Interaction of Isoflurane with the Dopamine Transporter

Votaw, John R.; Byas-Smith, Michael G.; Hua, Jian; Voll, Ronald J.; Martarello, Laurent; Levey, Aĺlan I.; Bowman, Frederick O.; Goodman, Mark M.

doi:10.1097/00000542-200302000-00021

Cited by 56 publications

(42 citation statements)

References 24 publications

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“…There is much evidence that isoflurane moves the DAT into the cytoplasm so that it inhibits the binding of the DAT-specific tracers [26][27][28][29], which is not concordant with our result. The discordance could be explained by the diversity of the examined tissues (in vitro vs. in vivo studies and Rhesus monkeys vs. rats or mice) or by the different concentrations of isoflurane.…”

Section: Discussioncontrasting

confidence: 83%

Effect of Animal Condition and Fluvoxamine on the Result of [18F]N-3-Fluoropropyl-2β-carbomethoxy-3β-(4-iodophenyl) Nortropane ([18F]FP-CIT) PET Study in Mice

Shin

Park

Kim

et al. 2011

Nucl Med Mol Imaging

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Purpose PET (positron emission tomography) is a noninvasive imaging technique, visualizing biological aspects in vivo. In animal models, the result of PET study can be affected more prominently than in humans by the animal conditions or drug pretreatment. We assessed the effects of anesthesia, body temperature, and pretreatment with selective serotonin reuptake inhibitor on the results of18 F]FP-CIT PET of C57BL/6 mice was performed in three different conditions: (1) anesthesia (isoflurane) with active warming (38°C) as a reference; (2) no anesthesia or warming; (3) anesthesia without warming at room temperature. Additional groups of mice pretreated with escalating doses of fluvoxamine (5, 20, 40, 80 mg/kg) were imaged in condition (1). The time activity curve and standardized uptake value of the striatum, cerebral cortex, and bone were compared among these conditions. Results In all conditions, radioactivities of the striatum and cortex tended to form a plateau after rapid uptake and washout, but that of bone tended to increase gradually. When anesthetized without any warming, all the mice developed hypothermia and showed reduced bone uptake with slightly increased striatal and cortical uptakes compared to the reference condition. In conditions without anesthesia, striatal and cortical uptakes were reduced, whereas the bone uptake showed no change. Pretreatment with fluvoxamine increased the striatal uptake and striatal specific to cortical non-specific uptake ratio, whereas the bone uptake was reduced. Conclusion Anesthesia, body temperature, and fluvoxamine affect the result of [18 F]FP-CIT PET in mice by altering striatal and bone uptakes.

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Section: Discussioncontrasting

confidence: 83%

Effect of Animal Condition and Fluvoxamine on the Result of [18F]N-3-Fluoropropyl-2β-carbomethoxy-3β-(4-iodophenyl) Nortropane ([18F]FP-CIT) PET Study in Mice

Shin

Park

Kim

et al. 2011

Nucl Med Mol Imaging

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“…Thirdly, too few studies have been carried out on possible effects of anaesthetics on most aspects of CBF, brain metabolism and neurotransmission to provide a solid basis by which to judge the reliability of findings. 17,58 Ideally, confirmatory PET studies could be carried out in unanaesthetized, freely-moving animals, but that goal is currently out of reach in most cases.…”

Section: Discussion and Concluding Remarksmentioning

confidence: 99%

Anaesthesia for positron emission tomography scanning of animal brains

Alstrup

Smith

2013

Lab Anim

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Positron emission tomography (PET) provides a means of studying physiological and pharmacological processes as they occur in the living brain. Mice, rats, dogs, cats, pigs and non-human primates are often used in studies using PET. They are commonly anaesthetized with ketamine, propofol or isoflurane in order to prevent them from moving during the imaging procedure. The use of anaesthesia in PET studies suffers, however, from the drawback of possibly altering central neuromolecular mechanisms. As a result, PET findings obtained in anaesthetized animals may fail to correctly represent normal properties of the awake brain. Here, we review findings of PET studies carried out either in both awake and anaesthetized animals or in animals given at least two different anaesthetics. Such studies provide a means of estimating the extent to which anaesthesia affects the outcome of PET neuroimaging in animals. While no final conclusion can be drawn concerning the 'best' general anaesthetic for PET neuroimaging in laboratory animals, such studies provide findings that can enhance an understanding of neurobiological mechanisms in the living brain. A new era of neuroscience began with the invention of positron emission tomography (PET) for studying processes as they occur in the living brain.1 -4 PET makes use of the radioactive decay of positron-emitting nuclides to derive an image of physiological and pharmacological events in a living organ such as the brain. PET is currently the primary procedure for studying molecular events in realtime in intact animals and humans. PET scanning can be used in all branches of pharmacology, molecular biology and medicine, including neuroscience, cancer research and cardiovascular biology. 5 -7

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“…Ether exposure causes a massive expression of fos-like immunoreactivity in specific brain regions, including the basal ganglia and one of its main output nuclei, the substantia nigra pas compacta (Shehab & Julyan, 2002). Isoflurane, however, increases extracellular DA (Adachi et al, 2005), alters DA transporter (DAT) activity (Votaw et al, 2003) and induces hyperlocomotion (Irifune et al, 1998). In addition, studies of pattern generating spinal circuits in vitro explants of fetal or neonatal rat spinal cord have shown that isoflurane disrupts normal patterned activity (e.g., Wong, Sweitzer, Peters & Kendig, 2005).…”

Section: Discussionmentioning

confidence: 99%

Maternal anesthesia via isoflurane or ether differentially affects pre‐and postnatal behavior in rat offspring

Ronca

Abel

Alberts

2007

Developmental Psychobiology

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Our understanding of prenatal behavior has been significantly advanced by techniques for direct observation and manipulation of unanesthetized, behaving rodent fetuses with intact umbilical connections to the mother. These techniques involve brief administration of an inhalant anesthesic, enabling spinal transection of the rat or mouse dam, after which procedures can continue with unanesthetized dams and fetuses. Because anesthetics administered to the mother can cross the placental barrier, it is possible that fetuses are anesthetized to varying degrees. We compared in perinatal rats the effects of prenatal maternal exposure to two inhalant anesthetics: ether and isoflurane. Fewer spontaneous fetal movements and first postpartum nipple attachments were observed following maternal exposure to ether as compared to isoflurane. Neonatal breathing frequencies and oxygenation did not account for group differences in nipple attachment. Our results provide evidence that the particular inhalant anesthetic employed in prenatal manipulation studies determines frequencies of perinatal behavior.

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Interaction of Isoflurane with the Dopamine Transporter

Cited by 56 publications

References 24 publications

Effect of Animal Condition and Fluvoxamine on the Result of [18F]N-3-Fluoropropyl-2β-carbomethoxy-3β-(4-iodophenyl) Nortropane ([18F]FP-CIT) PET Study in Mice

Effect of Animal Condition and Fluvoxamine on the Result of [18F]N-3-Fluoropropyl-2β-carbomethoxy-3β-(4-iodophenyl) Nortropane ([18F]FP-CIT) PET Study in Mice

Anaesthesia for positron emission tomography scanning of animal brains

Maternal anesthesia via isoflurane or ether differentially affects pre‐and postnatal behavior in rat offspring

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