Interaction of Human IgE with Fc Epsilon RI Alpha Exposes Hidden Epitopes on IgE

Abstract

Background: Binding of human IgE via the heavy–chain constant region domain 3 (Cε3) to the α–chain of its high affinity receptor (FcεRIα) is a key event in mediating allergic reactions. We wanted to identify epitopes within Cε3 that are stable to denaturation and to evaluate whether such structures are involved in receptor binding. The existence of stable epitopes would facilitate the generation of compounds that inhibit the IgE–FcεRIα interaction. Methods: Monoclonal anti–human IgE–antibodies against recombinant bacterially synthesized Cε3, which is known to be partly misfolded, were raised in mice. These antibodies were probed for binding to native, immobilized and receptor–bound IgE, respectively, providing tools for the identification of the indicated stable epitopes. Results: Two of the generated antibodies (8E7, 3G9) discriminate between IgE in solution and IgE attached to FcεRIα, pointing towards a steric rearrangement within Cε3 induced upon receptor binding. The described antibodies represent tools for studying the mechanism of the Fcε–FcεRIα interaction and may be of diagnostic value since serum IgE from various human donors was differently recognized by 8E7, which is indicative for naturally occurring IgE molecules with different steric conformation. Conclusion: The presented data support the hypothesis of a conformational change within IgE Cε3 upon receptor binding by showing that monoclonal antibodies raised against recombinant Cε3 differently recognize soluble and receptor–bound IgE. The presence of an IgE portion in sera of human donors that is recognized by 8E7 indicates the existence of IgE molecules in different steric conformations in human blood, which may be related to pathologic parameters.

Antikörper aus dem Gewächshaus

“…Ähnliche Wege beschreiten auch die Mitarbeiter bei Greenovation in Freiburg. Sie benutzen einen Bioreaktor mit Moos zur Erzeugung “vermenschlichter” Antikörper in Pflanzen 4)…”

Current Progress in the Understanding of IgE-FcεRI Interaction

“…Indeed any conformational consequence of antigen binding would be intriguing. Studies on the conformational role of antigen, anti-IgE mAbs and FcεRI binding to IgE have been carried out by the groups of Stadler and Kricek [61, 62, 63, 64]. A common theme in these reports is a putative conformational variability by IgE in its Fc region, a feature which has been predicted also by the analysis of the existing 3D structures [1].…”