Interaction of functionalized nanoparticles with serum proteins and its impact on colloidal stability and cargo leaching

Abstiens, Kathrin; Figueroa, Sara Maslanka; Gregoritza, Manuel; Goepferich, Achim

doi:10.1039/c8sm02189a

Cited by 63 publications

(65 citation statements)

References 41 publications

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“…[61,62] In our study, the formation of a protein corona around AgNPs after the addition of a food matrix was also evidenced after protein quantification with the micro-BCA assay. This method was previously used in other studies [64][65][66] and allows to confirm that the alterations of the hydrodynamic diameter, the zeta-potential and the SPR peak of AgNPs in the presence of food components can be attributable to the binding of BSA molecules to the nanoparticles.…”

Section: Discussionmentioning

confidence: 94%

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Laloux

Kastrati

Cambier

et al. 2020

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Silver nanoparticles (AgNPs) are used in the agri‐food sector, which can lead to their ingestion. Their interaction with food and their passage through the gastrointestinal tract can alter their properties and influence their fate upon ingestion. Therefore, this study aims at developing an in vitro method to follow the fate of AgNPs in the gastrointestinal tract. After incorporation of AgNPs into a standardized food matrix, a precolonic digestion is simulated and AgNPs are characterized by different techniques. The presence of food influences the AgNPs properties by forming a corona around nanoparticles. Even if the salivary step does not impact significantly the AgNPs, the pH decrease and the digestive enzymes induce the agglomeration of AgNPs during the gastric phase, while the addition of intestinal fluids disintegrates these clusters. AgNPs can thus reach the intestinal cells under nanometric form, although the presence of food and gastrointestinal fluids modifies their properties compared to pristine AgNPs. They can form a corona around the nanoparticles and act as colloidal stabilizer, which can impact the interaction of AgNPs with intestinal epithelium. This study demonstrates the importance of taking the fate of AgNPs in the gastrointestinal tract into account to perform an accurate risk assessment of nanomaterials.

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Section: Discussionmentioning

confidence: 94%

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Laloux

Kastrati

Cambier

et al. 2020

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“…Polymer Synthesis and NP Preparation. To synthesize block copolymers with different functional groups at the end of the PEG chain, we used commercially obtained carboxylic acid-or methoxy-terminated PEG as macroinitiators for ring-opening polymerization of cyclic lactide using 1,8-diazabicyclo [5.4.0] undec-7-ene (DBU) as a catalyst after Qian et al (23) with slight modifications, as previously described by our group (46). Resulting block copolymers were a 10-kDa PLA with a carboxylic acid-terminated 2-kDa or 5-kDa PEG chain or a methoxy-terminated 5-kDa PEG chain.…”

Section: Methodsmentioning

confidence: 99%

Influenza A virus mimetic nanoparticles trigger selective cell uptake

Figueroa

Veser

Abstiens

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Poor target cell specificity is currently a major shortcoming of nanoparticles (NPs) used for biomedical applications. It causes significant material loss to off-target sites and poor availability at the intended delivery site. To overcome this limitation, we designed particles that identify cells in a virus-like manner. As a blueprint, we chose a mechanism typical of influenza A virus particles in which ectoenzymatic hemagglutinin activation by target cells is a mandatory prerequisite for binding to a secondary target structure that finally confirms cell identity and allows for uptake of the virus. We developed NPs that probe mesangial cells for the presence of angiotensin-converting enzyme on their surface using angiotensin I (Ang-I) as a proligand. This initial interaction enzymatically transforms Ang-I to a secondary ligand angiotensin II (Ang-II) that has the potential to bind in a second stage to Ang-II type-1 receptor (AT1R). The presence of the receptor confirms the target cell identity and triggers NP uptake via endocytosis. Our virus-mimetic NPs showed outstanding target-cell affinity with picomolar avidities and were able to selectively identify these cells in the presence of 90% off-target cells that carried only the AT1R. Our results demonstrate that the design of virus-mimetic cell interactive NPs is a valuable strategy to enhance NP specificity for therapeutic and diagnostic applications. Our set of primary and secondary targets is particularly suited for the identification of mesangial cells that play a pivotal role in diabetic nephropathy, one of the leading causes of renal failure, for which currently no treatment exists.

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“…However, the overall protein adsorption, colloidal stability, and lower uptake by macrophages still required higher percentages of polymer to be achieved, demonstrating how a good NPs surface coverage is paramount to achieve optimal NP stealthiness. Similarly, Abstienze et al analyzed the assembly of PC on different PEG-polylactic acid (PEG-PLA) NPs (Abstiens et al, 2019). These NPs presented on their surfaces different combinations of PEG terminal groups: positively charged amines, negatively charged carboxyl groups, a combination of the two (zwitterionic), or non-charged methoxy groups.…”

Section: Synthetic Approachesmentioning

confidence: 99%

Recent Advances in Understanding the Protein Corona of Nanoparticles and in the Formulation of “Stealthy” Nanomaterials

Rampado

Crotti

Caliceti

et al. 2020

Front. Bioeng. Biotechnol.

234

166

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In the last decades, the staggering progress in nanotechnology brought around a wide and heterogeneous range of nanoparticle-based platforms for the diagnosis and treatment of many diseases. Most of these systems are designed to be administered intravenously. This administration route allows the nanoparticles (NPs) to widely distribute in the body and reach deep organs without invasive techniques. When these nanovectors encounter the biological environment of systemic circulation, a dynamic interplay occurs between the circulating proteins and the NPs, themselves. The set of proteins that bind to the NP surface is referred to as the protein corona (PC). PC has a critical role in making the particles easily recognized by the innate immune system, causing their quick clearance by phagocytic cells located in organs such as the lungs, liver, and spleen. For the same reason, PC defines the immunogenicity of NPs by priming the immune response to them and, ultimately, their immunological toxicity. Furthermore, the protein corona can cause the physical destabilization and agglomeration of particles. These problems induced to consider the PC only as a biological barrier to overcome in order to achieve efficient NP-based targeting. This review will discuss the latest advances in the characterization of PC, development of stealthy NP formulations, as well as the manipulation and employment of PC as an alternative resource for prolonging NP half-life, as well as its use in diagnostic applications.

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Interaction of functionalized nanoparticles with serum proteins and its impact on colloidal stability and cargo leaching

Abstract: Formation of a protein corona can severely compromise the performance of functionalized polymeric nanoparticles by changing their identity and integrity.

Cited by 63 publications

References 41 publications

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Influenza A virus mimetic nanoparticles trigger selective cell uptake

Recent Advances in Understanding the Protein Corona of Nanoparticles and in the Formulation of “Stealthy” Nanomaterials

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