Interaction of iron polymers with blood mononuclear cells and its detection with the Prussian Blue reaction

Pons, Héctor; Sóyano, Andrés; Romano, Egidio

doi:10.1016/0162-3109(92)90006-x

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…To further verify the existence of the SPION in the tumor area, Prussian blue staining was carried out. − As shown in Figure , parts of the tumor tissues were stained blue, indicative of the presence or accumulation of iron oxide within the tumor areas. In good agreement with the above in vivo MRI images, larger amounts of iron oxide were observed in the case of SW-SPION than that of IS-SPION.…”

Section: Resultsmentioning

confidence: 99%

Antibiofouling Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as Potential Magnetic Resonance Contrast Agents for in Vivo Cancer Imaging

et al. 2006

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We report the fabrication and characterization of antifouling polymer-coated magnetic nanoparticles as nanoprobes for magnetic resonance (MR) contrast agents. Magnetite superparamagnetic iron oxide nanoparticles (SPION) were coated with the protein- or cell-resistant polymer, poly(TMSMA-r-PEGMA), to generate stable, protein-resistant MR probes. Coated magnetic nanoparticles synthesized using two different preparation methods (in situ and stepwise, respectively) were both well dispersed in PBS buffer at a variety of pH conditions (pH 1-10). In addition, dynamic light scattering data revealed that their sizes were not altered even after 24 h of incubation in 10% serum containing cell culture medium, indicative of a lack of protein adsorption on their surfaces. When the antibiofouling polymer-coated SPION were incubated with macrophage cells, uptake was significantly lower in comparison to that of the popular contrast agent, Feridex I.V., suggesting that the polymer-coated SPION can be long-circulated in plasma by escaping from uptake by the reticular endothelial system (RES) such as macrophages. Indeed, when the coated SPION were administered to tumor xenograft mice by intravenous injection, the tumor could be detected in T2-weighted MR images within 1 h as a result of the accumulation of the nanomagnets within the tumor site. Although the poly(TMSMA-r-PEGMA)-coated SPION do not have any targeting ligands on their surface, they are potentially useful for cancer diagnosis in vivo.

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Section: Resultsmentioning

confidence: 99%

Antibiofouling Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as Potential Magnetic Resonance Contrast Agents for in Vivo Cancer Imaging

et al. 2006

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“…Possibly, Fe(III)-citrate presumed to form a 1:1 or 1:2 aqueous complex may, in fact, form polynuclear Fe-citrate species (estimated at 72 Å), too large to diffuse into the periplasm (size limit estimated at 13 kDa ≈ 54 Å). Indeed, several studies pointed to the formation of polynuclear Fe(III) species in the presence of citrate at circumneutral pH, when the ratio of [Fe(III)]/[citrate] is close to stoichiometry. ,− The polynuclear species may also be too large to allow full access to the one MtrA solvent-exposed heme, resulting in slower reduction of ΔOMC as compared to WT. Abiotic reduction of Fe(III)-citrate by lysed cells rather than by outer membrane MHCs was ruled out up until 24 h because there was no detectable reduction, and cell viability only started decreasing radically after 24 h (Figure S2A).…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Reduction of Aqueous U(V)-dpaea and Solid-Phase U(VI)-dpaea Complexes: The Role of Multiheme c-Type Cytochromes

Molinas

Meibom

Faizova

et al. 2023

Environ. Sci. Technol.

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The biological reduction of soluble U(VI) complexes to form immobile U(IV) species has been proposed to remediate contaminated sites. It is well established that multiheme c-type cytochromes (MHCs) are key mediators of electron transfer to aqueous phase U(VI) complexes for bacteria such as Shewanella oneidensis MR-1. Recent studies have confirmed that the reduction proceeds via a first electron transfer forming pentavalent U(V) species that readily disproportionate. However, in the presence of the stabilizing aminocarboxylate ligand, dpaea 2− (dpaeaH 2 �bis(pyridyl-6-methyl-2-carboxylate)-ethylamine), biologically produced U(V) persisted in aqueous solution at pH 7. We aim to pinpoint the role of MHC in the reduction of U(V)-dpaea and to establish the mechanism of solidphase U(VI)-dpaea reduction. To that end, we investigated U-dpaea reduction by two deletion mutants of S. oneidensis MR-1−one lacking outer membrane MHCs and the other lacking all outer membrane MHCs and a transmembrane MHC−and by the purified outer membrane MHC, MtrC. Our results suggest that solid-phase U(VI)-dpaea is reduced primarily by outer membrane MHCs. Additionally, MtrC can directly transfer electrons to U(V)-dpaea to form U(IV) species but is not strictly necessary, underscoring the primary involvement of outer membrane MHCs in the reduction of this pentavalent U species but not excluding that of periplasmic MHCs.

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Inhibition of iron toxicity in rat and human hepatocyte cultures by the hydroxypyridin-4-ones CP20 and CP94

Chenoufi

Hubert

Loréal

et al. 1995

Journal of Hepatology

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Interaction of iron polymers with blood mononuclear cells and its detection with the Prussian Blue reaction

Cited by 4 publications

References 17 publications

Antibiofouling Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as Potential Magnetic Resonance Contrast Agents for in Vivo Cancer Imaging

Antibiofouling Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as Potential Magnetic Resonance Contrast Agents for in Vivo Cancer Imaging

Mechanism of Reduction of Aqueous U(V)-dpaea and Solid-Phase U(VI)-dpaea Complexes: The Role of Multiheme c-Type Cytochromes

Inhibition of iron toxicity in rat and human hepatocyte cultures by the hydroxypyridin-4-ones CP20 and CP94

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