Interaction of intraarticular hyaluronan and albumin in the attenuation of fluid drainage from joints

Scott, David A.; Coleman, Peter J.; Mason, Roger M.; Levick, J. R.

doi:10.1002/1529-0131(200005)43:5<1175::aid-anr28>3.0.co;2-b

Cited by 20 publications

(10 citation statements)

References 36 publications

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“…It should be noted here that the dissociation constant for the specific complex HA-HN, equal to 10 − 9 mol L − 1 (Maingonnat et al, 1999), is of the same order of magnitude as those for the non-specific complexes HA-BSA and HA-lysozyme, which are about 10 − 9 and 10 − 8 mol L − 1 respectively (Van Damme et al, 1994). Lysozyme is present in cartilage (Van Damme et al, 1994) and albumin is a major protein of synovial fluid (Scott et al, 2000), two areas which are also rich in HA. Knowing that the interactions involved in the complex HA-BSA (Xu et al, 2000) and in the complex HA-lysozyme (Van Damme et al, 1994) are electrostatic, and that a charge patch able to accommodate an HA decamer exists at the surface of human serum albumin (as Grymonpré et al (2001) have shown with integrated computer modeling), we suggest that the binding of HA to albumin and lysozyme might in fact be specific.…”

Section: Discussionmentioning

confidence: 63%

Hyaluronidase activity is modulated by complexing with various polyelectrolytes including hyaluronan

Deschrevel¹,

Lenormand²,

Tranchepain³

et al. 2008

Matrix Biology

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Section: Discussionmentioning

confidence: 63%

Hyaluronidase activity is modulated by complexing with various polyelectrolytes including hyaluronan

Deschrevel¹,

Lenormand²,

Tranchepain³

et al. 2008

Matrix Biology

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“…Further support for this assumption comes from the fact that convective fluid flow out of the joint cavity may be attenuated by SF osmotic pressure at high lubricant concentrations, and also by a concentration polarization phenomenon for large molecules in SF such as HA (McDonald and Levick, 1992;Sabaratnam et al, 2004;Scott et al, 2000). The secretion rates with no chemical stimulation will be considered to be basal levels.…”

Section: Of Synovial Fluid Lubricant Compositionmentioning

confidence: 99%

A model of synovial fluid lubricant composition in normal and injured joints

Blewis

Nugent-Derfus²,

Schmidt³

et al. 2007

eCM

122

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The synovial fluid (SF) of joints normally functions as a biological lubricant, providing low-friction and low-wear properties to articulating cartilage surfaces through the putative contributions of proteoglycan 4 (PRG4), hyaluronic acid (HA), and surface active phospholipids (SAPL). These lubricants are secreted by chondrocytes in articular cartilage and synoviocytes in synovium, and concentrated in the synovial space by the semi-permeable synovial lining. A deficiency in this lubricating system may contribute to the erosion of articulating cartilage surfaces in conditions of arthritis. A quantitative intercompartmental model was developed to predict in vivo SF lubricant concentration in the human knee joint. The model consists of a SF compartment that (a) is lined by cells of appropriate types, (b) is bound by a semi-permeable membrane, and (c) contains factors that regulate lubricant secretion. Lubricant concentration was predicted with different chemical regulators of chondrocyte and synoviocyte secretion, and also with therapeutic interventions of joint lavage and HA injection. The model predicted steady-state lubricant concentrations that were within physiologically observed ranges, and which were markedly altered with chemical regulation. The model also predicted that when starting from a zero lubricant concentration after joint lavage, PRG4 reaches steady-state concentration ~10-40 times faster than HA. Additionally, analysis of the clearance rate of HA after therapeutic injection into SF predicted that the majority of HA leaves the joint after ~1-2 days. This quantitative intercompartmental model allows integration of biophysical processes to identify both environmental factors and clinical therapies that affect SF lubricant composition in whole joints.

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“…1 and 4). (ii) Endothelium is the chief site of resistance to plasma protein permeation, and the reflection of plasma protein by the interstitial layer is negligible (Scott et al, 2000a). (iii) Studies using Monastral blue show the development of endothelial leaks (Poli et al, 2001).…”

Section: Does Cytochalasin Treatment Affect the Synovial Interstitialmentioning

confidence: 99%

Influence of Actin Cytoskeleton on Intra-articular and Interstitial Fluid Pressures in Synovial Joints

Poli

Scott

Bertin

et al. 2001

Microvascular Research

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Fibroblast microfilamentous actin (F-actin) influences interstitial fluid pressure via linkages to collagen in rat skin (Berg et al., 2001). The present aims were to determine whether the actin cytoskeleton of synovial endothelium, fibroblasts, and synoviocytes influences in vivo (i) fluid exchange between a joint cavity and synovial microcirculation and (ii) extracellular fluid pressures in joints. Rabbit knee joints were treated intra-articularly with the Factin disrupting drugs cytochalasin D and latrunculin B while joint fluid pressure P j was recorded. In joints injected with small volumes of control solution, P j fell with time (؊0.05 ؎ 0.01 cm H 2 O min ؊1 , mean ؎ SEM, n ‫؍‬ 9, equivalent drainage rate 3.9 l min ؊1 ). Cytochalasin or latrunculin reversed this in ϳ4 min in vivo; P j increased with time, e.g., ؉0.12 ؎ 0.04 cm H 2 O min ؊1 at 200 M cytochalasin (equivalent filtration rate into joint 6.6 -12.5 l min ؊1 , n ‫؍‬ 4), with a cytochalasin EC 50 of 45 M. Plasma ␥-globulin clearance into the joint cavity was also increased. Post mortem, cytochalasin did not reverse dP j /dt and had no more effect on P j than did control solution. Also, when synovial interstitial fluid pressures were measured by servonull micropipette post mortem (control ؊0.95 ؎ 0.37 cmH 2 O, n ‫؍‬ 18) cytochalasin had no significant effect on interstitial pressure over 60 min, even at 1 mM. It was concluded that synovial endothelial F-actin has an important role in the normal synovial microvascular resistance to fluid filtration and plasma ␥-globulin permeation and is thus a potential link between pro-in-

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Interaction of intraarticular hyaluronan and albumin in the attenuation of fluid drainage from joints

Cited by 20 publications

References 36 publications

Hyaluronidase activity is modulated by complexing with various polyelectrolytes including hyaluronan

Hyaluronidase activity is modulated by complexing with various polyelectrolytes including hyaluronan

A model of synovial fluid lubricant composition in normal and injured joints

Influence of Actin Cytoskeleton on Intra-articular and Interstitial Fluid Pressures in Synovial Joints

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