Abstract:Methods are described for the rapid determination of free ATP and ATP loosely-and firmlybound to oxygenated hemoglobin using anion exchange resin and ultrafiltration. I n hemolyzates of human erythrocytes under simulated in vivo conditions about 200/, of total ATP is firmly-bound t o hemoglobin with an association constant of about 1 x lo8 (M-1).Depending on the total ATP concentration used, equilibrium between free and firmly-bound ATP is reached within 0.2 and 0.5 sec after ATP addition to "stripped" hemolyz… Show more
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