Interaction of gentamicin polycation with model and cell membranes

Kovács, Eugenia; Savopol, Tudor; Iordache, Maria-Minodora; Săplăcan, Lavinia; Sobaru, Iuliana; Istrate, Claudia Mihaela; Mingeot‐Leclercq, Marie‐Paule; Moisescu, Mihaela G.

doi:10.1016/j.bioelechem.2012.03.001

Cited by 30 publications

(15 citation statements)

References 41 publications

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“…The loading of the MTFs with gentamicin seems to cancel the positive action of hrBMP‐2 on cell proliferation. Previous studies have shown that the interaction of gentamicin with channels and receptors in the cell membrane could lead to a decreased availability of the receptors of the BMP‐2.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants

Escobar

Muzzio

Coy

et al. 2019

Adv Materials Inter

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Novel approaches are needed to avoid bacterial infections following implant surgery. Here the use of mesoporous titania films (MTFs) for gentamicin loading and delivery and the surface functionalization of MFTs with human recombinant bone morphogenetic protein 2 (hrBMP‐2) are discussed. Gentamicin is incorporated into the MTF pores by immersion of the porous materials in gentamicin solution while hrBMP‐2 is adsorbed on top of the MTF. Contact angle and X‐ray photoelectron spectroscopy measurements are performed to prove gentamicin loading and hrBMP‐2 functionalization. An initial burst release of gentamicin takes place in physiological media followed by a prolonged release that lasts weeks. Such a release profile is highly appealing for bone implants where a high concentration of antibiotics is necessary during implant surgery while a lower antibiotic concentration is needed until tissue is regenerated. The MTFs loaded with gentamicin and functionalized with hrBMP‐2 are effective against Staphylococcus aureus colonization, and the presence of hrBMP‐2 enhances MC3T3‐E1 preosteoblastic cell attachment, proliferation, and differentiation.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants

Escobar

Muzzio

Coy

et al. 2019

Adv Materials Inter

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“…Gentamicin (in general aminoglycosides) is transported to kidney cells by endocytosis and some transporters in kidney proximal tubules membrane . Furthermore, it has been shown that gentamicin is accumulated in tubular cells' plasma membrane lipids, an event which finally leads to cell death (Figure ) . The distribution of nanosized gentamicin to tubular cells might be different from the conventional forms.…”

Section: Discussionmentioning

confidence: 99%

A Comparison between the Nephrotoxic Profile of Gentamicin and Gentamicin Nanoparticles in Mice

Jamshidzadeh

Heidari

Mohammadi-Samani

et al. 2014

J Biochem & Molecular Tox

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Aminoglycoside antibiotics are widely used against Gram-negative infections. On the other hand, nephrotoxicity is a deleterious side effect associated with aminoglycoside therapy. Gentamicin is the most nephrotoxic aminoglycoside. Because of serious health complications ensue the nephrotoxicity induced by aminoglycosides, finding new therapeutic strategies against this problem has a great clinical value. This study has attempted to compare the nephrotoxic properties of gentamicin and a new nanosized formulation of this drug in a mice model. Animals were treated with gentamicin (100 mg/kg, i.p. for eight consecutive days) and nanogentamicin (100 mg/kg, i.p. for eight consecutive days). Blood urea nitrogen (BUN), plasma creatinine levels, and histopathological changes of kidney proximal tubule were monitored. It was found that gentamicin caused severe degeneration of kidney proximal tubule cells and an increase in serum creatinine and BUN. No severe injury was observed after nanogentamicin administration. This study proved that nanosized gentamicin is less nephrotoxic.

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“…In the previous system, the coaxial cable setup used as antenna was in close proximity to the biological sample, creating a hot spot with very high localized SAR values (values higher than 1800 W/kg for 1 W input power) [27]. In that case, the mean SAR value in the solution was around 500 W/kg for 1 W input power.…”

Section: Resultsmentioning

confidence: 99%

“…Laurdan labeled SUVs were prepared according to [27]. Briefly, the phospholipid 1, 2-dimyristoyl-snglycero-3-phosphocholine (DMPC) was dissolved in chloroform, dried under nitrogen atmosphere to prevent oxidation, and resuspended in water at a final concentration of 2 mM.…”

Section: Fluorescence Measurements Protocolmentioning

confidence: 99%

Setup for Simultaneous Microwave Heating and Real-Time Spectrofluorometric Measurements in Biological Systems

Kohler¹,

Ticaud²,

Iordache³

et al. 2014

PIER

Self Cite

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Abstract-In this paper, a delivery system allowing simultaneous microwave heating and real-time spectrofluorometric measurements in biological systems is proposed and characterized. This system is used to investigate the phase behavior of lipid bilayers from about 15 • C to 45 • C. The delivery system is based on an open transverse electromagnetic (TEM) cell combined with a spectrofluorometer via an optical cable system. A numerical and experimental dosimetry of the delivery system is conducted. The Specific Absorption Rate (SAR) efficiency of the system is 26.1 ± 2.1 W/kg/W. Spectrofluorometric measurements on Laurdan labeled small unilamellar vesicles (SUVs) are carried out. Generalized polarization (GP) of the SUV's membrane is obtained from the fluorescence intensities measured at two emission wavelengths.

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Interaction of gentamicin polycation with model and cell membranes

Cited by 30 publications

References 41 publications

Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants

Antibacterial Mesoporous Titania Films with Embedded Gentamicin and Surface Modified with Bone Morphogenetic Protein 2 to Promote Osseointegration in Bone Implants

A Comparison between the Nephrotoxic Profile of Gentamicin and Gentamicin Nanoparticles in Mice

Setup for Simultaneous Microwave Heating and Real-Time Spectrofluorometric Measurements in Biological Systems

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