Interaction of high density lipoprotein particles with membranes containing cholesterol

Sánchez, Susana A.; Tricerri, M. Alejandra; Gratton, Enrico

doi:10.1194/jlr.m600457-jlr200

Cited by 38 publications

(71 citation statements)

References 64 publications

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“…In lipid membranes the Laurdan probe is sensitive to the amount of water molecules present within the bilayer. If the lipids are ordered, water molecules will have less access to the probes embedded in the membrane, thus resulting in a high value of GP [29]. The results we obtained indicate that Q extract causes increased packing order of the lipid heads at the membrane interphases.…”

Section: Packing Order and Fluidity Of Membranesupporting

confidence: 62%

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

Strugała¹,

Cyboran-Mikołajczyk²,

Wyspiańska³

et al. 2017

Rec. Nat. Prod.

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Abstract:The study is concerned with biological activity of quince (Q) fruit extract (Cydonia oblonga Mill.) towards the phosphatidylcholine liposome and the natural lipid-protein erythrocyte membrane, as well as will explaining the40 way of interaction of the extract with the membrane. The results showed that Q extract protects lipids against oxidation induced by AAPH compound to a similar extent in two models of membrane. Studies with probes (Laurdan and DPH) located at different depths within the membrane lipid bilayer showed that extract caused an increase of the packing order of the polar heads of lipids and a slight decrease in mobility of the acyl chains. Such results suggest that extract molecules associate with the lipid and lipid-protein membrane and can stop the propagation of free radicals within the bilayer by modifying the membrane fluidity. Furthermore, Q extract resulted in the inhibition of the activity of enzymes (cyclooxygenase-1 and cyclooxygenase-2) probably involved in inflammatory reactions in the body. The experimental results proved that extract components can bind to the main plasma protein -human serum albumin, and the quenching mechanism was suggested as static. The obtained quince-albumin binding constants show that the extract probably can be transported from the circulatory system to reach its target organ.

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Section: Packing Order and Fluidity Of Membranesupporting

confidence: 62%

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

Strugała¹,

Cyboran-Mikołajczyk²,

Wyspiańska³

et al. 2017

Rec. Nat. Prod.

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“…This shift can be quantified as values ranging between -1 and 1 using the GP function 134 . All bilayers of the polymerosomes appeared bluish indicating that they are significantly more hydrophobic than DOPC liposomes which appeared green 95 , see Figure 11.…”

Section: Polaritymentioning

confidence: 99%

Selecting analytical tools for characterization of polymersomes in aqueous solution

Habel

Ogbonna²,

Larsen

et al. 2015

RSC Adv.

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Selecting the appropriate analytical methods for characterizing the assembly and morphology of polymer-based vesicles, or polymersomes are required to reach their full potential in biotechnology. This work presents and compares 17 different techniques for their ability to adequately report size, lamellarity, elastic properties, bilayer surface charge, thickness and polarity of polybutadienepolyethylene oxide (PB-PEO) based polymersomes. The techniques used in this study are broadly divided into scattering techniques, visualization methods, physical and electromagnetical manipulation, sorting/purification, and simulation tools. Of the analytical methods tested, Cryo-TEM and AFM turned out to be advantageous for polymersomes with smaller diameter than 200 nm, whereas confocal microscopy is ideal for diameters > 400nm. Polymersomes in the intermediate diameter range can be characterized using FF-Cryo-SEM and NTA. SAXS provides reliable data on bilayer thickness and internal structure, Cryo-TEM on multilamellarity. Taken together, these tools are valuable for characterizing polymersomes per se but the comparative overview is also intended to serve as a starting point for selecting methods for characterizing polymersomes with encapsulated compounds or polymersomes with incorporated biomolecules (e.g. membrane proteins).

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“…42 ), and their effects on membrane properties have been explored using laurdan GP analysis. It has been shown that MbCD increases the fl uidity of biological membranes as assessed by a signifi cant reduction of laurdan GP ( 43 ).…”

Section: C-laurdan Gp Measurements In Guvsmentioning

confidence: 99%

Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope

Traian

Flecha

Levi

2012

Journal of Lipid Research

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Interaction of high density lipoprotein particles with membranes containing cholesterol

Cited by 38 publications

References 64 publications

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

Interactions of Bioactive Quince (Cydonia oblonga Mill.) Extract with Biomolecules

Selecting analytical tools for characterization of polymersomes in aqueous solution

Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscope

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