Interaction of hnRNP A2/B1 Isoforms with Telomeric ssDNA and the in Vitro Function

Kamma, Hiroshi; Fujimoto, Manato; Fujiwara, Masachika; Matsui, Miwa; Horiguchi, Hisashi; Hamasaki, Makoto; Satoh, Hiroaki

doi:10.1006/bbrc.2000.4173

Cited by 50 publications

(33 citation statements)

References 22 publications

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“…A1 binds to the single-stranded telomeric DNA repeat sequence TAGGGT (a sequence almost identical to the high-affinity SELEXbinding site identified for A1 using RNA), where it is capable of stimulating telomerase activity (Burd and Dreyfuss 1994;Zhang et al 2006). A similar function has been shown for hnRNP A2 (Kamma et al 2001). hnRNP A1 shuttles between the nucleus and cytoplasm, and also has a well-established role in regulating translation of mRNAs through its binding to internal ribosome entry sites (IRES), present in the 59 UTR of many mRNAs.…”

Section: Hnrnp and Sr Proteins In Proliferation And Cancermentioning

confidence: 72%

Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged

David

Manley²

2010

Genes Dev.

720

667

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Alternative splicing of mRNA precursors is a nearly ubiquitous and extremely flexible point of gene control in humans. It provides cells with the opportunity to create protein isoforms of differing, even opposing, functions from a single gene. Cancer cells often take advantage of this flexibility to produce proteins that promote growth and survival. Many of the isoforms produced in this manner are developmentally regulated and are preferentially re-expressed in tumors. Emerging insights into this process indicate that pathways that are frequently deregulated in cancer often play important roles in promoting aberrant splicing, which in turn contributes to all aspects of tumor biology.

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Section: Hnrnp and Sr Proteins In Proliferation And Cancermentioning

confidence: 72%

Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged

David

Manley²

2010

Genes Dev.

720

667

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“…However, multiple peaks of m6A enrichment were identified within the 5 ′ ends of PCDH10 and HOXD10 transcripts, two targets of HOTAIR, which suggests that the modification could play a role in hnRNPA2/B1 selection of specific RNA transcripts. Since hnRNP A2 and B1 are identical in their RNA recognition motifs, they have primarily been described as regulating the same targets, although the B1 isoform shows stronger affinity than A2 for telomeric DNA (Kamma et al 2001). The unique 12-amino acid region of the B1 isoform may also confer structural changes that enable stronger interaction with HOTAIR.…”

Section: Transcript-specific Recruitment Of the B1 Isoformmentioning

confidence: 99%

An RNA matchmaker protein regulates the activity of the long noncoding RNA HOTAIR

Meredith

Balas

Sindy

et al. 2016

RNA

110

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The human long noncoding RNA (lncRNA) HOTAIR acts in trans to recruit the Polycomb repressive complex 2 (PRC2) to the HOXD gene cluster and to promote gene silencing during development. In breast cancers, overexpression of HOTAIR increases metastatic potential via the repression of many additional genes. It has remained unclear what factors determine HOTAIR-dependent PRC2 activity at specific genomic loci, particularly when high levels of HOTAIR result in aberrant gene silencing. To identify additional proteins that contribute to the specific action of HOTAIR, we performed a quantitative proteomic analysis of the HOTAIR interactome. We found that the most specific interaction was between HOTAIR and the heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1, a member of a family of proteins involved in nascent mRNA processing and RNA matchmaking. Our data suggest that A2/B1 are key contributors to HOTAIR-mediated chromatin regulation in breast cancer cells: A2/B1 knockdown reduces HOTAIR-dependent breast cancer cell invasion and decreases PRC2 activity at the majority of HOTAIR-dependent loci. We found that the B1 isoform, which differs from A2 by 12 additional amino acids, binds with highest specificity to HOTAIR. B1 also binds chromatin and associates preferentially with RNA transcripts of HOTAIR gene targets. We furthermore demonstrate a direct RNA-RNA interaction between HOTAIR and a target transcript that is enhanced by B1 binding. Together, these results suggest a model in which B1 matches HOTAIR with transcripts of target genes on chromatin, leading to repression by PRC2.

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“…17 A role of BC200 in neurological disorders is further supported by its direct interaction with the fragile X mental retardation both telomerase RNA 40 and telomeric DNA repeats. 41 Likewise, the telomere repeat factor TRF2 forms a stable complex with telomere-repeat-encoding RNA (TERRA) and telomere DNA repeats. 42 The strategies used to probe these interactions should be applied to the lncRNA field to determine whether these formations occur beyond the telomere.…”

Section: Mechanisms For Targeting Of Long Noncoding Rnasmentioning

confidence: 99%

Long noncoding RNA in genome regulation

2010

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Interaction of hnRNP A2/B1 Isoforms with Telomeric ssDNA and the in Vitro Function

Cited by 50 publications

References 22 publications

Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged

Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged

An RNA matchmaker protein regulates the activity of the long noncoding RNA HOTAIR

Long noncoding RNA in genome regulation

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