can inhibit bacterial multiplication and motility (18). Electron microscopy scanning showed a formation of large vesicles in the bacterial cell membrane and, in some cases, bacterial cell membrane rupture.…”

Section: Discussionmentioning

confidence: 99%

“…These proteins (i.e., alboaggregin-B, echicetin, botrocetin, bitiscetin, flavocetin-A, aggretin/rhodocytin, convulxin and agkistin) provide new insight into platelet function (13,14). Other effects have also been reported for these proteins, including mitogenic activity on lymphocytes, release of cell calcium stores, inhibition of cell proliferation, renal effects and antimicrobial activity (15)(16)(17)(18). Previously, it has been observed that crotacetin from the venom of C. durissus had a significant inhibitory activity on two different bacterial strains, Xanthomonas axonopodis pv passiflorae and Clavibacter michiganensis michiganensis (19).…”

Section: Introductionmentioning

confidence: 99%

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

Barbosa¹,

Martins²,

Toyama³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis pv. passiflorae treated with lectin disclosed a high vesiculation and membrane rupture. BmLec induced a strong and significant increase in insulin secretion at 2.8 and 16.7 mM glucose concentrations, and this effect was seen in the presence of EGTA in both experiments. BmLec (10 µg/mL) increased the perfusion pressure, renal vascular resistance and urinary flow. The glomerular filtration rate and percentages of sodium, potassium and chloride tubular transport were reduced at 60 minutes of perfusion. Renal alterations caused by BmLec were completely inhibited by indomethacin in all evaluated parameters. In conclusion, the C-type lectin isolated from Bothrops moojeni affected platelet aggregation, insulin secretion, antibacterial activity and isolated kidney function

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Interaction of lectin-like proteins of South African medicinal plants with Staphylococcus aureus and Bacillus subtilis

Cited by 39 publications

References 8 publications

Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats

Effect of Tulbaghia violacea on the blood pressure and heart rate in male spontaneously hypertensive Wistar rats

Differential activity of a lectin from Solieria filiformis against human pathogenic bacteria

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

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