Interaction of Immobilized Avidin with an Aequorin–Biotin Conjugate: An Aequorin-Linked Assay for Biotin

Feltus, Agatha; Ramanathan, Sridhar; Daunert, Sylvia

doi:10.1006/abio.1997.2400

Cited by 23 publications

(9 citation statements)

References 45 publications

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“…Nanoparticles such as nanowires, nanotubes, and nanocrystals have been established as smart building blocks for emerging nanometer-sized electronic and sensing devices . Because of their very high aspect ratio, high electronic conductivity, and small size, nanowires and nanotubes offer potential advantages of high sensitivity, low power operation, and massive redundancy in nanosensor arrays.…”

Section: Introductionmentioning

confidence: 99%

Template Fabrication of Protein-Functionalized Gold−Polypyrrole−Gold Segmented Nanowires

et al. 2004

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Gold-capped, protein-modified polypyrrole (Ppy) nanowires were grown electrochemically using porous aluminum oxide as a template. The effects of the conditions of electrochemical synthesis on Ppy growth and protein (avidin or streptavidin) incorporation were studied. Streptavidin-modified nanowires grown at constant potential had better electrochemical properties and equilibrated faster when exposed to fluorescently labeled biotin than did nanowires grown by potential cycling. Solution pH had little effect on protein incorporation; however, higher pH provided slower but more reproducible growth rate of the Ppy segments. The best conditions for synthesis of streptavidin-modified Ppy nanowires were constant potential deposition at 0.75 V vs SCE in a phosphate buffer saline solution at pH 9. This method provides a straightforward route to nanowires of controlled length that can incorporate proteins for use in nanowire-based biosensors or in nanoparticle assembly through biomolecular interactions.

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Section: Introductionmentioning

confidence: 99%

Template Fabrication of Protein-Functionalized Gold−Polypyrrole−Gold Segmented Nanowires

et al. 2004

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“…Aequorin is a photoprotein native to the jellyfish Aequorea victoria that can be detected at levels as low as 10 -21 mol. , For this reason, it has been extensively used both in our laboratory and by others in the development of bioluminescence binding assays for various analytes, − including the vitamin biotin. , The biotin assay depends on competition between a biotin−aequorin conjugate (AEQ−biotin) and free biotin for biotin binding sites on the protein avidin. Binding of avidin to AEQ−biotin causes inhibition of the bioluminescence signal emitted by the photoprotein.…”

Section: Introductionmentioning

confidence: 99%

Detection of Biotin in Individual Sea Urchin Oocytes Using a Bioluminescence Binding Assay

et al. 2001

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The ability to detect biomolecules in single cells is important in order to fully understand the processes by which many biochemical events occur. To that end, we have developed a bioluminescence binding assay capable of measuring the intracellular biotin content of individual cells. The assay depends on competition between an aequorin-biotin conjugate (AEQ-biotin) and free biotin within the oocytes for binding sites on the protein avidin. The assay is performed by microinjecting each component into the oocytes and following the resulting bioluminescence within the oocyte upon triggering of aequorin. Results obtained using sea urchin oocytes show that the assay performed within the cells behaves in a manner consistent with assay theory. Using the assay, the individual biotin content of the oocytes is an average of approximately 20 amol. To our knowledge, this is the first reported multicomponent binding assay to be performed inside an intact single cell.

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“…It has been demonstrated that aequorin functions as a highly sensitive (attomole levels) label in the determination of both large (10 -14) and small biomolecules (15)(16). Obelin, thus far, has only been used in the development of immunoassays for the detection of large molecules such as IgG, and ␣-fetoproteins (17,18).…”

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confidence: 99%

Genetically Engineered Obelin as a Bioluminescent Label in an Assay for a Peptide

Матвеев

Lewis

Daunert

1999

Analytical Biochemistry

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Interaction of Immobilized Avidin with an Aequorin–Biotin Conjugate: An Aequorin-Linked Assay for Biotin

Cited by 23 publications

References 45 publications

Template Fabrication of Protein-Functionalized Gold−Polypyrrole−Gold Segmented Nanowires

Template Fabrication of Protein-Functionalized Gold−Polypyrrole−Gold Segmented Nanowires

Detection of Biotin in Individual Sea Urchin Oocytes Using a Bioluminescence Binding Assay

Genetically Engineered Obelin as a Bioluminescent Label in an Assay for a Peptide

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