Interaction of Frizzled 7 and Dishevelled inXenopus

Medina, Araceli; Steinbeißer, Herbert

doi:10.1002/1097-0177(2000)9999:9999<::aid-dvdy1017>3.0.co;2-9

Cited by 32 publications

(8 citation statements)

References 37 publications

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“…The intracellular domain of BAMBI is important for the interaction with all three proteins while the extracellular domain can associate only with Fzd, which is consistent with the topological structure of BAMBI as a single-spanned transmembrane protein. It has been demonstrated that Wnt treatment results in the plasma membrane recruitment of Dvl (34), but the binding of Dvl to Fzd could be relatively weak (40). Our data uncovered that BAMBI can increase the interaction between Fzd and Dvl as this interaction became detectable only in the presence of BAMBI in our experimental condition (Fig.…”

Section: Discussionmentioning

confidence: 81%

“…Wnt signal is propagated following the binding of Wnt ligands to a hetero-oligomeric receptor complex consisting of Fzd and LRP5/6 (29 -31), which results in the recruitment of Axin to LRP (32,33) and Dvl to Fzd (29,34). However, the interaction between Dvl and Frizzled is apparently weak, and it is unclear whether other proteins are involved in this process.…”

Section: Bambi Enhances the Interaction Of Fzd5 And Dvl2-thementioning

confidence: 99%

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The Pseudoreceptor BMP and Activin Membrane-bound Inhibitor Positively Modulates Wnt/β-Catenin Signaling

Lin

Gao

Ning

et al. 2008

Journal of Biological Chemistry

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The canonical Wnt/␤-catenin pathway plays a pivotal role in regulating embryogenesis and tumorigenesis by promoting cell proliferation. BAMBI (BMP and activin membrane-bound inhibitor) has previously been shown to negatively regulate the signaling activity of transforming growth factor-␤, activin, and BMP and was identified as a target of ␤-catenin in colorectal and hepatocellular tumor cells. In this study, we provide evidence that BAMBI can promote the transcriptional activity of Wnt/␤-catenin signaling. Overexpression of BAMBI enhances the expression of Wnt-responsive reporters, whereas knockdown of endogenous BAMBI attenuates them. Accordingly, BAMBI also promotes the nuclear translocation of ␤-catenin. BAMBI interacts with Wnt receptor Frizzled5, coreceptor LRP6, and Dishevelled2 and increases the interaction between Frizzled5 and Dishevelled2. Finally we show that BAMBI promotes the expression of c-myc and cyclin D1 and increases Wnt-promoted cell cycle progression. Altogether, our data indicate that BAMBI can function as a positive regulator of the Wnt/␤-catenin pathway to promote cell proliferation.Wnt signaling is initiated by binding to its seven-transmembrane receptor, Frizzled (Fzd), 2 and single-span transmembrane coreceptor low density lipoprotein receptor-related protein 5 and 6 (LRP5/6) (1-4). Dishevelled (Dvl) and ␤-catenin play an essential role in the canonical Wnt signaling. In the absence of Wnt proteins, ␤-catenin is phosphorylated (5-11) and ubiquitinated and then targeted for proteasome-mediated degradation by a cytoplasmic destruction complex consisting of adenomatous polyposis coli, Axin, glycogen synthase kinase 3␤ (GSK3␤), and casein kinase 1. The binding of Wnt ligands to the receptors results in the recruitment of Dvl to the plasma membrane and therefore the disassembly of the destructive complex, leading to the accumulation and nuclear translocation of ␤-catenin, which collaborates with the transcription factors of the lymphoid enhancer-binding factor (LEF)/T-cell factor (TCF) family and activates the transcription of Wnt target genes.The Wnt signaling pathway plays key roles in embryogenesis and adult homeostasis and is aberrantly activated in various human cancers (3, 4). For instance, in most of colorectal cancers, ␤-catenin is stabilized and accumulated in the nucleus and constitutively activates its target genes (12, 13). A wide spectrum of ␤-catenin targets, such as the proteins involved in cell cycle, cell-matrix interactions, migration, and invasion, has been identified (14 -16). One of such targets is BAMBI (BMP and activin membrane-bound inhibitor), which has been found to be directly up-regulated by the functional ␤-catenin-TCF4 complex in colorectal tumor cells (17).The BAMBI gene encodes a 260-amino acid transmembrane protein that is evolutionally conserved in vertebrates, from fish to human (18 -22). The BAMBI protein is closely related to type I receptors of the transforming growth factor-␤ (TGF-␤) family in the extracellular domain but has a short intracellular doma...

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Section: Discussionmentioning

confidence: 81%

Section: Bambi Enhances the Interaction Of Fzd5 And Dvl2-thementioning

confidence: 99%

The Pseudoreceptor BMP and Activin Membrane-bound Inhibitor Positively Modulates Wnt/β-Catenin Signaling

Lin

Gao

Ning

et al. 2008

Journal of Biological Chemistry

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“…pCS2+ xFz7 encodes the full-length xFz7 (Medina and Steinbeisser, 2000). pCS2+ ΔCxFz7 lacks the 26 C-terminal amino acids, pCS2+ NxFz7-fun comprises the N-terminal domain of xFz7 fused with a fun domain (Medina et al, 2000), and pCS2+ ΔNxFz7 encodes the seven transmembrane domains and the C tail of xFz7 (Winklbauer et al, 2001).…”

Section: Methodsmentioning

confidence: 99%

Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin

Kraft

Berger

Wallkamm

et al. 2012

Journal of Cell Biology

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“…Here, we show that Dpr1 interacts with Dvl and enhances Dvl degradation in a lysosome inhibitorsensitive and proteasome inhibitor-insensitive manner in an analogous way to the activity of Dpr2 that associates with transforming growth factor-␤ type I receptors and targets them for lysosomal degradation (10). Dvl shuttles between the cytoplasm and the nucleus (22) and can also be recruited to the plasma membrane (23,24). How Dpr1 promotes Dvl degradation awaits further investigation.…”

Section: Interaction Between Mammalian Dpr1 and Dvl At The Endogenousmentioning

confidence: 98%

Dapper 1 Antagonizes Wnt Signaling by Promoting Dishevelled Degradation

Zhang

Gao

Wen

et al. 2006

Journal of Biological Chemistry

138

181

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Interaction of Frizzled 7 and Dishevelled inXenopus

Cited by 32 publications

References 37 publications

The Pseudoreceptor BMP and Activin Membrane-bound Inhibitor Positively Modulates Wnt/β-Catenin Signaling

The Pseudoreceptor BMP and Activin Membrane-bound Inhibitor Positively Modulates Wnt/β-Catenin Signaling

Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin

Dapper 1 Antagonizes Wnt Signaling by Promoting Dishevelled Degradation

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